Human Cytokine Array C2000

RayBio® C-Series Human Cytokine Antibody Array 2000 Kit. A combination of Human Cytokine Antibody Array C6, C7, & C8. Detects 174 Human Cytokines. Suitable for all liquid sample types.

$1,314.01
AAH-CYT-2000-2
+

Antigen Information

Gene Symbols:
ADIPOQ | AGRP | ALCAM | ANG | ANGPT2 | AREG | AXL | BDNF | BMP4 | BMP5   View more

Assay Format

Number of Targets Detected:
120
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Adiponectin
(ACRP30)
Activin A
AgRP
ALCAM
(CD166)
Amphiregulin
Angiogenin
Angiopoietin-2
Axl
CD80
(B7-1)
BDNF
bFGF
BLC
(CXCL13)
BMP-4
BMP-5
BMP-6
BMP-7
beta-NGF
Betacellulin
(BTC)
Cardiotrophin-1
(CT-1)
CCL28
(MEC)
CD14
Ck beta 8-1
(CCL23)
CNTF
CTACK
(CCL27)
CXCL16
DR6
(TNFRSF21)
Dtk
EGF
EGFR
ENA-78
(CXCL5)
Endoglin
(CD105)
Eotaxin-1
(CCL11)
Eotaxin-2
(MPIF-2/CCL24)
Eotaxin-3
(CCL26)
ErbB3
E-Selectin
Fas
(TNFRSF6/Apo-1)
Fas Ligand
(TNFSF6)
FGF-4
FGF-6
FGF-7
(KGF)
FGF-9
Flt-3 Ligand
Fractalkine
(CX3CL1)
GCP-2
(CXCL6)
GCSF
GDNF
GITR
(TNFRSF18)
GITR Ligand
(TNFSF18)
GM-CSF
GRO alpha/beta/gamma
GRO alpha
(CXCL1)
HCC-4
(CCL16)
HGF
I-309
(TCA-3/CCL1)
ICAM-1
(CD54)
ICAM-2
(CD102)
ICAM-3
(CD50)
IFN-gamma
IGFBP-1
IGFBP-2
IGFBP-3
IGFBP-4
IGFBP-6
IGF-1
IGF-1 R
IGF-2
  • Gene Symbol: Igf2
  • Gene ID: 3481
  • Accession #: P01344
IL-1 R2
IL-1 R4
(ST2)
IL-1 R1
IL-10
IL-10 R beta
IL-11
IL-12 p40
IL-12 p70
IL-13
IL-13 R alpha 2
IL-15
IL-16
IL-17A
  • Gene Symbol: IL17A
  • Gene ID: 3605
  • Accession #: Q16552
IL-18 BP alpha
IL-18 R beta
(AcPL)
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-1 ra
(IL-1 F3)
IL-2
IL-2 R beta
(CD122)
IL-2 R gamma
(Common gamma Chain)
  • Gene Symbol: IL2RG
  • Gene ID: 3561
  • Accession #: P31785
IL-2 R alpha
IL-21 R
IL-3
IL-4
IL-5
IL-5 R alpha
IL-6
IL-6 R
IL-7
IL-8
(CXCL8)
IL-9
IP-10
(CXCL10)
I-TAC
(CXCL11)
LAP/TGF beta 1
  • Gene Symbol: TGFB1
  • Gene ID: 7040
  • Accession #: P01137
Leptin
Leptin R
  • Gene Symbol: LEPR
  • Gene ID: 3953
  • Accession #: P48357
LIF
Light
(TNFSF14)
L-Selectin
(CD62L)
Lymphotactin
(XCL1)
MCP-1
(CCL2)
MCP-2
(CCL8)
MCP-3
(MARC/CCL7)
MCP-4
(CCL13)
M-CSF
M-CSF R
MDC
(CCL22)
MIF
MIG
(CXCL9)
MIP-1 alpha
(CCL3)
MIP-1 beta
(CCL4)
MIP-1 delta
(CCL15)
MIP-3 alpha
(CCL20)
MIP-3 beta
(CCL19)
MMP-1
MMP-13
MMP-3
MMP-9
MPIF-1
(CCL23)
MSP alpha/beta
NAP-2
(PPBP/CXCL7)
NGFR
(TNFRSF16)
NT-3
NT-4
Oncostatin M
Osteoprotegerin
(TNFRSF11B)
PARC
(CCL18)
PDGF-AA
PDGF R alpha
PDGF R beta
PDGF-AB
PDGF-BB
PECAM-1
(CD31)
PLGF
Prolactin
  • Gene Symbol: PRL
  • Gene ID: 5617
  • Accession #: P01236
RANTES
(CCL5)
SCF
SCF R
(CD117/c-kit)
SDF-1 alpha
(CXCL12 alpha)
SDF-1 beta
(CXCL12 beta)
gp130
Siglec-5
(CD170)
TNF RII
(TNFRSF1B)
TNF RI
(TNFRSF1A)
TARC
(CCL17)
TECK
(CCL25)
TGF beta 2
TGF alpha
TGF beta 3
  • Gene Symbol: TGFB3
  • Gene ID: 7043
  • Accession #: P10600
TGF beta 1
Thrombopoietin
(TPO)
Tie-1
Tie-2
TIMP-1
TIMP-2
TIMP-4
TNF alpha
TNF beta
(TNFSF1B)
TRAIL R3
(TNFRSF10C)
TRAIL R4
(TNFRSF10D)
uPAR
VE-Cadherin
(CDH5)
  • Gene Symbol: CDH5
  • Gene ID: 1003
  • Accession #: P33151
VEGF-A
VEGFR2
VEGFR3
VEGF-D

