Human Cytokine Array C6

RayBio® C-Series Human Cytokine Antibody Array 6 Kit. Detects 60 Human Cytokines. Suitable for all liquid sample types.

$440.36  
AAH-CYT-6-2
+

Antigen Information

Gene Symbols:
ANG | BDNF | BMP4 | BMP6 | CCL1 | CCL11 | CCL13 | CCL15 | CCL17 | CCL18   View more

Assay Format

Number of Targets Detected:
60
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Angiogenin
BDNF
BLC
(CXCL13)
BMP-4
BMP-6
Ck beta 8-1
(CCL23)
CNTF
EGF
Eotaxin-1
(CCL11)
Eotaxin-2
(MPIF-2/CCL24)
Eotaxin-3
(CCL26)
FGF-6
FGF-7
(KGF)
Flt-3 Ligand
Fractalkine
(CX3CL1)
GCP-2
(CXCL6)
GDNF
GM-CSF
I-309
(TCA-3/CCL1)
IFN-gamma
IGFBP-1
IGFBP-2
IGFBP-4
IGF-1
IL-10
IL-13
IL-15
IL-16
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-1 ra
(IL-1 F3)
IL-2
IL-3
IL-4
IL-5
IL-6
IL-7
Leptin
Light
(TNFSF14)
MCP-1
(CCL2)
MCP-2
(CCL8)
MCP-3
(MARC/CCL7)
MCP-4
(CCL13)
M-CSF
MDC
(CCL22)
MIG
(CXCL9)
MIP-1 delta
(CCL15)
MIP-3 alpha
(CCL20)
NAP-2
(PPBP/CXCL7)
NT-3
PARC
(CCL18)
PDGF-BB
RANTES
(CCL5)
SCF
SDF-1 alpha
(CXCL12 alpha)
TARC
(CCL17)
TGF beta 1
TGF beta 3
  • Gene Symbol: TGFB3
  • Gene ID: 7043
  • Accession #: P10600
TNF alpha
TNF beta
(TNFSF1B)

Species Detected

Human

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Cytokine Antibody Array C6 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP??s ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Deng W., et al. Down-modulation of TNFSF15 in ovarian cancer by VEGFand MCP-1 is a pre-requisite for tumor neovascularization. Angiogenesis (2012) 15:71-85 DOI 10.1007/s10456-011-9244-y
    Species: Human
    Sample type: Conditioned Media
  2. Ho L, Zhao W, Dams-O’Connor K, et al. Elevated Plasma MCP-1 Concentration Following Traumatic Brain Injury as a Potential “Predisposition” Factor Associated with an Increased Risk for Subsequent Development of Alzheimer’s Disease. Journal of Alzheimer’s disease?: JAD. 2012;31(2):301-313. doi:10.3233/JAD-2012-120598.
    Species: Human
    Sample type: Plasma
  3. Hsiao WC., Sung SY., Lioa CH., et al. Vitamin D3-inducible mesenchymal stem cell-based delivery of conditionally replicating adenoviruses effectively targets renal cell carcinoma and inhibits tumor growth. Mol Pharm. 2012 May 7;9(5):1396-408. doi: 10.1021/mp200649g.
    Species: Human
    Sample type: Conditioned Media
  4. Hwang YS., Xianglan Z., Park KK., Chung WY. Functional invadopodia formation through stabilization of the PDPN transcript by IMP-3 and cancer-stromal crosstalk for PDPN expression. Carcinogenesis. 2012 Nov;33(11):2135-46. doi: 10.1093/carcin/bgs258
    Species: Human
    Sample type: Conditioned Media
  5. Jones ML., Ewing CM., Isaacsa WB., Getzenberg RH. Prostate cancer-derived angiogenin stimulates the invasion of prostate fibroblasts. J Cell Mol Med. 2012 Jan;16(1):193-201. doi: 10.1111/j.1582-4934.2011.01283.x.
    Species: Human
    Sample type: Conditioned Media
  6. Mason GM, Poole E, Sissons JGP, Wills MR, Sinclair JH. Human cytomegalovirus latency alters the cellular secretome, inducing cluster of differentiation (CD)4+ T-cell migration and suppression of effector function. Proceedings of the National Academy of Sciences of the United States of America 2012;109(36):14538-14543. doi:10.1073/pnas.1204836109.
    Species: Human
    Sample type: Conditioned Media
  7. Pickard A, Cichon A-C, Barry A, et al. Inactivation of Rb in stromal fibroblasts promotes epithelial cell invasion. The EMBO Journal 2012;31(14):3092-3103. doi:10.1038/emboj.2012.153.
    Species: Human
    Sample type: Conditioned Media
  8. Shi Z., Yang YM., Chen LP., et al. Enhanced chemosensitization in multidrug-resistant human breast cancer cells by inhibition of IL-6 and IL-8 production. Breast Cancer Res Treat. 2012 Oct;135(3):737-47. doi: 10.1007/s10549-012-2196-0
    Species: Human
    Sample type: Conditioned Media
  9. Shih YT., Wang MC., Peng HH., et al. Modulation of chemotactic and pro-inflammatory activities of endothelial progenitor cells by hepatocellular carcinoma. Cell Signal. 2012 Mar;24(3):779-93. doi: 10.1016/j.cellsig.2011.11.013.
    Species: Human
    Sample type: Conditioned Media
  10. Sofrenovic T., McEwan K., Crowe S., et al. Circulating angiogenic cells can be derived from cryopreserved peripheral blood mononuclear cells. PLoS One. 2012;7(10):e48067. doi: 10.1371/journal.pone.0048067
    Species: Human
    Sample type: Conditioned Media

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This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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