Human Cytokine Array C9

RayBio® C-Series Human Cytokine Antibody Array 9 Kit. Detects 51 Human Cytokines. Suitable for all liquid sample types.

$650.44
AAH-CYT-9-2
+

Antigen Information

Gene Symbols:
ADAM17 | AGER | BCAM | CD40 | CFD | CGA | EDA2R | FCGR2B | FST | FSTL3   View more

Assay Format

Number of Targets Detected:
51
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Adipsin
(Complement Factor D)
BCAM
CD30
(TNFRSF8)
CD40
(TNFRSF5)
Fc gamma RIIB/C
(CD32B/C)
Ferritin
FLRG
Follistatin
Furin
Galectin-7
GDF-15
Growth Hormone
IL-10 R alpha
IL-22
IL-28A
(IFN-lambda 2)
IL-29
(IFN-lambda 1)
IL-31
Insulin
Luteinizing hormone
LIMPII
LYVE-1
Marapsin
MICA
MICB
MMP-2
MMP-7
MMP-8
MMP-10
NCAM-1
(CD56)
Nidogen-1
NrCAM
NRG1-beta 1
(HRG1-beta 1)
Osteopontin
(SPP1)
PAI-1
Platelet factor 4
(CXCL4)
PSA-total
RAGE
RANK
(TNFRSF11A)
Resistin
SAA
(Serum Amyloid A)
Siglec-9
TACE
TIM-1
(KIM-1)
TRAIL R2
(TNFRSF10B/DR5)
Trappin-2
TREM-1
TSH
TSLP
VCAM-1
(CD106)
VEGF-C
XEDAR
  • Gene Symbol: EDA2R
  • Gene ID: 60401
  • Accession #: Q9HAV5

Species Detected

Human

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Cytokine Antibody Array C9 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Silva G., Aboussekhra A. p16INK4A Inhibits the Pro-Metastatic Potentials of Osteosarcoma Cells Through Targeting the ERK Pathway and TGF-b1. Mol Carcinog. 2015 Mar 1. doi: 10.1002/mc.22299.
    Species: Human
    Sample type: Conditioned Media
  2. Kim D., Lee D., Chang E., et al. GDF-15 secreted from human umbilical cord blood mesenchymal stem cells delivered through the cerebrospinal fluid promotes hippocampal neurogenesis and synaptic activity in an Alzheimer's disease model. Stem Cells Dev. 2015 Oct 15;24(20):2378-90. doi: 10.1089/scd.2014.0487.
    Species: Human
    Sample type: Conditioned Media
  3. Chen W., Ho C., Chang Y., Chen H., Lin C., et al. Cancer-associated fibroblasts regulate the plasticity of lung cancer stemness via paracrine signalling. Nat. Comm. 2014, 5, article 3472. doi:10.1038/ncomms4472
    Species: Human
    Sample type: Conditioned Media
  4. Raschke, S., Eckardt, K., Holven, K. B., Jensen, J., & Eckel, J. (2013). Identification and validation of novel contraction-regulated myokines released from primary human skeletal muscle cells. PloS one, 8(4), e62008.
    Species: Human
    Sample type: Conditioned Media
  5. Sofrenovic T., McEwan K., Crowe S., et al. Circulating angiogenic cells can be derived from cryopreserved peripheral blood mononuclear cells. PLoS One. 2012;7(10):e48067. doi: 10.1371/journal.pone.0048067
    Species: Human
    Sample type: Conditioned Media
  6. Kim M, Oskarsson T, Acharyya S, Nguyen D, Zhang X, et al.Tumor Self-Seeding by Circulating Cancer Cells. Cell. 2009;139(7):1315-1326.
    Species: Human
    Sample type: Conditioned Media

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Total list price $766.59
Price for all $685.28

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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