Human Cytokine Array C2000

RayBio® C-Series Human Cytokine Antibody Array 2000 Kit. A combination of Human Cytokine Antibody Array C6, C7, & C8. Detects 174 Human Cytokines. Suitable for all liquid sample types.

AAH-CYT-2000-2
+

Antigen Information

Gene Symbols:
ADIPOQ | AGRP | ALCAM | ANG | ANGPT2 | AREG | AXL | BDNF | BMP4 | BMP5   View more

Assay Format

Number of Targets Detected:
120
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Adiponectin
(ACRP30)
Activin A
AgRP
ALCAM
(CD166)
Amphiregulin
Angiogenin
Angiopoietin-2
Axl
CD80
(B7-1)
BDNF
bFGF
BLC
(CXCL13)
BMP-4
BMP-5
BMP-6
BMP-7
beta-NGF
Betacellulin
(BTC)
Cardiotrophin-1
(CT-1)
CCL28
(MEC)
CD14
Ck beta 8-1
(CCL23)
CNTF
CTACK
(CCL27)
CXCL16
DR6
(TNFRSF21)
Dtk
EGF
EGFR
ENA-78
(CXCL5)
Endoglin
(CD105)
Eotaxin-1
(CCL11)
Eotaxin-2
(MPIF-2/CCL24)
Eotaxin-3
(CCL26)
ErbB3
E-Selectin
Fas
(TNFRSF6/Apo-1)
Fas Ligand
(TNFSF6)
FGF-4
FGF-6
FGF-7
(KGF)
FGF-9
Flt-3 Ligand
Fractalkine
(CX3CL1)
GCP-2
(CXCL6)
GCSF
GDNF
GITR
(TNFRSF18)
GITR Ligand
(TNFSF18)
GM-CSF
GRO alpha/beta/gamma
GRO alpha
(CXCL1)
HCC-4
(CCL16)
HGF
I-309
(TCA-3/CCL1)
ICAM-1
(CD54)
ICAM-2
(CD102)
ICAM-3
(CD50)
IFN-gamma
IGFBP-1
IGFBP-2
IGFBP-3
IGFBP-4
IGFBP-6
IGF-1
IGF-1 R
IGF-2
  • Gene Symbol: Igf2
  • Gene ID: 3481
  • Accession #: P01344
IL-1 R2
IL-1 R4
(ST2)
IL-1 R1
IL-10
IL-10 R beta
IL-11
IL-12 p40
IL-12 p70
IL-13
IL-13 R alpha 2
IL-15
IL-16
IL-17A
  • Gene Symbol: IL17A
  • Gene ID: 3605
  • Accession #: Q16552
IL-18 BP alpha
IL-18 R beta
(AcPL)
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-1 ra
(IL-1 F3)
IL-2
IL-2 R beta
(CD122)
IL-2 R gamma
(Common gamma Chain)
  • Gene Symbol: IL2RG
  • Gene ID: 3561
  • Accession #: P31785
IL-2 R alpha
IL-21 R
IL-3
IL-4
IL-5
IL-5 R alpha
IL-6
IL-6 R
IL-7
IL-8
(CXCL8)
IL-9
IP-10
(CXCL10)
I-TAC
(CXCL11)
LAP/TGF beta 1
  • Gene Symbol: TGFB1
  • Gene ID: 7040
  • Accession #: P01137
Leptin
Leptin R
  • Gene Symbol: LEPR
  • Gene ID: 3953
  • Accession #: P48357
LIF
Light
(TNFSF14)
L-Selectin
(CD62L)
Lymphotactin
(XCL1)
MCP-1
(CCL2)
MCP-2
(CCL8)
MCP-3
(MARC/CCL7)
MCP-4
(CCL13)
M-CSF
M-CSF R
MDC
(CCL22)
MIF
MIG
(CXCL9)
MIP-1 alpha
(CCL3)
MIP-1 beta
(CCL4)
MIP-1 delta
(CCL15)
MIP-3 alpha
(CCL20)
MIP-3 beta
(CCL19)
MMP-1
MMP-13
MMP-3
MMP-9
MPIF-1
(CCL23)
MSP alpha/beta
NAP-2
(PPBP/CXCL7)
NGFR
(TNFRSF16)
NT-3
NT-4
Oncostatin M
Osteoprotegerin
(TNFRSF11B)
PARC
(CCL18)
PDGF-AA
PDGF R alpha
PDGF R beta
PDGF-AB
PDGF-BB
PECAM-1
(CD31)
PLGF
Prolactin
  • Gene Symbol: PRL
  • Gene ID: 5617
  • Accession #: P01236
RANTES
(CCL5)
SCF
SCF R
(CD117/c-kit)
SDF-1 alpha
(CXCL12 alpha)
SDF-1 beta
(CXCL12 beta)
gp130
Siglec-5
(CD170)
TNF RII
(TNFRSF1B)
TNF RI
(TNFRSF1A)
TARC
(CCL17)
TECK
(CCL25)
TGF beta 2
TGF alpha
TGF beta 3
  • Gene Symbol: TGFB3
  • Gene ID: 7043
  • Accession #: P10600
TGF beta 1
Thrombopoietin
(TPO)
Tie-1
Tie-2
TIMP-1
TIMP-2
TIMP-4
TNF alpha
TNF beta
(TNFSF1B)
TRAIL R3
(TNFRSF10C)
TRAIL R4
(TNFRSF10D)
uPAR
VE-Cadherin
(CDH5)
  • Gene Symbol: CDH5
  • Gene ID: 1003
  • Accession #: P33151
VEGF-A
VEGFR2
VEGFR3
VEGF-D

