Human Cytokine Array C6

$436.00
AAH-CYT-6-2

RayBio® C-Series Human Cytokine Antibody Array 6 Kit. Detects 60 Human Cytokines. Suitable for all liquid sample types.

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Antigen Information

Gene Symbol:
ANG | BDNF | BMP4 | BMP6 | CCL1 | CCL11 | CCL13 | CCL15 | CCL17 | CCL18   View more

Assay Format

Number of Targets Detected:
60
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Angiogenin
BDNF
BLC
(CXCL13)
BMP-4
BMP-6
Ck beta 8-1
(CCL23)
CNTF
EGF
Eotaxin-1
(CCL11)
Eotaxin-2
(MPIF-2/CCL24)
Eotaxin-3
(CCL26)
FGF-6
FGF-7
(KGF)
Flt-3 Ligand
Fractalkine
(CX3CL1)
GCP-2
(CXCL6)
GDNF
GM-CSF
I-309
(TCA-3/CCL1)
IFN-gamma
IGFBP-1
IGFBP-2
IGFBP-4
IGF-1
IL-10
IL-13
IL-15
IL-16
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-1 ra
(IL-1 F3)
IL-2
IL-3
IL-4
IL-5
IL-6
IL-7
Leptin
Light
(TNFSF14)
MCP-1
(CCL2)
MCP-2
(CCL8)
MCP-3
(MARC/CCL7)
MCP-4
(CCL13)
M-CSF
MDC
(CCL22)
MIG
(CXCL9)
MIP-1 delta
(CCL15)
MIP-3 alpha
(CCL20)
NAP-2
(PPBP/CXCL7)
NT-3
PARC
(CCL18)
PDGF-BB
RANTES
(CCL5)
SCF
SDF-1 alpha
(CXCL12 alpha)
TARC
(CCL17)
TGF beta 1
TGF beta 3
  • Gene Symbol: TGFB3
  • Gene ID: 7043
  • Accession #: P10600
TNF alpha
TNF beta
(TNFSF1B)

Species Detected

Human

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Cytokine Antibody Array C6 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP??s ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Shang T., Yao B., Gao D., et al. A novel model of humanised keloid scarring in mice. Int Wound J. 2018 Feb;15(1):90-94. doi: 10.1111/iwj.12838.
    Species: Human
    Sample type: Conditioned Media
  2. Li MF., Zhang R., Li TT., et al. High Glucose Increases the Expression of Inflammatory Cytokine Genes in Macrophages Through H3K9 Methyltransferase Mechanism. J Interferon Cytokine Res. 2016 Jan;36(1):48-61. doi: 10.1089/jir.2014.0172
    Species: Human
    Sample type: Conditioned Media
  3. Jin H., Lee H., Heo J., et al. Senescence-Associated MCP-1 Secretion Is Dependent on a Decline in BMI1 in Human Mesenchymal Stromal Cells. Antioxid Redox Signal. 2016 Mar 20;24(9):471-85. doi: 10.1089/ars.2015.6359.
    Species: Human
    Sample type: Conditioned Media
  4. Longhin E., Capasso L., Battaglia C., et al. Integrative transcriptomic and protein analysis of human bronchial BEAS-2B exposed to seasonal urban particulate matter. Environmental Pollution Volume 209, February 2016, Pages 87-98
    Species: Human
    Sample type: Cell Lysate
  5. Lee J., Schierer S., Blume K., et al. HIV-Nef and ADAM17-Containing Plasma Extracellular Vesicles Induce and Correlate with Immune Pathogenesis in Chronic HIV Infection. EBioMedicine. 2016 Apr; 6: 103-113.
    Species: Human
    Sample type: Extracellular Vesicles
  6. Balakumaran A., Mishra PJ., Pawelczyk E., et al. Bone marrow skeletal stem/progenitor cell defects in patients with dyskeratosis congenita and telomere biology disorders. Blood. 2015 Jan 29;125(5):793-802. doi: 10.1182/blood-2014-06-566810.
    Species: Human
    Sample type: Conditioned Media
  7. Blauth K., Zhang X., Chopra M., et al. The role of fractalkine (CX3CL1) in regulation of CD4+ cell migration to the central nervous system in patients with relapsing-remitting multiple sclerosis. Clinical Immunology, Available online 14 January 2015, doi:10.1016/j.clim.2015.01.001
    Species: Human
    Sample type: CSF
  8. Lejmi E., Perriraz N., Clement S., et al. Inflammatory Chemokines MIP-1d and MIP-3a Are Involved in the Migration of Multipotent Mesenchymal Stromal Cells Induced by Hepatoma Cells.
    Species: Human
    Sample type: Conditioned Media
  9. Loria F., Diaz-Nido J. Frataxin knockdown in human astrocytes triggers cell death and the release of factors that cause neuronal toxicity. Neurobiology of Disease, 76 (2015) 1-12. doi:10.1016/j.nbd.2014.12.017
    Species: Human
    Sample type: Conditioned Media
  10. Moore MC., Pandolfi V., McFetridge P. Novel human-derived extracellular matrix induces in vitro and in vivo vascularization and inhibits fibrosis. Biomaterials. 2015 May;49:37-46. doi: 10.1016/j.biomaterials.2015.01.022.
    Species: Human
    Sample type: Conditioned Media

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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