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Home/Search results/Products/Arrays/Antibody Arrays/C-Series: Membrane-Based Arrays/Cytokine Arrays/Human Cytokine Array C2000

Manual / SDS

Description
Citations
Reviews
Files

Antigen Information

Gene Symbols:

Assay Format

Number of Targets Detected:

174

Species Detected:

Human

Compatible Sample Types:

Cell Culture Supernatants
Cell Lysates
Plasma
Serum
Tissue Lysates

Design Principle:

Sandwich-based

Method of Detection:

Chemiluminescence

Quantitative/Semi-Quantitative:

Semi-Quantitative

Solid Support:

Membrane

Product Specifications

Size:

2, 4, or 8 Sample Kit

Product Features

Easy to use
No specialized equipment needed
Compatible with nearly any liquid sample
Proven technology (many publications)
Highly sensitive (pg/ml)
Sandwich ELISA specificity
Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Adiponectin (ACRP30) Gene Symbol: ADIPOQ Gene ID: 9370 Accession #: Q15848 ELISA Kit: RayBio Human Acrp30 ELISA	Activin A Gene Symbol: INHBA Gene ID: 3624 Accession #: P08476 ELISA Kit: RayBio Human Activin A ELISA	AgRP Gene Symbol: AgRP Gene ID: 181 Accession #: O00253 ELISA Kit: RayBio Human AgRP ELISA	ALCAM (CD166) Gene Symbol: ALCAM Gene ID: 214 Accession #: Q13740 ELISA Kit: RayBio Human ALCAM ELISA	Amphiregulin Gene Symbol: AREG Gene ID: 374 Accession #: P15514 ELISA Kit: RayBio Human AREG ELISA
Angiogenin Gene Symbol: ANG Gene ID: 283 Accession #: P03950 ELISA Kit: RayBio Human Angiogenin ELISA	Angiopoietin-2 Gene Symbol: ANGPT2 Gene ID: 285 Accession #: O15123 ELISA Kit: RayBio Human ANGPT2 ELISA	Axl Gene Symbol: Axl Gene ID: 558 Accession #: P30530 ELISA Kit: RayBio Human Axl ELISA	CD80 (B7-1) Gene Symbol: CD80 Gene ID: 941 Accession #: P33681 ELISA Kit: RayBio Human CD80 ELISA	BDNF Gene Symbol: BDNF Gene ID: 627 Accession #: P23560 ELISA Kit: RayBio Human BDNF ELISA
bFGF Gene Symbol: FGF2 Gene ID: 2247 Accession #: P09038 ELISA Kit: RayBio Human bFGF ELISA	BLC (CXCL13) Gene Symbol: CXCL13 Gene ID: 10563 Accession #: O43927 ELISA Kit: RayBio Human BLC ELISA	BMP-4 Gene Symbol: BMP4 Gene ID: 652 Accession #: P12644 ELISA Kit: RayBio Human BMP-4 ELISA	BMP-5 Gene Symbol: BMP5 Gene ID: 653 Accession #: P22003 ELISA Kit: RayBio Human BMP-5 ELISA	BMP-6 Gene Symbol: BMP6 Gene ID: 654 Accession #: P22004 ELISA Kit: RayBio Human BMP-6 ELISA
BMP-7 Gene Symbol: BMP7 Gene ID: 655 Accession #: P18075 ELISA Kit: RayBio Human BMP-7 ELISA	beta-NGF Gene Symbol: NGF Gene ID: 4803 Accession #: P01138 ELISA Kit: RayBio Human beta-NGF ELISA	Betacellulin (BTC) Gene Symbol: BTC Gene ID: 685 Accession #: P35070 ELISA Kit: RayBio Human BTC ELISA	Cardiotrophin-1 (CT-1) Gene Symbol: CTF1 Gene ID: 1489 Accession #: Q16619 ELISA Kit: RayBio Human CT-1 ELISA	CCL28 (MEC) Gene Symbol: CCL28 Gene ID: 56477 Accession #: Q9NRJ3 ELISA Kit: RayBio Human CCL28 ELISA