Species Detected

Human

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Cytokine Antibody Array C2000 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP??s ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Huang, Shuai, et al. "Hypoxia of PC-3 prostate cancer cells enhances migration and vasculogenesis in vitro of bone marrow-derived endothelial progenitor cells by secretion of cytokines." Oncology reports 29.6 (2013): 2369-2377.
    Species: Human
    Sample type: Conditioned Media
  2. Wang, Wei Eric, et al. "Prolyl Hydroxylase Domain Protein 2 Silencing Enhances the Survival and Paracrine Function of Transplanted Adipose-Derived Stem Cells in Infarcted MyocardiumNovelty and Significance." Circulation research113.3 (2013): 288-300.
    Species: Human
    Sample type: Conditioned Media
  3. Benabbou, Nadia, et al. "Hospicells promote upregulation of the ATP-binding cassette genes by insulin-like growth factor-I via the JAK2/STAT3 signaling pathway in an ovarian cancer cell line." International journal of oncology 43.3 (2013): 685-694.
    Species: Human
    Sample type: Conditioned Media
  4. Azzazene, Dalel, et al. "Plasma endothelial protein C receptor influences innate immune response in ovarian cancer by decreasing the population of natural killer and TH17 helper cells." International journal of oncology 43.4 (2013): 1011-1018.
    Species: Human
    Sample type: Plasma
  5. Sung, Shian-Ying, et al. "Loss of let-7 microRNA upregulates IL-6 in bone marrow-derived mesenchymal stem cells triggering a reactive stromal response to prostate cancer." PloS one 8.8 (2013): e71637.
    Species: Human
    Sample type: Conditioned Media
  6. Chan KYY, Leung FWL, Lam HS, et al. Immunoregulatory Protein Profiles of Necrotizing Enterocolitis versus Spontaneous Intestinal Perforation in Preterm Infants. Neu J, ed. PLoS ONE. 2012;7(5):e36977. doi:10.1371/journal.pone.0036977.
    Species: Human
    Sample type: Plasma
  7. Ho L, Zhao W, Dams-O’Connor K, et al. Elevated Plasma MCP-1 Concentration Following Traumatic Brain Injury as a Potential “Predisposition” Factor Associated with an Increased Risk for Subsequent Development of Alzheimer’s Disease. Journal of Alzheimer’s disease?: JAD. 2012;31(2):301-313. doi:10.3233/JAD-2012-120598.
    Species: Human
    Sample type: Plasma
  8. Hsiao WC., Sung SY., Lioa CH., et al. Vitamin D3-inducible mesenchymal stem cell-based delivery of conditionally replicating adenoviruses effectively targets renal cell carcinoma and inhibits tumor growth. Mol Pharm. 2012 May 7;9(5):1396-408. doi: 10.1021/mp200649g.
    Species: Human
    Sample type: Conditioned Media
  9. Choi Y., Lim D., Lim S., Kim D. Effects of mixed feeder cells on the expansion of CD34? cells. J Biosci Bioeng. 2012 Mar;113(3):389-94. doi: 10.1016/j.jbiosc.2011.11.006.
    Species: Human
    Sample type: Conditioned Media
  10. Wang T., et al. Proteins in leaked amniotic fluid as biomarkers diagnostic for prelabor rupture of membranes. Proteomics Clin Appl 2011 Aug;5(7-8):415-21. doi: 10.1002/prca.201000123.
    Species: Human
    Sample type: Cervical-Vaginal Fluid

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.