Human Cytokine Array C2000

RayBio® C-Series Human Cytokine Antibody Array 2000 Kit. A combination of Human Cytokine Antibody Array C6, C7, & C8. Detects 174 Human Cytokines. Suitable for all liquid sample types.

$1,314.00  
AAH-CYT-2000-2
+

Assay Format

Number of Targets Detected:
174
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Protein Information

Gene Symbols:
ADIPOQ | AGRP | ALCAM | ANG | ANGPT2 | AREG | AXL | BDNF | BMP4 | BMP5   View more

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Adiponectin
(ACRP30)
Activin A
AgRP
ALCAM
(CD166)
Amphiregulin
Angiogenin
Angiopoietin-2
Axl
CD80
(B7-1)
BDNF
bFGF
BLC
(CXCL13)
BMP-4
BMP-5
BMP-6
BMP-7
beta-NGF
Betacellulin
(BTC)
Cardiotrophin-1
(CT-1)
CCL28
(MEC)
CD14
Ck beta 8-1
(CCL23)
CNTF
CTACK
(CCL27)
CXCL16
DR6
(TNFRSF21)
Dtk
EGF
EGFR
ENA-78
(CXCL5)
Endoglin
(CD105)
Eotaxin-1
(CCL11)
Eotaxin-2
(MPIF-2/CCL24)
Eotaxin-3
(CCL26)
ErbB3
E-Selectin
Fas
(TNFRSF6/Apo-1)
Fas Ligand
(TNFSF6)
FGF-4
FGF-6
FGF-7
(KGF)
FGF-9
Flt-3 Ligand
Fractalkine
(CX3CL1)
GCP-2
(CXCL6)
GCSF
GDNF
GITR
(TNFRSF18)
GITR Ligand
(TNFSF18)
GM-CSF
GRO alpha/beta/gamma
GRO alpha
(CXCL1)
HCC-4
(CCL16)
HGF
I-309
(TCA-3/CCL1)
ICAM-1
(CD54)
ICAM-2
(CD102)
ICAM-3
(CD50)
IFN-gamma
IGFBP-1
IGFBP-2
IGFBP-3
IGFBP-4
IGFBP-6
IGF-1
IGF-1 R
IGF-2
  • Gene Symbol: Igf2
  • Gene ID: 3481
  • Accession #: P01344
IL-1 R2
IL-1 R4
(ST2)
IL-1 R1
IL-10
IL-10 R beta
IL-11
IL-12 p40
IL-12 p70
IL-13
IL-13 R alpha 2
IL-15
IL-16
IL-17A
  • Gene Symbol: IL17A
  • Gene ID: 3605
  • Accession #: Q16552
IL-18 BP alpha
IL-18 R beta
(AcPL)
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-1 ra
(IL-1 F3)
IL-2
IL-2 R beta
(CD122)
IL-2 R gamma
(Common gamma Chain)
  • Gene Symbol: IL2RG
  • Gene ID: 3561
  • Accession #: P31785
IL-2 R alpha
IL-21 R
IL-3
IL-4
IL-5
IL-5 R alpha
IL-6
IL-6 R
IL-7
IL-8
(CXCL8)
IL-9
IP-10
(CXCL10)
I-TAC
(CXCL11)
LAP/TGF beta 1
  • Gene Symbol: TGFB1
  • Gene ID: 7040
  • Accession #: P01137
Leptin
Leptin R
  • Gene Symbol: LEPR
  • Gene ID: 3953
  • Accession #: P48357
LIF
Light
(TNFSF14)
L-Selectin
(CD62L)
Lymphotactin
(XCL1)
MCP-1
(CCL2)
MCP-2
(CCL8)
MCP-3
(MARC/CCL7)
MCP-4
(CCL13)
M-CSF
M-CSF R
MDC
(CCL22)
MIF
MIG
(CXCL9)
MIP-1 alpha
(CCL3)
MIP-1 beta
(CCL4)
MIP-1 delta
(CCL15)
MIP-3 alpha
(CCL20)
MIP-3 beta
(CCL19)
MMP-1
MMP-13
MMP-3
MMP-9
MPIF-1
(CCL23)
MSP alpha/beta
NAP-2
(PPBP/CXCL7)
NGFR
(TNFRSF16)
NT-3
NT-4
Oncostatin M
Osteoprotegerin
(TNFRSF11B)
PARC
(CCL18)
PDGF-AA
PDGF R alpha
PDGF R beta
PDGF-AB
PDGF-BB
PECAM-1
(CD31)
PLGF
Prolactin
  • Gene Symbol: PRL
  • Gene ID: 5617
  • Accession #: P01236
RANTES
(CCL5)
SCF
SCF R
(CD117/c-kit)
SDF-1 alpha
(CXCL12 alpha)
SDF-1 beta
(CXCL12 beta)
gp130
Siglec-5
(CD170)
TNF RII
(TNFRSF1B)
TNF RI
(TNFRSF1A)
TARC
(CCL17)
TECK
(CCL25)
TGF beta 2
TGF alpha
TGF beta 3
  • Gene Symbol: TGFB3
  • Gene ID: 7043
  • Accession #: P10600
TGF beta 1
Thrombopoietin
(TPO)
Tie-1
Tie-2
TIMP-1
TIMP-2
TIMP-4
TNF alpha
TNF beta
(TNFSF1B)
TRAIL R3
(TNFRSF10C)
TRAIL R4
(TNFRSF10D)
uPAR
VE-Cadherin
(CDH5)
  • Gene Symbol: CDH5
  • Gene ID: 1003
  • Accession #: P33151
VEGF-A
VEGFR2
VEGFR3
VEGF-D
 

