Human Cytokine Array C7

$436.00
AAH-CYT-7-2

RayBio® C-Series Human Cytokine Antibody Array 7 Kit. Detects 60 Human Cytokines. Suitable for all liquid sample types.

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Antigen Information

Gene Symbol:
ADIPOQ | AGRP | ANGPT2 | AREG | AXL | BTC | CCL16 | CCL19 | CCL25 | CCL27   View more

Assay Format

Number of Targets Detected:
60
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Adiponectin
(ACRP30)
AgRP
Angiopoietin-2
Amphiregulin
Axl
bFGF
beta-NGF
Betacellulin
(BTC)
CCL28
(MEC)
CTACK
(CCL27)
Dtk
EGFR
ENA-78
(CXCL5)
Fas
(TNFRSF6/Apo-1)
FGF-4
FGF-9
GCSF
GITR Ligand
(TNFSF18)
GITR
(TNFRSF18)
GRO alpha/beta/gamma
GRO alpha
(CXCL1)
HCC-4
(CCL16)
HGF
ICAM-1
(CD54)
ICAM-3
(CD50)
IGFBP-3
IGFBP-6
IGF-1 R
IL-1 R4
(ST2)
IL-1 R1
IL-11
IL-12 p40
IL-12 p70
IL-17A
  • Gene Symbol: IL17A
  • Gene ID: 3605
  • Accession #: Q16552
IL-2 R alpha
IL-6 R
IL-8
(CXCL8)
I-TAC
(CXCL11)
Lymphotactin
(XCL1)
MIF
MIP-1 alpha
(CCL3)
MIP-1 beta
(CCL4)
MIP-3 beta
(CCL19)
MSP alpha/beta
NT-4
Osteoprotegerin
(TNFRSF11B)
Oncostatin M
PLGF
gp130
TNF RII
(TNFRSF1B)
TNF RI
(TNFRSF1A)
TECK
(CCL25)
TIMP-1
TIMP-2
Thrombopoietin
(TPO)
TRAIL R3
(TNFRSF10C)
TRAIL R4
(TNFRSF10D)
uPAR
VEGF-A
VEGF-D

Species Detected

Human

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Cytokine Antibody Array C7 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Shang T., Yao B., Gao D., et al. A novel model of humanised keloid scarring in mice. Int Wound J. 2018 Feb;15(1):90-94. doi: 10.1111/iwj.12838.
    Species: Human
    Sample type: Conditioned Media
  2. Li MF., Zhang R., Li TT., et al. High Glucose Increases the Expression of Inflammatory Cytokine Genes in Macrophages Through H3K9 Methyltransferase Mechanism. J Interferon Cytokine Res. 2016 Jan;36(1):48-61. doi: 10.1089/jir.2014.0172
    Species: Human
    Sample type: Conditioned Media
  3. Jin H., Lee H., Heo J., et al. Senescence-Associated MCP-1 Secretion Is Dependent on a Decline in BMI1 in Human Mesenchymal Stromal Cells. Antioxid Redox Signal. 2016 Mar 20;24(9):471-85. doi: 10.1089/ars.2015.6359.
    Species: Human
    Sample type: Conditioned Media
  4. Longhin E., Capasso L., Battaglia C., et al. Integrative transcriptomic and protein analysis of human bronchial BEAS-2B exposed to seasonal urban particulate matter. Environmental Pollution Volume 209, February 2016, Pages 87-98
    Species: Human
    Sample type: Cell Lysate
  5. Lee J., Schierer S., Blume K., et al. HIV-Nef and ADAM17-Containing Plasma Extracellular Vesicles Induce and Correlate with Immune Pathogenesis in Chronic HIV Infection. EBioMedicine. 2016 Apr; 6: 103-113.
    Species: Human
    Sample type: Extracellular Vesicles
  6. Blauth K., Zhang X., Chopra M., et al. The role of fractalkine (CX3CL1) in regulation of CD4+ cell migration to the central nervous system in patients with relapsing-remitting multiple sclerosis. Clinical Immunology, Available online 14 January 2015, doi:10.1016/j.clim.2015.01.001
    Species: Human
    Sample type: CSF
  7. Lejmi E., Perriraz N., Clement S., et al. Inflammatory Chemokines MIP-1d and MIP-3a Are Involved in the Migration of Multipotent Mesenchymal Stromal Cells Induced by Hepatoma Cells.
    Species: Human
    Sample type: Conditioned Media
  8. Loria F., Diaz-Nido J. Frataxin knockdown in human astrocytes triggers cell death and the release of factors that cause neuronal toxicity. Neurobiology of Disease, 76 (2015) 1-12. doi:10.1016/j.nbd.2014.12.017
    Species: Human
    Sample type: Conditioned Media
  9. Moore MC., Pandolfi V., McFetridge P. Novel human-derived extracellular matrix induces in vitro and in vivo vascularization and inhibits fibrosis. Biomaterials. 2015 May;49:37-46. doi: 10.1016/j.biomaterials.2015.01.022.
    Species: Human
    Sample type: Conditioned Media
  10. Zhang L., Lio D., Wang Y., et al. The role of Toll-like receptor 3 and 4 in regulating the function of mesenchymal stem cells isolated from umbilical cord. Int J Mol Med. 2015 Apr;35(4):1003-10. doi: 10.3892/ijmm.2015.2106.
    Species: Human
    Sample type: Conditioned Media

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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