Species Detected

Human

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Cytokine Antibody Array C2000 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP??s ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Shang T., Yao B., Gao D., et al. A novel model of humanised keloid scarring in mice. Int Wound J. 2018 Feb;15(1):90-94. doi: 10.1111/iwj.12838.
    Species: Human
    Sample type: Conditioned Media
  2. Li MF., Zhang R., Li TT., et al. High Glucose Increases the Expression of Inflammatory Cytokine Genes in Macrophages Through H3K9 Methyltransferase Mechanism. J Interferon Cytokine Res. 2016 Jan;36(1):48-61. doi: 10.1089/jir.2014.0172
    Species: Human
    Sample type: Conditioned Media
  3. Jin H., Lee H., Heo J., et al. Senescence-Associated MCP-1 Secretion Is Dependent on a Decline in BMI1 in Human Mesenchymal Stromal Cells. Antioxid Redox Signal. 2016 Mar 20;24(9):471-85. doi: 10.1089/ars.2015.6359.
    Species: Human
    Sample type: Conditioned Media
  4. Jin HJ., Lee HJ., Heo J., et al. Senescence associated MCP-1 secretion is dependent on a decline in BMI1 in human mesenchymal stromal cells. Antioxidants & redox signalling [1523-0864], 2015, epub ahead of print.
    Species: Human
    Sample type: Conditioned Media
  5. Anderson J., et al. Phosphoproteomic Profiling Reveals IL6-mediated Paracrine Signaling within the Ewing Sarcoma Family of Tumors. Mol Cancer Res., Published Online First August 4, 2014; doi: 10.1158/1541-7786.MCR-14-0159
    Species: Human
    Sample type: Conditioned Media
  6. Shimizu, Masaki, Mondo Kuroda, and Michio Konishi. "Extensive Serum Biomarker Analysis in Patients with Enterohemorrhagic Escherichia Coli O111-induced Hemolytic-uremic Syndrome." Cytokine. ScienceDirect, Mar. 2014. Web.
    Species: Human
    Sample type: Serum
  7. Ho Ia., Toh H., Ng W., et al. Human bone marrow-derived mesenchymal stem cells suppress human glioma growth through inhibition of angiogenesis. Stem Cells. 2013 Jan;31(1):146-55. doi: 10.1002/stem.1247
    Species: Human
    Sample type: Conditioned Media
  8. Ho I., Toh H., Ng W., et al. Human Bone Marrow?Derived Mesenchymal Stem Cells Suppress Human Glioma Growth Through Inhibition of Angiogenesis, Stem Cells. 2013 Jan;31(1):146-55. doi: 10.1002/stem.1247.
    Species: Human
    Sample type: Conditioned Media
  9. Low-Marchelli JM, Ardi VC, Vizcarra EA, et al. Twist1 Induces CCL2 and Recruits Macrophages to Promote Angiogenesis. Cancer Res. 2013;73:662-671.
    Species: Human
    Sample type: Conditioned Media
  10. Wang T, Wang YY, Zhou R, et al. Bioactive proteins in healthy pregnancies and preeclampsia: relevance to hypertension and proteinuria. Chin Med J. 2013;126(11):2015-2020.
    Species: Human
    Sample type: Amniotic Fluid

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Analysis Tool Bundle Discount

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Total list price $1,548.33
Price for all $1,384.31

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.