CD14 Gene Symbol: CD14 Gene ID: 929 Accession #: P08571 ELISA Kit: RayBio Human CD14 ELISA	Ck beta 8-1 (CCL23) Gene Symbol: CCL23 Gene ID: 6368 Accession #: P55773 ELISA Kit: RayBio Human Ckbeta8 ELISA	CNTF Gene Symbol: CNTF Gene ID: 1270 Accession #: P26441 ELISA Kit: RayBio Human CNTF ELISA	CTACK (CCL27) Gene Symbol: CCL27 Gene ID: 10850 Accession #: Q9Y4X3 ELISA Kit: RayBio Human CTACK ELISA	CXCL16 Gene Symbol: CXCL16 Gene ID: 58191 Accession #: Q9H2A7 ELISA Kit: RayBio Human CXCL16 ELISA
DR6 (TNFRSF21) Gene Symbol: TNFRSF21 Gene ID: 27242 Accession #: O75509 ELISA Kit: RayBio Human DR6 ELISA	Dtk Gene Symbol: TYRO3 Gene ID: 7301 Accession #: Q06418 ELISA Kit: RayBio Human Dtk ELISA	EGF Gene Symbol: EGF Gene ID: 1950 Accession #: P01133 ELISA Kit: RayBio Human EGF ELISA	EGFR Gene Symbol: EGFR Gene ID: 1956 Accession #: P00533 ELISA Kit: RayBio Human EGFR ELISA	ENA-78 (CXCL5) Gene Symbol: CXCL5 Gene ID: 6374 Accession #: P42830 ELISA Kit: RayBio Human ENA-78 ELISA
Endoglin (CD105) Gene Symbol: ENG Gene ID: 2022 Accession #: P17813 ELISA Kit: RayBio Human Endoglin ELISA	Eotaxin-1 (CCL11) Gene Symbol: CCL11 Gene ID: 6356 Accession #: P51671 ELISA Kit: RayBio Human Eotaxin-1 ELISA	Eotaxin-2 (MPIF-2/CCL24) Gene Symbol: Ccl24 Gene ID: 6369 Accession #: O00175 ELISA Kit: RayBio Human Eotaxin-2 ELISA	Eotaxin-3 (CCL26) Gene Symbol: CCL26 Gene ID: 10344 Accession #: Q9Y258 ELISA Kit: RayBio Human Eotaxin-3 ELISA	ErbB3 Gene Symbol: ErbB3 Gene ID: 2065 Accession #: P21860 ELISA Kit: RayBio Human ErbB3 ELISA
E-Selectin Gene Symbol: SELE Gene ID: 6401 Accession #: P16581 ELISA Kit: RayBio Human E-Selectin ELISA	Fas (TNFRSF6/Apo-1) Gene Symbol: Fas Gene ID: 355 Accession #: P25445 ELISA Kit: RayBio Human Fas ELISA	Fas Ligand (TNFSF6) Gene Symbol: FASLG Gene ID: 356 Accession #: P48023 ELISA Kit: RayBio Human FasL ELISA	FGF-4 Gene Symbol: FGF4 Gene ID: 2249 Accession #: P08620 ELISA Kit: RayBio Human FGF-4 ELISA	FGF-6 Gene Symbol: FGF6 Gene ID: 2251 Accession #: P10767 ELISA Kit: RayBio Human FGF-6 ELISA
FGF-7 (KGF) Gene Symbol: FGF7 Gene ID: 2252 Accession #: P21781 ELISA Kit: RayBio Human FGF-7 ELISA	FGF-9 Gene Symbol: FGF9 Gene ID: 2254 Accession #: P31371 ELISA Kit: RayBio Human FGF-9 ELISA	Flt-3 Ligand Gene Symbol: FLT3LG Gene ID: 2323 Accession #: P49771 ELISA Kit: RayBio Human Flt-3 L ELISA	Fractalkine (CX3CL1) Gene Symbol: CX3CL1 Gene ID: 6376 Accession #: P78423 ELISA Kit: RayBio Human Fractalkine ELISA	GCP-2 (CXCL6) Gene Symbol: CXCL6 Gene ID: 6372 Accession #: P80162 ELISA Kit: RayBio Human GCP-2 ELISA
