Human Cytokine Array C7

RayBio® C-Series Human Cytokine Antibody Array 7 Kit. Detects 60 Human Cytokines. Suitable for all liquid sample types.

$440.36  
AAH-CYT-7-2
+

Antigen Information

Gene Symbols:
ADIPOQ | AGRP | ANGPT2 | AREG | AXL | BTC | CCL16 | CCL19 | CCL25 | CCL27   View more

Assay Format

Number of Targets Detected:
60
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Adiponectin
(ACRP30)
AgRP
Angiopoietin-2
Amphiregulin
Axl
bFGF
beta-NGF
Betacellulin
(BTC)
CCL28
(MEC)
CTACK
(CCL27)
Dtk
EGFR
ENA-78
(CXCL5)
Fas
(TNFRSF6/Apo-1)
FGF-4
FGF-9
GCSF
GITR Ligand
(TNFSF18)
GITR
(TNFRSF18)
GRO alpha/beta/gamma
GRO alpha
(CXCL1)
HCC-4
(CCL16)
HGF
ICAM-1
(CD54)
ICAM-3
(CD50)
IGFBP-3
IGFBP-6
IGF-1 R
IL-1 R4
(ST2)
IL-1 R1
IL-11
IL-12 p40
IL-12 p70
IL-17A
  • Gene Symbol: IL17A
  • Gene ID: 3605
  • Accession #: Q16552
IL-2 R alpha
IL-6 R
IL-8
(CXCL8)
I-TAC
(CXCL11)
Lymphotactin
(XCL1)
MIF
MIP-1 alpha
(CCL3)
MIP-1 beta
(CCL4)
MIP-3 beta
(CCL19)
MSP alpha/beta
NT-4
Osteoprotegerin
(TNFRSF11B)
Oncostatin M
PLGF
gp130
TNF RII
(TNFRSF1B)
TNF RI
(TNFRSF1A)
TECK
(CCL25)
TIMP-1
TIMP-2
Thrombopoietin
(TPO)
TRAIL R3
(TNFRSF10C)
TRAIL R4
(TNFRSF10D)
uPAR
VEGF-A
VEGF-D

Species Detected

Human

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Cytokine Antibody Array C7 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. He, Wei, et al. "High tumor levels of IL6 and IL8 abrogate preclinical efficacy of the ??secretase inhibitor, RO4929097." Molecular oncology 5.3 (2011): 292-301.
    Species: Human
    Sample type: Conditioned Media
  2. Kim D, Cho H,Yang S, Shin J,Huh C,et al.IGFBP-2 Contributes to the Proliferation of Less Proliferative Cells in Forming Skin Equivalents.Tissue Eng Part A. 2009; 15(5):1075-1080.
    Species: Human
    Sample type: Conditioned Media
  3. Zhang X, Jin J P, Tang Y N, Speer D, Sujkowska D, et al. IFN-1a Inhibits the Secretion of Th17-Polarizing Cytokines in Human Dendritic Cells via TLR7 Up-Regulation.J Immunol. 2009;182:3928-3936
    Species: Human
    Sample type: Conditioned Media
  4. Fujita K,Ewing C M ,Sokoll L J, Elliott D J, Cunningham M, et al.Cytokine Profiling of Prostatic Fluid From Cancerous Prostate Glands Identifies Cytokines Associated With Extent of Tumor and Inflammation. Prostate. 2008;68:872-882.
    Species: Human
    Sample type: Prostatic Fluid
  5. Steed D, Trumpower C, Duffy D, Smith C, Marshall V, et al. Amnion-derived Cellular Cytokine Solution A Physiological Combination of Cytokines for Wound Healing. Eplasty. 2008;8:e18.
    Species: Human
    Sample type: Conditioned Media
  6. Traktuev D, Merfeld-Clauss S, Li J, Kolonin M, Arap W, et al.A Population of Multipotent CD34-Positive Adipose Stromal Cells Share Pericyte and Mesenchymal Surface Markers, Reside in a Periendothelial Location, and Stabilize Endothelial Networks. Circulation Research. 2008; 102: 77-85
    Species: Human
    Sample type: Conditioned Media
  7. Ukropec J, Penesova A, Kopkova M, Pura M, Vlcek M,et al.Adipokine Protein Expression Pattern in Growth Hormone Deficiency Predisposes to the Increased Fat Cell Size and the Whole Body Metabolic Derangements. J Clin Endocrinol Metab. 2008;93: 2255-2262
    Species: Human
    Sample type: Tissue Lysate
  8. Yang CC, Shih YH, Ko MH, Hsu SY,Cheng H, et al. Transplantation of Human Umbilical Mesenchymal Stem Cells from Wharton’s Jelly after Complete Transection of the Rat Spinal Cord. PLoS ONE. 2008;3(10):e3336. doi:10.1371/journal.pone.0003336
    Species: Human
    Sample type: Tissue Lysate
  9. Fu SY, Su GY, McKinley SH, Yen MT. Cytokine expression in pediatric subperiosteal orbital abscesses. Can J Ophthalmol. 2007;42:865-869.
    Species: Human
    Sample type: Abcess Fluid
  10. Hartiala P, Hytonen J, Pelkonen J, Kimppa K, et al. Transcriptional response of human dendritic cells to Borrelia garinii---defective CD38 and CCR7 expression detected. J Leuk Biol. 2007; 82:33-43.
    Species: Human
    Sample type: Conditioned Media
The product is very good, easy to use. The signal is very sensitive.

University of Minnesota
Write A Review

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

C-Series Testing Service C-Series Testing Service

Amazon Gift Card Program

Related Products Related Products