Species Detected

Human

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Cytokine Antibody Array C2000 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP??s ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Shih, Daniel Tzu?Bi, et al. "Expansion of adipose tissue mesenchymal stromal progenitors in serum?free medium supplemented with virally inactivated allogeneic human platelet lysate." Transfusion 51.4 (2011): 770-778.
    Species: Human
    Sample type: Conditioned Media (S/D-PL and FBS MSCs)
  2. Parekkadan B, van Poll D, Suganuma K, Carter EA, Berthiaume F, et al. Mesenchymal Stem Cell-Derived Molecules Reverse Fulminant Hepatic Failure. PLoS ONE.2007;2(9): e941. doi:10.1371/journal.pone.0000941
    Species: Human
    Sample type: Conditioned Media (serum-free medium)
  3. Yang CC, Shih YH, Ko MH, Hsu SY,Cheng H, et al. Transplantation of Human Umbilical Mesenchymal Stem Cells from Wharton’s Jelly after Complete Transection of the Rat Spinal Cord. PLoS ONE. 2008;3(10):e3336. doi:10.1371/journal.pone.0003336
    Species: Human
    Sample type: Tissue Lysate ( spinal cord- tissue Lysate )
  4. Chan KYY, Leung FWL, Lam HS, et al. Immunoregulatory Protein Profiles of Necrotizing Enterocolitis versus Spontaneous Intestinal Perforation in Preterm Infants. Neu J, ed. PLoS ONE. 2012;7(5):e36977. doi:10.1371/journal.pone.0036977.
    Species: Human
    Sample type: Plasma (Necrotizing enterocolitis and Spontaneousl intestinal performation patients)
  5. Walenda, Thomas, et al. "Serum after autologous transplantation stimulates proliferation and expansion of human hematopoietic progenitor cells." PLoS One 6.3 (2011): e18012.
    Species: Human
    Sample type: Serum
  6. Rudnick, Jenny A., et al. "Functional heterogeneity of breast fibroblasts is defined by a prostaglandin secretory phenotype that promotes expansion of cancer-stem like cells." PloS one 6.9 (2011): e24605.
    Species: Human
    Sample type: Conditioned Media (primary fibroblasts from human breast tumors and reduction mammoplasty tissues)
  7. Moorefield, Emily C., et al. "Cloned, CD117 selected human amniotic fluid stem cells are capable of modulating the immune response." PloS one 6.10 (2011): e26535.
    Species: Human
    Sample type: Conditioned Media (AFS cells or BM-MSC s)
  8. Sung, Shian-Ying, et al. "Loss of let-7 microRNA upregulates IL-6 in bone marrow-derived mesenchymal stem cells triggering a reactive stromal response to prostate cancer." PloS one 8.8 (2013): e71637.
    Species: Human
    Sample type: Conditioned Media (3A6RWV and cancer-associated 3A6LNCaP, 3A6C4-2 and 3A6PC3 cells)
  9. Huang, Shuai, et al. "Hypoxia of PC-3 prostate cancer cells enhances migration and vasculogenesis in vitro of bone marrow-derived endothelial progenitor cells by secretion of cytokines." Oncology reports 29.6 (2013): 2369-2377.
    Species: Human
    Sample type: Conditioned Media (normoxic or hypoxic PC-3 cells )
  10. Choi Y., Lim D., Lim S., Kim D. Effects of mixed feeder cells on the expansion of CD34? cells. J Biosci Bioeng. 2012 Mar;113(3):389-94. doi: 10.1016/j.jbiosc.2011.11.006.
    Species: Human
    Sample type: Conditioned Media (CD34+ feeder cells and mixed populations of MSCs)

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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