GCSF Gene Symbol: CSF3 Gene ID: 1440 Accession #: P09919 ELISA Kit: RayBio Human G-CSF ELISA	GDNF Gene Symbol: GDNF Gene ID: 2668 Accession #: P39905 ELISA Kit: RayBio Human GDNF ELISA	GITR (TNFRSF18) Gene Symbol: TNFRSF18 Gene ID: 8784 Accession #: Q9Y5U5 ELISA Kit: RayBio Human GITR ELISA	GITR Ligand (TNFSF18) Gene Symbol: TNFSF18 Gene ID: 8995 Accession #: Q9UNG2 ELISA Kit: RayBio Human GITR L ELISA	GM-CSF Gene Symbol: CSF2 Gene ID: 1437 Accession #: P04141 ELISA Kit: RayBio Human GM-CSF ELISA
GRO alpha/beta/gamma Gene Symbol: CXCL1/CXCL2/CXCL3 Gene ID: 2919/2920/2921 Accession #: P09341/P19875/P19876	GRO alpha (CXCL1) Gene Symbol: CXCL1 Gene ID: 2919 Accession #: P09341 ELISA Kit: RayBio Human GRO-alpha ELISA	HCC-4 (CCL16) Gene Symbol: CCL16 Gene ID: 6360 Accession #: O15467 ELISA Kit: RayBio Human HCC-4 ELISA	HGF Gene Symbol: HGF Gene ID: 3082 Accession #: P14210 ELISA Kit: RayBio Human HGF ELISA	I-309 (TCA-3/CCL1) Gene Symbol: CCL1 Gene ID: 6346 Accession #: P22362 ELISA Kit: RayBio Human I-309 ELISA
ICAM-1 (CD54) Gene Symbol: ICAM1 Gene ID: 3383 Accession #: P05362 ELISA Kit: RayBio Human sICAM-1 ELISA	ICAM-2 (CD102) Gene Symbol: ICAM2 Gene ID: 3384 Accession #: P13598 ELISA Kit: RayBio Human ICAM-2 ELISA	ICAM-3 (CD50) Gene Symbol: ICAM3 Gene ID: 3385 Accession #: P32942 ELISA Kit: RayBio Human ICAM-3 ELISA	IFN-gamma Gene Symbol: IFNG Gene ID: 3458 Accession #: P01579 ELISA Kit: RayBio Human IFN-gamma ELISA	IGFBP-1 Gene Symbol: IGFBP1 Gene ID: 3484 Accession #: P08833 ELISA Kit: RayBio Human IGFBP-1 ELISA
IGFBP-2 Gene Symbol: IGFBP2 Gene ID: 3485 Accession #: P18065 ELISA Kit: RayBio Human IGFBP-2 ELISA	IGFBP-3 Gene Symbol: IGFBP3 Gene ID: 3486 Accession #: P17936 ELISA Kit: RayBio Human IGFBP-3 ELISA	IGFBP-4 Gene Symbol: IGFBP4 Gene ID: 3487 Accession #: P22692 ELISA Kit: RayBio Human IGFBP-4 ELISA	IGFBP-6 Gene Symbol: IGFBP6 Gene ID: 3489 Accession #: P24592 ELISA Kit: RayBio Human IGFBP-6 ELISA	IGF-1 Gene Symbol: IGF1 Gene ID: 3479 Accession #: P05019 ELISA Kit: RayBio Human IGF-1 ELISA
IGF-1 R Gene Symbol: IGF1R Gene ID: 3480 Accession #: P08069 ELISA Kit: RayBio Human IGF-1 R ELISA	IGF-2 Gene Symbol: Igf2 Gene ID: 3481 Accession #: P01344	IL-1 R2 Gene Symbol: IL1R2 Gene ID: 7850 Accession #: P27930 ELISA Kit: RayBio Human IL-1 sRII ELISA	IL-1 R4 (ST2) Gene Symbol: IL1RL1 Gene ID: 9173 Accession #: Q01638 ELISA Kit: RayBio Human IL-1 R4 ELISA	IL-1 R1 Gene Symbol: IL1R1 Gene ID: 3554 Accession #: P14778 ELISA Kit: RayBio Human IL-1 sRI ELISA
