Human EGFR Phosphorylation Array C1

$300.00
AAH-PER-1-2

RayBio® C-Series Human EGFR Phosphorylation Antibody Array 1 Kit. Detects 17 site specific and pan Human EGFRs. Suitable for all liquid sample types but intended for use with cell and tissue lysates.

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Antigen Information

Gene Symbol:
  • EGFR
  • ERBB2
  • ERBB3
  • ERBB4

Assay Format

Pathway:
  • HER/ErbB Signaling
Number of Targets Detected:
17
Species Detected:
  • Human
Compatible Sample Types:
  • Serum
  • Plasma
  • Cell Culture Supernatants
  • Cell Lysates
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
EGFR
(P-Tyr845)
  • Gene Symbol: EGFR
  • Gene ID: 1956
  • Accession #: P00533
EGFR
(P-Tyr992)
EGFR
(P-Tyr1045)
EGFR
(P-Tyr1068)
EGFR
(P-Tyr1086)
EGFR
(P-Tyr1148)
  • Gene Symbol: EGFR
  • Gene ID: 1956
  • Accession #: P00533
EGFR
(P-Tyr1173)
  • Gene Symbol: EGFR
  • Gene ID: 1956
  • Accession #: P00533
EGFR
(P-Ser1046/1047)
  • Gene Symbol: EGFR
  • Gene ID: 1956
  • Accession #: P00533
EGFR
(P-Ser1070)
ErbB2
(P-Tyr877)
  • Gene Symbol: ErbB2
  • Gene ID: 2064
  • Accession #: P04626
ErbB2
(P-Tyr1112)
  • Gene Symbol: ErbB2
  • Gene ID: 2064
  • Accession #: P04626
ErbB2
(P-Tyr 1221/1222)
  • Gene Symbol: ErbB2
  • Gene ID: 2064
  • Accession #: P04626
ErbB2
(P-Tyr1248)
  • Gene Symbol: ErbB2
  • Gene ID: 2064
  • Accession #: P04626
ErbB2
(P-Thr686)
  • Gene Symbol: ErbB2
  • Gene ID: 2064
  • Accession #: P04626
ErbB2
(P-Ser1113)
  • Gene Symbol: ErbB2
  • Gene ID: 2064
  • Accession #: P04626
ErbB3
(P-Tyr1289)
  • Gene Symbol: ErbB3
  • Gene ID: 2065
  • Accession #: P21860
ErbB4
(P-Tyr1284)
  • Gene Symbol: ErbB4
  • Gene ID: 2066
  • Accession #: Q15303

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human EGFR Phosphorylation Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • Protease Inhibitor Cocktail
  • Phosphatase Inhibitor Cocktail Set I
  • Phosphatase Inhibitor Cocktail II
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Kato K., Iwama H., Yamashita T., et al. The anti-diabetic drug metformin inhibits pancreatic cancer cell proliferation in vitro and in vivo: Study of the microRNAs associated with the antitumor effect of metformin. Oncol Rep. 2016 Mar;35(3):1582-92. doi: 10.3892/or.2015.4496
    Species: Human
    Sample type: Tissue Lysate
  2. Rios-Doria J., Sabol D., Chesebrough J., et al. A Monoclonal Antibody to ADAM17 Inhibits Tumor Growth by Inhibiting EGFR and Non-EGFR-Mediated Pathways. Mol Cancer Ther. 2015 Jul;14(7):1637-49. doi: 10.1158/1535-7163.MCT-14-1040.
    Species: Human
    Sample type: Tissue Lysate
  3. Montermini L., Meehan B., Farnier D., et al. Inhibition of oncogenic epidermal growth factor receptor kinase triggers release of exosome-like extracellular vesicles and impacts their phosphoprotein and DNA content. J Biol Chem. 2015 Oct 2;290(40):24534-46. doi: 10.1074/jbc.M115.679217.
    Species: Human
    Sample type: Extracellular Vesicles
  4. Arnold C., et al. Rac1 as a multifunctional therapeutic target to prevent and combat cancer metastasis. Oncoscience 2014, Vol.1, No.8
    Species: Human
    Sample type: Cell Lysate
  5. Panupinthu N., et al. Self-reinforcing loop of amphiregulin and Y-box binding protein-1 contributes to poor outcomes in ovarian cancer. Oncogene (2014) 33, 2846-2856; doi:10.1038/onc.2013.259
    Species: Human
    Sample type: Cell Lysate
  6. Slanina H., et al. Role of Epidermal Growth Factor Receptor Signaling in the Interaction of Neisseria meningitidis with Endothelial Cells. Infect. Immun. March 2014 vol. 82 no. 3 1243-1255
    Species: Human
    Sample type: Cell Lysate
  7. Cheng KW, Wong CC, Alston N, et al. Aerosol administration of phospho-sulindac inhibits lung tumorigenesis. Mol. Cancer Ther. 2013 May 3; 12: 1417-1428
    Species: Human
    Sample type: Cell Lysate
  8. Siu IM., et al. Erlotinib Inhibits Growth of a Patient-Derived Chordoma Xenograft . Published: November 15, 2013 DOI: 10.1371/journal.pone.0078895
    Species: Human
    Sample type: Cell Lysate
  9. Kesanakurti D., et al. Functional cooperativity by direct interaction between PAK4 and MMP-2 in the regulation of anoikis resistance, migration and invasion in glioma. Cell Death and Disease (2012) 3, e445; doi:10.1038/cddis.2012.182
    Species: Human
    Sample type: Cell Lysate
  10. Kesanakurti D., Chetty C., Rajasekhar Maddirela D., et al. Functional cooperativity by direct interaction between PAK4 and MMP-2 in the regulation of anoikis resistance, migration and invasion in glioma. Cell Death Dis. 2012 Dec 20;3:e445. doi: 10.1038/cddis.2012.182.
    Species: Human
    Sample type: Cell Lysate
I used this array to test the cell lysates from epidermoid carcinoma cell line exposed to EGF, it works well.
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Total list price $415.00
Price for all $334.50

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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