Human Cytokine Array C7

RayBio® C-Series Human Cytokine Antibody Array 7 Kit. Detects 60 Human Cytokines. Suitable for all liquid sample types.

$440.36  
AAH-CYT-7-2
+

Antigen Information

Gene Symbols:
ADIPOQ | AGRP | ANGPT2 | AREG | AXL | BTC | CCL16 | CCL19 | CCL25 | CCL27   View more

Assay Format

Number of Targets Detected:
60
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Adiponectin
(ACRP30)
AgRP
Angiopoietin-2
Amphiregulin
Axl
bFGF
beta-NGF
Betacellulin
(BTC)
CCL28
(MEC)
CTACK
(CCL27)
Dtk
EGFR
ENA-78
(CXCL5)
Fas
(TNFRSF6/Apo-1)
FGF-4
FGF-9
GCSF
GITR Ligand
(TNFSF18)
GITR
(TNFRSF18)
GRO alpha/beta/gamma
GRO alpha
(CXCL1)
HCC-4
(CCL16)
HGF
ICAM-1
(CD54)
ICAM-3
(CD50)
IGFBP-3
IGFBP-6
IGF-1 R
IL-1 R4
(ST2)
IL-1 R1
IL-11
IL-12 p40
IL-12 p70
IL-17A
  • Gene Symbol: IL17A
  • Gene ID: 3605
  • Accession #: Q16552
IL-2 R alpha
IL-6 R
IL-8
(CXCL8)
I-TAC
(CXCL11)
Lymphotactin
(XCL1)
MIF
MIP-1 alpha
(CCL3)
MIP-1 beta
(CCL4)
MIP-3 beta
(CCL19)
MSP alpha/beta
NT-4
Osteoprotegerin
(TNFRSF11B)
Oncostatin M
PLGF
gp130
TNF RII
(TNFRSF1B)
TNF RI
(TNFRSF1A)
TECK
(CCL25)
TIMP-1
TIMP-2
Thrombopoietin
(TPO)
TRAIL R3
(TNFRSF10C)
TRAIL R4
(TNFRSF10D)
uPAR
VEGF-A
VEGF-D

Species Detected

Human

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Cytokine Antibody Array C7 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Chung E., et al. CXCL5 abundant in the wound fluid at the late phase of wound healing, possibly promoting migration of mesenchymal stem cells and vascular tube formation. Tissue Engineering and Regenerative Medicine August 2014, Volume 11, Issue 4, pp 317-322
    Species: Human
    Sample type: Wound Fluid
  2. Jung JH., Lee SJ., Kim JH., et al. Chemokine (C-X-C Motif) Receptor 2 and Its Related Ligands Play a Novel Role in Supporting the Pluripotency and Proliferation of Human Pluripotent Stem Cells. Stem Cells Dev. 2014 Nov 12. [Epub ahead of print]: DOI: 10.1089/scd.2014.0381
    Species: Human
    Sample type: Conditioned Media
  3. Mannoji C., Koda M., Kamya K., et al. Transplantation of human bone marrow stromal cell-derived neuroregenrative cells promotes functional recovery after spinal cord injury in mice. Acta Neurobiol Exp. 2014;74(4):479-88
    Species: Human
    Sample type: Conditioned Media
  4. Pu D., Wang W. Toll?like receptor 4 agonist, lipopolysaccharide, increases the expression levels of cytokines and chemokines in human peripheral blood mononuclear cells. EXPERIMENTAL AND THERAPEUTIC MEDICINE 8: 1914-1918, 2014. DOI: 10.3892/etm.2014.2025
    Species: Human
    Sample type: Conditioned Media
  5. Shih Y., Wang M., Zhou J., Peng H., Lee D., Chiu J. Endothelial progenitors promote hepatocarcinoma intrahepatic metastasis through monocyte chemotactic protein-1 induction of microRNA-21. Gut 2014 Epub ahead of print June 17 2014, doi:10.1136/gutjnl-2013-306302
    Species: Human
    Sample type: Cell Lysate
  6. Ho Ia., Toh H., Ng W., et al. Human bone marrow-derived mesenchymal stem cells suppress human glioma growth through inhibition of angiogenesis. Stem Cells. 2013 Jan;31(1):146-55. doi: 10.1002/stem.1247
    Species: Human
    Sample type: Conditioned Media
  7. Ho I., Toh H., Ng W., et al. Human Bone Marrow?Derived Mesenchymal Stem Cells Suppress Human Glioma Growth Through Inhibition of Angiogenesis, Stem Cells. 2013 Jan;31(1):146-55. doi: 10.1002/stem.1247.
    Species: Human
    Sample type: Conditioned Media
  8. Low-Marchelli JM, Ardi VC, Vizcarra EA, et al. Twist1 Induces CCL2 and Recruits Macrophages to Promote Angiogenesis. Cancer Res. 2013;73:662-671.
    Species: Human
    Sample type: Conditioned Media
  9. Wang WE, Yang D, Li L, et al. PHD2 Silencing Enhances the Survival and Paracrine Function of Transplanted Adipose-Derived Stem Cells in Infarcted Myocardium. Circ. Res. 2013 May 21;
    Species: Human
    Sample type: Conditioned Media
  10. Zhang Y, Liu H, Wang L, Yang F, Hu Y, et al. (2013) Comparative Study of the Cytokine/Chemokine Response in Children with Differing Disease Severityin Enterovirus 71-Induced Hand, Foot, and Mouth Disease. PLoS ONE 8(6): e67430. doi:10.1371/journal.pone.0067430
    Species: Human
    Sample type: Plasma
The product is very good, easy to use. The signal is very sensitive.

University of Minnesota
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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