IL-10 Gene Symbol: IL10 Gene ID: 3586 Accession #: P22301 ELISA Kit: RayBio Human IL-10 ELISA	IL-10 R beta Gene Symbol: IL10RB Gene ID: 3588 Accession #: Q08334 ELISA Kit: RayBio Human IL-10 R beta ELISA	IL-11 Gene Symbol: IL11 Gene ID: 3589 Accession #: P20809 ELISA Kit: RayBio Human IL-11 ELISA	IL-12 p40 Gene Symbol: IL12B Gene ID: 3593 Accession #: P29460 ELISA Kit: RayBio Human IL-12 p40 ELISA	IL-12 p70 Gene Symbol: IL12A Gene ID: 3592 Accession #: P29459 ELISA Kit: RayBio Human IL-12 p70 ELISA
IL-13 Gene Symbol: IL13 Gene ID: 3596 Accession #: P35225 ELISA Kit: RayBio Human IL-13 ELISA	IL-13 R alpha 2 Gene Symbol: IL13RA2 Gene ID: 3598 Accession #: Q14627 ELISA Kit: RayBio Human IL-13 Ra2 ELISA	IL-15 Gene Symbol: IL15 Gene ID: 3600 Accession #: P40933 ELISA Kit: RayBio Human IL-15 ELISA	IL-16 Gene Symbol: IL16 Gene ID: 3603 Accession #: Q14005 ELISA Kit: RayBio Human IL-16 ELISA	IL-17A Gene Symbol: IL17A Gene ID: 3605 Accession #: Q16552
IL-18 BP alpha Gene Symbol: IL18BP Gene ID: 10068 Accession #: O95998 ELISA Kit: RayBio Human IL-18 BPa ELISA	IL-18 R beta (AcPL) Gene Symbol: IL18RAP Gene ID: 8807 Accession #: O95256 ELISA Kit: RayBio Human IL-18 R beta ELISA	IL-1 alpha (IL-1 F1) Gene Symbol: IL1A Gene ID: 3552 Accession #: P01583 ELISA Kit: RayBio Human IL-1 alpha ELISA	IL-1 beta (IL-1 F2) Gene Symbol: IL1B Gene ID: 3553 Accession #: P01584 ELISA Kit: RayBio Human IL-1 beta ELISA	IL-1 ra (IL-1 F3) Gene Symbol: IL1RN Gene ID: 3557 Accession #: P18510 ELISA Kit: RayBio Human IL-1 RA ELISA
IL-2 Gene Symbol: IL2 Gene ID: 3558 Accession #: P60568 ELISA Kit: RayBio Human IL-2 ELISA	IL-2 R beta (CD122) Gene Symbol: IL2RB Gene ID: 3560 Accession #: P14784 ELISA Kit: RayBio Human IL-2 R beta ELISA	IL-2 R gamma (Common gamma Chain) Gene Symbol: IL2RG Gene ID: 3561 Accession #: P31785	IL-2 R alpha Gene Symbol: IL2RA Gene ID: 3559 Accession #: P01589 ELISA Kit: RayBio Human IL-2 R alpha ELISA	IL-21 R Gene Symbol: IL21R Gene ID: 50615 Accession #: Q9HBE5 ELISA Kit: RayBio Human IL-21 R ELISA
IL-3 Gene Symbol: IL3 Gene ID: 3562 Accession #: P08700 ELISA Kit: RayBio Human IL-3 ELISA	IL-4 Gene Symbol: IL4 Gene ID: 3565 Accession #: P05112 ELISA Kit: RayBio Human IL-4 ELISA	IL-5 Gene Symbol: IL5 Gene ID: 3567 Accession #: P05113 ELISA Kit: RayBio Human IL-5 ELISA	IL-5 R alpha Gene Symbol: IL5RA Gene ID: 3568 Accession #: Q01344 ELISA Kit: RayBio Human IL-5 R alpha ELISA	IL-6 Gene Symbol: IL6 Gene ID: 3569 Accession #: P05231 ELISA Kit: RayBio Human IL-6 ELISA
