Human Cytokine Array C8

RayBio® C-Series Human Cytokine Antibody Array 8 Kit. Detects 54 Human Cytokines. Suitable for all liquid sample types.

$440.36
AAH-CYT-8-2
+

Antigen Information

Gene Symbols:
ALCAM | BMP5 | BMP7 | CCL23 | CD14 | CD80 | CDH5 | CSF1R | CTF1 | CXCL10   View more

Assay Format

Number of Targets Detected:
54
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Activin A
ALCAM
(CD166)
CD80
(B7-1)
BMP-5
BMP-7
Cardiotrophin-1
(CT-1)
CD14
CXCL16
DR6
(TNFRSF21)
Endoglin
(CD105)
ErbB3
E-Selectin
Fas Ligand
(TNFSF6)
ICAM-2
(CD102)
IGF-2
  • Gene Symbol: Igf2
  • Gene ID: 3481
  • Accession #: P01344
IL-1 R2
IL-10 R beta
IL-13 R alpha 2
IL-18 BP alpha
IL-18 R beta
(AcPL)
MMP-3
IL-2 R beta
(CD122)
IL-2 R gamma
(Common gamma Chain)
  • Gene Symbol: IL2RG
  • Gene ID: 3561
  • Accession #: P31785
IL-21 R
IL-5 R alpha
IL-9
IP-10
(CXCL10)
LAP/TGF beta 1
  • Gene Symbol: TGFB1
  • Gene ID: 7040
  • Accession #: P01137
Leptin R
  • Gene Symbol: LEPR
  • Gene ID: 3953
  • Accession #: P48357
LIF
L-Selectin
(CD62L)
M-CSF R
MMP-1
MMP-13
MMP-9
MPIF-1
(CCL23)
NGFR
(TNFRSF16)
PDGF-AA
PDGF-AB
PDGF R alpha
PDGF R beta
PECAM-1
(CD31)
Prolactin
  • Gene Symbol: PRL
  • Gene ID: 5617
  • Accession #: P01236
SCF R
(CD117/c-kit)
SDF-1 beta
(CXCL12 beta)
Siglec-5
(CD170)
TGF alpha
TGF beta 2
Tie-1
Tie-2
TIMP-4
VE-Cadherin
(CDH5)
  • Gene Symbol: CDH5
  • Gene ID: 1003
  • Accession #: P33151
VEGFR2
VEGFR3

Species Detected

Human

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Cytokine Antibody Array C8 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Shang T., Yao B., Gao D., et al. A novel model of humanised keloid scarring in mice. Int Wound J. 2018 Feb;15(1):90-94. doi: 10.1111/iwj.12838.
    Species: Human
    Sample type: Conditioned Media
  2. Li MF., Zhang R., Li TT., et al. High Glucose Increases the Expression of Inflammatory Cytokine Genes in Macrophages Through H3K9 Methyltransferase Mechanism. J Interferon Cytokine Res. 2016 Jan;36(1):48-61. doi: 10.1089/jir.2014.0172
    Species: Human
    Sample type: Conditioned Media
  3. Jin H., Lee H., Heo J., et al. Senescence-Associated MCP-1 Secretion Is Dependent on a Decline in BMI1 in Human Mesenchymal Stromal Cells. Antioxid Redox Signal. 2016 Mar 20;24(9):471-85. doi: 10.1089/ars.2015.6359.
    Species: Human
    Sample type: Conditioned Media
  4. Silva G., Aboussekhra A. p16INK4A Inhibits the Pro-Metastatic Potentials of Osteosarcoma Cells Through Targeting the ERK Pathway and TGF-b1. Mol Carcinog. 2015 Mar 1. doi: 10.1002/mc.22299.
    Species: Human
    Sample type: Conditioned Media
  5. Jin HJ., Lee HJ., Heo J., et al. Senescence associated MCP-1 secretion is dependent on a decline in BMI1 in human mesenchymal stromal cells. Antioxidants & redox signalling [1523-0864], 2015, epub ahead of print.
    Species: Human
    Sample type: Conditioned Media
  6. Barr P., Li H., Spier C., et al. Phase II Intergroup Trial of Alisertib in Relapsed and Refractory Peripheral T-Cell Lymphoma and Transformed Mycosis Fungoides: SWOG 1108. J Clin Oncol. 2015 Jul 20;33(21):2399-404. doi: 10.1200/JCO.2014.60.6327.
    Species: Human
    Sample type: Serum
  7. Anderson J., et al. Phosphoproteomic Profiling Reveals IL6-mediated Paracrine Signaling within the Ewing Sarcoma Family of Tumors. Mol Cancer Res., Published Online First August 4, 2014; doi: 10.1158/1541-7786.MCR-14-0159
    Species: Human
    Sample type: Conditioned Media
  8. Chen W., Ho C., Chang Y., Chen H., Lin C., et al. Cancer-associated fibroblasts regulate the plasticity of lung cancer stemness via paracrine signalling. Nat. Comm. 2014, 5, article 3472. doi:10.1038/ncomms4472
    Species: Human
    Sample type: Conditioned Media
  9. Le Bras G., et al. TGF? loss activates ADAMTS-1-mediated EGF-dependent invasion in a model of esophageal cell invasion. Experimental Cell Research Available online 23 July 2014. DOI: 10.1016/j.yexcr.2014.07.021
    Species: Human
    Sample type: Conditioned Media
  10. Ho Ia., Toh H., Ng W., et al. Human bone marrow-derived mesenchymal stem cells suppress human glioma growth through inhibition of angiogenesis. Stem Cells. 2013 Jan;31(1):146-55. doi: 10.1002/stem.1247
    Species: Human
    Sample type: Conditioned Media

No posts found

Write A Review
U.S. Non-U.S.

Analysis Tool Bundle Discount

Specify Size
1 x $440.36
+
1 x $116.15 $34.84

70% off the data analysis tool when you purchase the array and tool together!


Note: If you are not placing this order online, make sure to mention this bundle discount to our orders department (orders@raybiotech.com).

Total list price $556.51
Price for all $475.20

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

Amazon Gift Card Program

C-Series Testing Service C-Series Testing Service

Related Products Related Products