Human Cytokine Array C1000

RayBio® C-Series Human Cytokine Antibody Array 1000 Kit. A combination of Human Cytokine Antibody Array C6 & C7. Detects 120 Human Cytokines. Suitable for all liquid sample types.

$650.44  
AAH-CYT-1000-2
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Antigen Information

Gene Symbols:
ADIPOQ | AGRP | ANG | ANGPT2 | AREG | AXL | BDNF | BMP4 | BMP6 | BTC   View more

Assay Format

Number of Targets Detected:
120
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Adiponectin
(ACRP30)
AgRP
Amphiregulin
Angiogenin
Angiopoietin-2
Axl
BDNF
bFGF
BLC
(CXCL13)
BMP-4
BMP-6
beta-NGF
Betacellulin
(BTC)
CCL28
(MEC)
CK beta 8-1
(CCL23)
CNTF
CTACK
(CCL27)
Dtk
EGF
EGFR
ENA-78
(CXCL5)
Eotaxin-1
(CCL11)
Eotaxin-2
(MPIF-2/CCL24)
Eotaxin-3
(CCL26)
Fas
(TNFRSF6/Apo-1)
FGF-4
FGF-6
FGF-7
(KGF)
FGF-9
Flt-3 Ligand
Fractalkine
(CX3CL1)
GCP-2
(CXCL6)
GCSF
GDNF
GITR
(TNFRSF18)
GITR Ligand
(TNFSF18)
GM-CSF
GRO alpha/beta/gamma
GRO alpha
(CXCL1)
HCC-4
(CCL16)
HGF
I-309
(TCA-3/CCL1)
ICAM-1
(CD54)
ICAM-3
(CD50)
IFN-gamma
IGFBP-1
IGFBP-2
IGFBP-3
IGFBP-4
IGFBP-6
IGF-1
IGF-1 R
IL-1 R4
(ST2)
IL-1 R1
IL-10
IL-11
IL-12 p40
IL-12 p70
IL-13
IL-15
IL-16
IL-17A
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-1 ra
(IL-1 F3)
IL-2
IL-2 R alpha
IL-3
IL-4
IL-5
IL-6
IL-6 R
IL-7
IL-8
(CXCL8)
I-TAC
(CXCL11)
Leptin
Light
(TNFSF14)
Lymphotactin
(XCL1)
MCP-1
(CCL2)
MCP-2
(CCL8)
MCP-3
(MARC/CCL7)
MCP-4
(CCL13)
M-CSF
MDC
(CCL22)
MIF
MIG
(CXCL9)
MIP-1 alpha
(CCL3)
MIP-1 beta
(CCL4)
MIP-1 delta
(CCL15)
MIP-3 alpha
(CCL20)
MIP-3 beta
(CCL19)
MSP alpha/beta
NAP-2
(PPBP/CXCL7)
NT-3
NT-4
Oncostatin M
Osteoprotegerin
(TNFRSF11B)
PARC
(CCL18)
PDGF-BB
PLGF
RANTES
(CCL5)
SCF
SDF-1 alpha
(CXCL12 alpha)
gp130
TNF RII
(TNFRSF1B)
TNF RI
(TNFRSF1A)
TARC
(CCL17)
TECK
(CCL25)
TGF beta 3
  • Gene Symbol: TGFB3
  • Gene ID: 7043
  • Accession #: P10600
TGF beta 1
Thrombopoietin
(TPO)
TIMP-1
TIMP-2
TNF alpha
TNF beta
(TNFSF1B)
TRAIL R3
(TNFRSF10C)
TRAIL R4
(TNFRSF10D)
uPAR
VEGF-A
VEGF-D