IL-6 R Gene Symbol: IL6R Gene ID: 3570 Accession #: P08887 ELISA Kit: RayBio Human IL-6 sR ELISA	IL-7 Gene Symbol: IL7 Gene ID: 3574 Accession #: P13232 ELISA Kit: RayBio Human IL-7 ELISA	IL-8 (CXCL8) Gene Symbol: IL8 Gene ID: 3576 Accession #: P10145 ELISA Kit: RayBio Human IL-8 ELISA	IL-9 Gene Symbol: IL9 Gene ID: 3578 Accession #: P15248 ELISA Kit: RayBio Human IL-9 ELISA	IP-10 (CXCL10) Gene Symbol: CXCL10 Gene ID: 3627 Accession #: P02778 ELISA Kit: RayBio Human IP-10 ELISA
I-TAC (CXCL11) Gene Symbol: CXCL11 Gene ID: 6373 Accession #: O14625 ELISA Kit: RayBio Human I-TAC ELISA	LAP/TGF beta 1 Gene Symbol: TGFB1 Gene ID: 7040 Accession #: P01137	Leptin Gene Symbol: LEP Gene ID: 3952 Accession #: P41159 ELISA Kit: RayBio Human Leptin ELISA	Leptin R Gene Symbol: LEPR Gene ID: 3953 Accession #: P48357	LIF Gene Symbol: LIF Gene ID: 3976 Accession #: P15018 ELISA Kit: RayBio Human LIF ELISA
Light (TNFSF14) Gene Symbol: TNFSF14 Gene ID: 8740 Accession #: O43557 ELISA Kit: RayBio Human LIGHT ELISA	L-Selectin (CD62L) Gene Symbol: SELL Gene ID: 6402 Accession #: P14151 ELISA Kit: RayBio Human L-Selectin ELISA	Lymphotactin (XCL1) Gene Symbol: XCL1 Gene ID: 6375 Accession #: P47992 ELISA Kit: RayBio Human Lympotactin ELISA	MCP-1 (CCL2) Gene Symbol: CCL2 Gene ID: 6347 Accession #: P13500 ELISA Kit: RayBio Human MCP-1 ELISA	MCP-2 (CCL8) Gene Symbol: CCL8 Gene ID: 6355 Accession #: P80075 ELISA Kit: RayBio Human MCP-2 ELISA
MCP-3 (MARC/CCL7) Gene Symbol: CCL7 Gene ID: 6354 Accession #: P80098 ELISA Kit: RayBio Human MCP-3 ELISA	MCP-4 (CCL13) Gene Symbol: CCL13 Gene ID: 6357 Accession #: Q99616 ELISA Kit: RayBio Human MCP-4 ELISA	M-CSF Gene Symbol: CSF1 Gene ID: 1435 Accession #: P09603 ELISA Kit: RayBio Human M-CSF ELISA	M-CSF R Gene Symbol: CSF1R Gene ID: 1436 Accession #: P07333 ELISA Kit: RayBio Human M-CSF R ELISA	MDC (CCL22) Gene Symbol: CCL22 Gene ID: 6367 Accession #: O00626 ELISA Kit: RayBio Human MDC ELISA
MIF Gene Symbol: MIF Gene ID: 4282 Accession #: P14174 ELISA Kit: RayBio Human MIF ELISA	MIG (CXCL9) Gene Symbol: CXCL9 Gene ID: 4283 Accession #: Q07325 ELISA Kit: RayBio Human MIG ELISA	MIP-1 alpha (CCL3) Gene Symbol: CCL3 Gene ID: 6348 Accession #: P10147 ELISA Kit: RayBio Human MIP-1 alpha ELISA	MIP-1 beta (CCL4) Gene Symbol: CCL4 Gene ID: 6351 Accession #: P13236 ELISA Kit: RayBio Human MIP-1 beta ELISA	MIP-1 delta (CCL15) Gene Symbol: CCL15 Gene ID: 6359 Accession #: Q16663 ELISA Kit: RayBio Human MIP-1 delta ELISA
