Human Cytokine Array C1000

$644.00
AAH-CYT-1000-2

RayBio® C-Series Human Cytokine Antibody Array 1000 Kit. A combination of Human Cytokine Antibody Array C6 & C7. Detects 120 Human Cytokines. Suitable for all liquid sample types.

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Antigen Information

Gene Symbol:
ADIPOQ | AGRP | ANG | ANGPT2 | AREG | AXL | BDNF | BMP4 | BMP6 | BTC   View more

Assay Format

Number of Targets Detected:
120
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Adiponectin
(ACRP30)
AgRP
Amphiregulin
Angiogenin
Angiopoietin-2
Axl
BDNF
bFGF
BLC
(CXCL13)
BMP-4
BMP-6
beta-NGF
Betacellulin
(BTC)
CCL28
(MEC)
CK beta 8-1
(CCL23)
CNTF
CTACK
(CCL27)
Dtk
EGF
EGFR
ENA-78
(CXCL5)
Eotaxin-1
(CCL11)
Eotaxin-2
(MPIF-2/CCL24)
Eotaxin-3
(CCL26)
Fas
(TNFRSF6/Apo-1)
FGF-4
FGF-6
FGF-7
(KGF)
FGF-9
Flt-3 Ligand
Fractalkine
(CX3CL1)
GCP-2
(CXCL6)
GCSF
GDNF
GITR
(TNFRSF18)
GITR Ligand
(TNFSF18)
GM-CSF
GRO alpha/beta/gamma
GRO alpha
(CXCL1)
HCC-4
(CCL16)
HGF
I-309
(TCA-3/CCL1)
ICAM-1
(CD54)
ICAM-3
(CD50)
IFN-gamma
IGFBP-1
IGFBP-2
IGFBP-3
IGFBP-4
IGFBP-6
IGF-1
IGF-1 R
IL-1 R4
(ST2)
IL-1 R1
IL-10
IL-11
IL-12 p40
IL-12 p70
IL-13
IL-15
IL-16
IL-17A
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-1 ra
(IL-1 F3)
IL-2
IL-2 R alpha
IL-3
IL-4
IL-5
IL-6
IL-6 R
IL-7
IL-8
(CXCL8)
I-TAC
(CXCL11)
Leptin
Light
(TNFSF14)
Lymphotactin
(XCL1)
MCP-1
(CCL2)
MCP-2
(CCL8)
MCP-3
(MARC/CCL7)
MCP-4
(CCL13)
M-CSF
MDC
(CCL22)
MIF
MIG
(CXCL9)
MIP-1 alpha
(CCL3)
MIP-1 beta
(CCL4)
MIP-1 delta
(CCL15)
MIP-3 alpha
(CCL20)
MIP-3 beta
(CCL19)
MSP alpha/beta
NAP-2
(PPBP/CXCL7)
NT-3
NT-4
Oncostatin M
Osteoprotegerin
(TNFRSF11B)
PARC
(CCL18)
PDGF-BB
PLGF
RANTES
(CCL5)
SCF
SDF-1 alpha
(CXCL12 alpha)
gp130
TNF RII
(TNFRSF1B)
TNF RI
(TNFRSF1A)
TARC
(CCL17)
TECK
(CCL25)
TGF beta 3
  • Gene Symbol: TGFB3
  • Gene ID: 7043
  • Accession #: P10600
TGF beta 1
Thrombopoietin
(TPO)
TIMP-1
TIMP-2
TNF alpha
TNF beta
(TNFSF1B)
TRAIL R3
(TNFRSF10C)
TRAIL R4
(TNFRSF10D)
uPAR
VEGF-A
VEGF-D

Species Detected

Human

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Cytokine Antibody Array C1000 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Longhin E., Capasso L., Battaglia C., et al. Integrative transcriptomic and protein analysis of human bronchial BEAS-2B exposed to seasonal urban particulate matter. Environmental Pollution Volume 209, February 2016, Pages 87-98
    Species: Human
    Sample type: Cell Lysate
  2. Lee J., Schierer S., Blume K., et al. HIV-Nef and ADAM17-Containing Plasma Extracellular Vesicles Induce and Correlate with Immune Pathogenesis in Chronic HIV Infection. EBioMedicine. 2016 Apr; 6: 103-113.
    Species: Human
    Sample type: Extracellular Vesicles
  3. Blauth K., Zhang X., Chopra M., et al. The role of fractalkine (CX3CL1) in regulation of CD4+ cell migration to the central nervous system in patients with relapsing-remitting multiple sclerosis. Clinical Immunology, Available online 14 January 2015, doi:10.1016/j.clim.2015.01.001
    Species: Human
    Sample type: CSF
  4. Lejmi E., Perriraz N., Clement S., et al. Inflammatory Chemokines MIP-1d and MIP-3a Are Involved in the Migration of Multipotent Mesenchymal Stromal Cells Induced by Hepatoma Cells.
    Species: Human
    Sample type: Conditioned Media
  5. Loria F., Diaz-Nido J. Frataxin knockdown in human astrocytes triggers cell death and the release of factors that cause neuronal toxicity. Neurobiology of Disease, 76 (2015) 1-12. doi:10.1016/j.nbd.2014.12.017
    Species: Human
    Sample type: Conditioned Media
  6. Moore MC., Pandolfi V., McFetridge P. Novel human-derived extracellular matrix induces in vitro and in vivo vascularization and inhibits fibrosis. Biomaterials. 2015 May;49:37-46. doi: 10.1016/j.biomaterials.2015.01.022.
    Species: Human
    Sample type: Conditioned Media
  7. Zhang L., Lio D., Wang Y., et al. The role of Toll-like receptor 3 and 4 in regulating the function of mesenchymal stem cells isolated from umbilical cord. Int J Mol Med. 2015 Apr;35(4):1003-10. doi: 10.3892/ijmm.2015.2106.
    Species: Human
    Sample type: Conditioned Media
  8. McLane J., Ligon L. Palladin mediates stiffness-induced fibroblast activation in the tumor microenvironment. Biophys J. 2015 Jul 21;109(2):249-64. doi: 10.1016/j.bpj.2015.06.033.
    Species: Human
    Sample type: Conditioned Media
  9. Yang Y., Wang Y., Li L., et al. Toll?like receptor 9 agonist stimulation enables osteogenic differentiation without altering the immune status of umbilical cord mesenchymal stem cells. Mol Med Rep. 2015 Dec;12(6):8077-84. doi: 10.3892/mmr.2015.4429.
    Species: Human
    Sample type: Conditioned Media
  10. Wee Z., Yatim S., Kohlbauer V., et al. IRAK1 is a therapeutic target that drives breast cancer metastasis and resistance to paclitaxel. Nat Commun. 2015 Oct 27;6:8746. doi: 10.1038/ncomms9746.
    Species: Human
    Sample type: Conditioned Media
I have been using these arrays and having pretty good results. My different treatments up and down regulate the expression of these cytokines, which can be clearly seen on the membrane spots.
I used this product for testing tumor tissues protein lysates to compare cytokine between control and treated conditions. The results so far has been satisfactory
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Note: If you are not placing this order online, make sure to mention this bundle discount to our orders department (orders@raybiotech.com).

Total list price $818.00
Price for all $696.20

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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