Human Cytokine Array C1000

RayBio® C-Series Human Cytokine Antibody Array 1000 Kit. A combination of Human Cytokine Antibody Array C6 & C7. Detects 120 Human Cytokines. Suitable for all liquid sample types.

$650.44  
AAH-CYT-1000-2
+

Antigen Information

Gene Symbols:
ADIPOQ | AGRP | ANG | ANGPT2 | AREG | AXL | BDNF | BMP4 | BMP6 | BTC   View more

Assay Format

Number of Targets Detected:
120
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Adiponectin
(ACRP30)
AgRP
Amphiregulin
Angiogenin
Angiopoietin-2
Axl
BDNF
bFGF
BLC
(CXCL13)
BMP-4
BMP-6
beta-NGF
Betacellulin
(BTC)
CCL28
(MEC)
CK beta 8-1
(CCL23)
CNTF
CTACK
(CCL27)
Dtk
EGF
EGFR
ENA-78
(CXCL5)
Eotaxin-1
(CCL11)
Eotaxin-2
(MPIF-2/CCL24)
Eotaxin-3
(CCL26)
Fas
(TNFRSF6/Apo-1)
FGF-4
FGF-6
FGF-7
(KGF)
FGF-9
Flt-3 Ligand
Fractalkine
(CX3CL1)
GCP-2
(CXCL6)
GCSF
GDNF
GITR
(TNFRSF18)
GITR Ligand
(TNFSF18)
GM-CSF
GRO alpha/beta/gamma
GRO alpha
(CXCL1)
HCC-4
(CCL16)
HGF
I-309
(TCA-3/CCL1)
ICAM-1
(CD54)
ICAM-3
(CD50)
IFN-gamma
IGFBP-1
IGFBP-2
IGFBP-3
IGFBP-4
IGFBP-6
IGF-1
IGF-1 R
IL-1 R4
(ST2)
IL-1 R1
IL-10
IL-11
IL-12 p40
IL-12 p70
IL-13
IL-15
IL-16
IL-17A
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-1 ra
(IL-1 F3)
IL-2
IL-2 R alpha
IL-3
IL-4
IL-5
IL-6
IL-6 R
IL-7
IL-8
(CXCL8)
I-TAC
(CXCL11)
Leptin
Light
(TNFSF14)
Lymphotactin
(XCL1)
MCP-1
(CCL2)
MCP-2
(CCL8)
MCP-3
(MARC/CCL7)
MCP-4
(CCL13)
M-CSF
MDC
(CCL22)
MIF
MIG
(CXCL9)
MIP-1 alpha
(CCL3)
MIP-1 beta
(CCL4)
MIP-1 delta
(CCL15)
MIP-3 alpha
(CCL20)
MIP-3 beta
(CCL19)
MSP alpha/beta
NAP-2
(PPBP/CXCL7)
NT-3
NT-4
Oncostatin M
Osteoprotegerin
(TNFRSF11B)
PARC
(CCL18)
PDGF-BB
PLGF
RANTES
(CCL5)
SCF
SDF-1 alpha
(CXCL12 alpha)
gp130
TNF RII
(TNFRSF1B)
TNF RI
(TNFRSF1A)
TARC
(CCL17)
TECK
(CCL25)
TGF beta 3
  • Gene Symbol: TGFB3
  • Gene ID: 7043
  • Accession #: P10600
TGF beta 1
Thrombopoietin
(TPO)
TIMP-1
TIMP-2
TNF alpha
TNF beta
(TNFSF1B)
TRAIL R3
(TNFRSF10C)
TRAIL R4
(TNFRSF10D)
uPAR
VEGF-A
VEGF-D

Species Detected

Human

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Cytokine Antibody Array C1000 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Boucek RJ., et al. < i> Ex Vivo Paracrine Properties of Cardiac Tissue: Effects of Chronic Heart Failure. J of Heart and Lung Transpl. Available online 17 July 2014 DOI: 10.1016/j.healun.2014.07.010
    Species: Human
    Sample type: Conditioned Media
  2. Chen YY., Chang J., Huang W., Hsu S., Kuo S., Sun J., Dou H. The Pattern of cytokine production in vitro induced by ancient and moden mycobacterium tuberculosis strains. PLOS One Published: April 11, 2014 DOI: 10.1371/journal.pone.0094296
    Species: Human
    Sample type: Conditioned Media
  3. Chung E., et al. CXCL5 abundant in the wound fluid at the late phase of wound healing, possibly promoting migration of mesenchymal stem cells and vascular tube formation. Tissue Engineering and Regenerative Medicine August 2014, Volume 11, Issue 4, pp 317-322
    Species: Human
    Sample type: Wound Fluid
  4. Jung JH., Lee SJ., Kim JH., et al. Chemokine (C-X-C Motif) Receptor 2 and Its Related Ligands Play a Novel Role in Supporting the Pluripotency and Proliferation of Human Pluripotent Stem Cells. Stem Cells Dev. 2014 Nov 12. [Epub ahead of print]: DOI: 10.1089/scd.2014.0381
    Species: Human
    Sample type: Conditioned Media
  5. Kavitha, C. V., Deep, G., Gangar, S. C., Jain, A. K., Agarwal, C. and Agarwal, R. (2014), Silibinin inhibits prostate cancer cells- and RANKL-induced osteoclastogenesis by targeting NFATc1, NF-?B, and AP-1 activation in RAW264.7 cells. Mol. Carcinog., 53: 169-180. doi: 10.1002/mc.21959
    Species: Mouse
    Sample type: Conditioned Media
  6. Mannoji C., Koda M., Kamya K., et al. Transplantation of human bone marrow stromal cell-derived neuroregenrative cells promotes functional recovery after spinal cord injury in mice. Acta Neurobiol Exp. 2014;74(4):479-88
    Species: Human
    Sample type: Conditioned Media
  7. Pu D., Wang W. Toll?like receptor 4 agonist, lipopolysaccharide, increases the expression levels of cytokines and chemokines in human peripheral blood mononuclear cells. EXPERIMENTAL AND THERAPEUTIC MEDICINE 8: 1914-1918, 2014. DOI: 10.3892/etm.2014.2025
    Species: Human
    Sample type: Conditioned Media
  8. Shih Y., Wang M., Zhou J., Peng H., Lee D., Chiu J. Endothelial progenitors promote hepatocarcinoma intrahepatic metastasis through monocyte chemotactic protein-1 induction of microRNA-21. Gut 2014 Epub ahead of print June 17 2014, doi:10.1136/gutjnl-2013-306302
    Species: Human
    Sample type: Cell Lysate
  9. Gromov P., et al. Tumor interstitial fluid — A treasure trove of cancer biomarkers. Biochimica et Biophysica Acta 1834 (2013) 2259-2270. http://dx.doi.org/10.1016/j.bbapap.2013.01.013
    Species: Human
    Sample type: Interstitial Fluid, Tumor
  10. Lin Y., et al. A panel of four cytokines predicts the prognosis of patients with malignant gliomas. J Neurooncol (2013) 114:199-208, DOI 10.1007/s11060-013-1171-x
    Species: Human
    Sample type: Plasma
Easy, reliable and replicable

Penn State
This is a very trustworthy product.

University of Southern California
I have been using these arrays and having pretty good results. My different treatments up and down regulate the expression of these cytokines, which can be clearly seen on the membrane spots.

Florida A & M University
I used this product for testing tumor tissues protein lysates to compare cytokine between control and treated conditions. The results so far has been satisfactory
Write A Review

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

C-Series Testing Service C-Series Testing Service

Amazon Gift Card Program

Customers Also Bought Customers Also Bought