MIP-3 alpha (CCL20) Gene Symbol: CCL20 Gene ID: 6364 Accession #: P78556 ELISA Kit: RayBio Human MIP-3 alpha ELISA	MIP-3 beta (CCL19) Gene Symbol: CCL19 Gene ID: 6363 Accession #: Q99731 ELISA Kit: RayBio Human MIP-3 beta ELISA	MMP-1 Gene Symbol: MMP1 Gene ID: 4312 Accession #: P03956 ELISA Kit: RayBio Human MMP-1 ELISA	MMP-13 Gene Symbol: MMP13 Gene ID: 4322 Accession #: P45452 ELISA Kit: RayBio Human MMP-13 ELISA	MMP-3 Gene Symbol: MMP3 Gene ID: 4314 Accession #: P08254 ELISA Kit: RayBio Human MMP-3 ELISA
MMP-9 Gene Symbol: MMP9 Gene ID: 4318 Accession #: P14780 ELISA Kit: RayBio Human MMP-9 ELISA	MPIF-1 (CCL23) Gene Symbol: CCL23 Gene ID: 6368 Accession #: P55773 ELISA Kit: RayBio Human MPIF-1 ELISA	MSP alpha/beta Gene Symbol: MST1 Gene ID: 4485 Accession #: P26927 ELISA Kit: RayBio Human MSP ELISA	NAP-2 (PPBP/CXCL7) Gene Symbol: Ppbp Gene ID: 5473 Accession #: P02775 ELISA Kit: RayBio Human NAP-2 ELISA	NGFR (TNFRSF16) Gene Symbol: NGFR Gene ID: 4804 Accession #: P08138 ELISA Kit: RayBio Human NGF R ELISA
NT-3 Gene Symbol: NTF3 Gene ID: 4908 Accession #: P20783 ELISA Kit: RayBio Human NT-3 ELISA	NT-4 Gene Symbol: NTF4 Gene ID: 4909 Accession #: P34130 ELISA Kit: RayBio Human NT-4 ELISA	Oncostatin M Gene Symbol: OSM Gene ID: 5008 Accession #: P13725 ELISA Kit: RayBio Human OSM ELISA	Osteoprotegerin (TNFRSF11B) Gene Symbol: TNFRSF11B Gene ID: 4982 Accession #: O00300 ELISA Kit: RayBio Human OPG ELISA	PARC (CCL18) Gene Symbol: CCL18 Gene ID: 6362 Accession #: P55774 ELISA Kit: RayBio Human PARC ELISA
PDGF-AA Gene Symbol: PDGFA Gene ID: 5154 Accession #: P04085 ELISA Kit: RayBio Human PDGF-AA ELISA	PDGF R alpha Gene Symbol: PDGFRA Gene ID: 5156 Accession #: P16234 ELISA Kit: RayBio Human PDGF R alpha ELISA	PDGF R beta Gene Symbol: PDGFRB Gene ID: 5159 Accession #: P09619 ELISA Kit: RayBio Human PDGF R beta ELISA	PDGF-AB Gene Symbol: PDGFB Gene ID: 5155 Accession #: P01127 ELISA Kit: RayBio Human PDGF-AB ELISA	PDGF-BB Gene Symbol: PDGFB Gene ID: 5155 Accession #: P01127 ELISA Kit: RayBio Human PDGF-BB ELISA
PECAM-1 (CD31) Gene Symbol: PECAM1 Gene ID: 5175 Accession #: P16284 ELISA Kit: RayBio Human PECAM-1 ELISA	PLGF Gene Symbol: PGF Gene ID: 5228 Accession #: P49763 ELISA Kit: RayBio Human PLGF ELISA	Prolactin Gene Symbol: PRL Gene ID: 5617 Accession #: P01236	RANTES (CCL5) Gene Symbol: CCL5 Gene ID: 6352 Accession #: P13501 ELISA Kit: RayBio Human RANTES ELISA	SCF Gene Symbol: KITLG Gene ID: 4254 Accession #: P21583 ELISA Kit: RayBio Human SCF ELISA
SCF R (CD117/c-kit) Gene Symbol: KIT Gene ID: 3815 Accession #: P10721 ELISA Kit: RayBio Human SCF R ELISA	SDF-1 alpha (CXCL12 alpha) Gene Symbol: CXCL12 Gene ID: 6387 Accession #: P48061 ELISA Kit: RayBio Human SDF-1 alpha ELISA	SDF-1 beta (CXCL12 beta) Gene Symbol: CXCL12 Gene ID: 6387 Accession #: P48061 ELISA Kit: RayBio Human SDF-1 beta ELISA	gp130 Gene Symbol: Il6st Gene ID: 3572 Accession #: P40189 ELISA Kit: RayBio Human sgp130 ELISA	Siglec-5 (CD170) Gene Symbol: SIGLEC5 Gene ID: 8778 Accession #: O15389 ELISA Kit: RayBio Human Siglec-5 ELISA