Species Detected

Human

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Cytokine Antibody Array C1000 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Kim M, Oskarsson T, Acharyya S, Nguyen D, Zhang X, et al.Tumor Self-Seeding by Circulating Cancer Cells. Cell. 2009;139(7):1315-1326.
    Species: Human
    Sample type: Conditioned Media (Cell-conditioned media)
  2. Ray S, Britschgi M, Herbert C, Takeda-Uchimura Y, et al. Classification and prediction of clinical Alzheimer's diagnosis based on plasma signaling proteins. Nat Med. 2007; 13(11):1359-1362.
    Species: Human
    Sample type: Plasma (Plasma)
  3. Osman L, Yacoub MH, Latif N, Amrani M, Chester AH. Role of Human Valve Interstitial Cells in Valve Calcification and Their Response to Atorvastatin. Circulation. 2006;114(suppl.1):I547-I552.
    Species: Human
    Sample type: Conditioned Media (Aortic valve interstitial cell-cultured medium)
  4. Wiley C., Velarde M., Lecot P., et al. Mitochondrial Dysfunction Induces Senescence with a Distinct Secretory Phenotype. Cell Metab . 2016 Feb 9;23(2):303-14. doi: 10.1016/j.cmet.2015.11.011. Epub 2015 Dec 10.
    Species: Human
    Sample type: Conditioned Media (IMR-90 cells, SIRT3-depletion effect on secretion)
  5. Giroux M, Denis F. Human CD1d-unrestricted NKT cells release chemokines upon Fas engagement. Blood. 2005;105(2):703-710.
    Species: Human
    Sample type: Conditioned Media (CD56+CD3+ cell-conditioned media)
  6. Giroux, M et al. CD1d-unrestricted human NKT cells release chemokines upon Fas engagement. CD1d-unrestricted human NKT cells release chemokines upon Fas engagement. CD1d-unrestricted human NKT cells release chemokines upon Fas engagement. Blood Volume 105, Issue 2, Pages 703-10, Jan 2005
    Species: Human
    Sample type: Conditioned Media (membrane)
  7. Wang Y, Cai D, Brendel C, Barett C, Erben P, et al. Adaptive secretion of granulocyte-macrophage colony-stimulating factor (GM-CSF) mediates imatinib and nilotinib resistance in BCR/ABL+ progenitors via JAK-1/STAT-5 pathway activation. Blood. 2007;109:2147-2155.
    Species: Human
    Sample type: Conditioned Media (BCR/ABL+ leukemia (LAMA 25R or 25UR) cell-conditioned media)
  8. Lotti, Fiorenza, et al. "Chemotherapy activates cancer-associated fibroblasts to maintain colorectal cancer-initiating cells by IL-17A." Journal of Experimental Medicine 210.13 (2013): 2851-2872.
    Species: Human
    Sample type: Conditioned Media ( CAFs or CICs)
  9. Shih Y., Wang M., Zhou J., Peng H., Lee D., Chiu J. Endothelial progenitors promote hepatocarcinoma intrahepatic metastasis through monocyte chemotactic protein-1 induction of microRNA-21. Gut 2014 Epub ahead of print June 17 2014, doi:10.1136/gutjnl-2013-306302
    Species: Human
    Sample type: Cell Lysate (Colony Foruming Unit Endothelial Cells)
  10. Wee Z., Yatim S., Kohlbauer V., et al. IRAK1 is a therapeutic target that drives breast cancer metastasis and resistance to paclitaxel. Nat Commun. 2015 Oct 27;6:8746. doi: 10.1038/ncomms9746.
    Species: Human
    Sample type: Conditioned Media (Mammosphere breast cancer model)
I used this array for the second time to study the effect of my compound of different cytokine expression. I published the paper recently:

Messeha Messeha SS, Zarmouh NO, Mendonca P, Carolyn Cotton, Karam F A Soliman: Molecular mechanism of gossypol mediating CCL2 and IL‑8 attenuation in triple‑negative breast cancer cells. Molecular medicine reports 22: 1213-1226, 2020.

Florida A & M University, Tallahassee, Florida
I trust the product from RayBiotech. It shows accurate results. I published the following paper using the human cytokine array:

Messeha, S.S., Zarmouh, N.O., Mendonca, P., Alwagdani, H., Kolta, M.G., Soliman, K.F.A., 2018. The inhibitory effects of plumbagin on the NF-B pathway and CCL2 release in racially different triple-negative breast cancer cells. PloS one 13, e0201116.

Florida A & M University, Tallahassee, Florida
Easy, reliable and replicable

Penn State
This is a very trustworthy product.

University of Southern California
I have been using these arrays and having pretty good results. My different treatments up and down regulate the expression of these cytokines, which can be clearly seen on the membrane spots.

Florida A & M University
I used this product for testing tumor tissues protein lysates to compare cytokine between control and treated conditions. The results so far has been satisfactory
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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