TNF RII (TNFRSF1B) Gene Symbol: TNFRSF1B Gene ID: 7133 Accession #: P20333 ELISA Kit: RayBio Human sTNFRII ELISA	TNF RI (TNFRSF1A) Gene Symbol: TNFRSF1A Gene ID: 7132 Accession #: P19438 ELISA Kit: RayBio Human sTNFRI ELISA	TARC (CCL17) Gene Symbol: CCL17 Gene ID: 6361 Accession #: Q92583 ELISA Kit: RayBio Human TARC ELISA	TECK (CCL25) Gene Symbol: CCL25 Gene ID: 6370 Accession #: O15444 ELISA Kit: RayBio Human TECK ELISA	TGF beta 2 Gene Symbol: TGFB2 Gene ID: 7042 Accession #: P61812 ELISA Kit: RayBio Human TGF-beta 2 ELISA
TGF alpha Gene Symbol: TGFA Gene ID: 7039 Accession #: P01135 ELISA Kit: RayBio Human TGF-alpha ELISA	TGF beta 3 Gene Symbol: TGFB3 Gene ID: 7043 Accession #: P10600	TGF beta 1 Gene Symbol: TGFB1 Gene ID: 7040 Accession #: P01137 ELISA Kit: RayBio Human TGF-beta 1 ELISA	Thrombopoietin (TPO) Gene Symbol: THPO Gene ID: 7066 Accession #: P40225 ELISA Kit: RayBio Human TPO ELISA	Tie-1 Gene Symbol: TIE1 Gene ID: 7075 Accession #: P35590 ELISA Kit: RayBio Human Tie-1 ELISA
Tie-2 Gene Symbol: TEK Gene ID: 7010 Accession #: Q02763 ELISA Kit: RayBio Human Tie-2 ELISA	TIMP-1 Gene Symbol: TIMP1 Gene ID: 7076 Accession #: P01033 ELISA Kit: RayBio Human TIMP-1 ELISA	TIMP-2 Gene Symbol: TIMP2 Gene ID: 7077 Accession #: P16035 ELISA Kit: RayBio Human TIMP-2 ELISA	TIMP-4 Gene Symbol: TIMP4 Gene ID: 7079 Accession #: Q99727 ELISA Kit: RayBio Human TIMP-4 ELISA	TNF alpha Gene Symbol: TNF Gene ID: 7124 Accession #: P01375 ELISA Kit: RayBio Human TNF-alpha ELISA
TNF beta (TNFSF1B) Gene Symbol: LTA Gene ID: 4049 Accession #: P01374 ELISA Kit: RayBio Human TNF-beta ELISA	TRAIL R3 (TNFRSF10C) Gene Symbol: TNFRSF10C Gene ID: 8794 Accession #: O14798 ELISA Kit: RayBio Human TRAIL R3 ELISA	TRAIL R4 (TNFRSF10D) Gene Symbol: TNFRSF10D Gene ID: 8793 Accession #: Q9UBN6 ELISA Kit: RayBio Human TRAIL R4 ELISA	uPAR Gene Symbol: PLAUR Gene ID: 5329 Accession #: Q03405 ELISA Kit: RayBio Human uPAR ELISA	VE-Cadherin (CDH5) Gene Symbol: CDH5 Gene ID: 1003 Accession #: P33151
VEGF-A Gene Symbol: VEGFA Gene ID: 7422 Accession #: P15692 ELISA Kit: RayBio Human VEGF-A ELISA	VEGFR2 Gene Symbol: KDR Gene ID: 3791 Accession #: P35968 ELISA Kit: RayBio Human VEGF R2 ELISA	VEGFR3 Gene Symbol: FLT4 Gene ID: 2324 Accession #: P35916 ELISA Kit: RayBio Human VEGF R3 ELISA	VEGF-D Gene Symbol: FIGF Gene ID: 2277 Accession #: O43915 ELISA Kit: RayBio Human VEGF-D ELISA

Species Detected

Human

Application Notes

Suggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process

Kit Components

Human Cytokine Antibody Array C2000 Membranes
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated HRP
Detection Buffer C
Detection Buffer D
Lysis Buffer
8-Well Incubation Tray
Plastic Sheets
Array Templates
Manual

Other Materials Required

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system (such as UVP??s ChemiDoc-It^® or EpiChem II Benchtop Darkroom or GE's ImageQuant^™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Protocol Outline

Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20^°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20^°C and all other reagents undiluted at 4^°C for no more than 3 months.

Belletti B, Vaidya J, Andrea S, Entschladen F, Roncadin M, et al. Targeted Intraoperative Radiotherapy Impairs the Stimulation of Breast Cancer Cell Proliferation and Invasion Caused by Surgical Wounding. Clin Cancer Res March 1, 2008 14; 1325
Species: Human
Sample type: Serum
Fujita K,Ewing C M ,Sokoll L J, Elliott D J, Cunningham M, et al.Cytokine Profiling of Prostatic Fluid From Cancerous Prostate Glands Identifies Cytokines Associated With Extent of Tumor and Inflammation. Prostate. 2008;68:872-882.
Species: Human
Sample type: Prostatic Fluid
Steed D, Trumpower C, Duffy D, Smith C, Marshall V, et al. Amnion-derived Cellular Cytokine Solution A Physiological Combination of Cytokines for Wound Healing. Eplasty. 2008;8:e18.
Species: Human
Sample type: Conditioned Media
Ukropec J, Penesova A, Kopkova M, Pura M, Vlcek M,et al.Adipokine Protein Expression Pattern in Growth Hormone Deficiency Predisposes to the Increased Fat Cell Size and the Whole Body Metabolic Derangements. J Clin Endocrinol Metab. 2008;93: 2255-2262
Species: Human
Sample type: Tissue Lysate
Yang CC, Shih YH, Ko MH, Hsu SY,Cheng H, et al. Transplantation of Human Umbilical Mesenchymal Stem Cells from Whartons Jelly after Complete Transection of the Rat Spinal Cord. PLoS ONE. 2008;3(10):e3336. doi:10.1371/journal.pone.0003336
Species: Human
Sample type: Tissue Lysate
Kim N, Dabrowska A, Jenner RG, Aldovini A. Human and Simian Immunodeficiency Virus-Mediated Upregulation of the Apoptotic Factor TRAIL Occurs in Antigen-Presenting Cells from AIDS-Susceptible but Not from AIDS-Resistant Species. J Virol. 2007; 81(14):7584-7597.
Species: Human
Sample type: Conditioned Media
Lu Y, Cai Z, Xiao G, Keller ET, et al. Monocyte Chemotactic Protein-1 Mediates Prostate Cancer-Induced Bone Resorption. Cancer Res. 2007; 67(8):3646-3653.
Species: Human
Sample type: Conditioned Media
Parekkadan B, van Poll D, Suganuma K, Carter EA, Berthiaume F, et al. Mesenchymal Stem Cell-Derived Molecules Reverse Fulminant Hepatic Failure. PLoS ONE.2007;2(9): e941. doi:10.1371/journal.pone.0000941
Species: Human
Sample type: Conditioned Media
Liu B-C, Zhang L, Lv L, Wang Y, Liu D, Zhang X. Application of Antibody Array Technology in the Analysis of Urinary Cytokine Profiles in Patients with Chronic Kidney Disease. Am J Nephrol. 2006;26(5):483-490.
Species: Human
Sample type: Urine

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Manual

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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