Human EGFR Phosphorylation Array C1

$300.00
AAH-PER-1-2

RayBio® C-Series Human EGFR Phosphorylation Antibody Array 1 Kit. Detects 17 site specific and pan Human EGFRs. Suitable for all liquid sample types but intended for use with cell and tissue lysates.

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Antigen Information

Gene Symbol:
  • EGFR
  • ERBB2
  • ERBB3
  • ERBB4

Assay Format

Pathway:
  • HER/ErbB Signaling
Number of Targets Detected:
17
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
EGFR
(P-Tyr845)
  • Gene Symbol: EGFR
  • Gene ID: 1956
  • Accession #: P00533
EGFR
(P-Tyr992)
EGFR
(P-Tyr1045)
EGFR
(P-Tyr1068)
EGFR
(P-Tyr1086)
EGFR
(P-Tyr1148)
  • Gene Symbol: EGFR
  • Gene ID: 1956
  • Accession #: P00533
EGFR
(P-Tyr1173)
  • Gene Symbol: EGFR
  • Gene ID: 1956
  • Accession #: P00533
EGFR
(P-Ser1046/1047)
  • Gene Symbol: EGFR
  • Gene ID: 1956
  • Accession #: P00533
EGFR
(P-Ser1070)
ErbB2
(P-Tyr877)
  • Gene Symbol: ErbB2
  • Gene ID: 2064
  • Accession #: P04626
ErbB2
(P-Tyr1112)
  • Gene Symbol: ErbB2
  • Gene ID: 2064
  • Accession #: P04626
ErbB2
(P-Tyr 1221/1222)
  • Gene Symbol: ErbB2
  • Gene ID: 2064
  • Accession #: P04626
ErbB2
(P-Tyr1248)
  • Gene Symbol: ErbB2
  • Gene ID: 2064
  • Accession #: P04626
ErbB2
(P-Thr686)
  • Gene Symbol: ErbB2
  • Gene ID: 2064
  • Accession #: P04626
ErbB2
(P-Ser1113)
  • Gene Symbol: ErbB2
  • Gene ID: 2064
  • Accession #: P04626
ErbB3
(P-Tyr1289)
  • Gene Symbol: ErbB3
  • Gene ID: 2065
  • Accession #: P21860
ErbB4
(P-Tyr1284)
  • Gene Symbol: ErbB4
  • Gene ID: 2066
  • Accession #: Q15303

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human EGFR Phosphorylation Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • Protease Inhibitor Cocktail
  • Phosphatase Inhibitor Cocktail Set I
  • Phosphatase Inhibitor Cocktail II
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Wang X., Zhang M., Ping F., et al. Identification and therapeutic intervention of coactivated anaplastic lymphoma kinase, fibroblast growth factor receptor 2, and ephrin type-A receptor 5 kinases in hepatocellular carcinoma. Hepatology. 2018 Jan 21. doi: 10.1002/hep.29792.
    Species: Human
    Sample type: Cell Lysate
  2. Ruiz-Saenz A., Dreyer C., Campbell M., et al. HER2 amplification in tumors activates PI3K/Akt signaling independent of HER3. Cancer Research, epub online 2018, doi: 10.1158/0008-5472.CAN-18-0430
    Species: Human
    Sample type: Cell Lysate
  3. Tang Q., Wang Y., Ma L., et al. Peiminine serves as an adriamycin chemosensitizer in gastric cancer by modulating the EGFR/FAK pathway. Oncol Rep. 2018 Mar;39(3):1299-1305. doi: 10.3892/or.2018.6184.
    Species: Human
    Sample type: Cell Lysate
  4. Asif M., Shafaei A., Abdul Majid A., et al. Mesua ferrea stem bark extract induces apoptosis and inhibits metastasis in human colorectal carcinoma HCT 116 cells, through modulation of multiple cell signalling pathways. Chin J Nat Med. 2017 Jul;15(7):505-514. doi: 10.1016/S1875-5364(17)30076-6.
    Species: Human
    Sample type: Cell Lysate
  5. Kato K., Iwama H., Yamashita T., et al. The anti-diabetic drug metformin inhibits pancreatic cancer cell proliferation in vitro and in vivo: Study of the microRNAs associated with the antitumor effect of metformin. Oncol Rep. 2016 Mar;35(3):1582-92. doi: 10.3892/or.2015.4496
    Species: Human
    Sample type: Tissue Lysate
  6. Mohan N., Shen Y., Endo Y., et al. Trastuzumab, but Not Pertuzumab, Dysregulates HER2 Signaling to Mediate Inhibition of Autophagy and Increase in Reactive Oxygen Species Production in Human Cardiomyocytes. Mol Cancer Ther. 2016 Jun;15(6):1321-31. doi: 10.1158/1535-7163.MCT-15-0741.
    Species: Human
    Sample type: Cell Lysate
  7. Rios-Doria J., Sabol D., Chesebrough J., et al. A Monoclonal Antibody to ADAM17 Inhibits Tumor Growth by Inhibiting EGFR and Non-EGFR-Mediated Pathways. Mol Cancer Ther. 2015 Jul;14(7):1637-49. doi: 10.1158/1535-7163.MCT-14-1040.
    Species: Human
    Sample type: Tissue Lysate
  8. Montermini L., Meehan B., Farnier D., et al. Inhibition of oncogenic epidermal growth factor receptor kinase triggers release of exosome-like extracellular vesicles and impacts their phosphoprotein and DNA content. J Biol Chem. 2015 Oct 2;290(40):24534-46. doi: 10.1074/jbc.M115.679217.
    Species: Human
    Sample type: Extracellular Vesicles
  9. Arnold C., et al. Rac1 as a multifunctional therapeutic target to prevent and combat cancer metastasis. Oncoscience 2014, Vol.1, No.8
    Species: Human
    Sample type: Cell Lysate
  10. Panupinthu N., et al. Self-reinforcing loop of amphiregulin and Y-box binding protein-1 contributes to poor outcomes in ovarian cancer. Oncogene (2014) 33, 2846-2856; doi:10.1038/onc.2013.259
    Species: Human
    Sample type: Cell Lysate
I used this array to test the cell lysates from epidermoid carcinoma cell line exposed to EGF, it works well.
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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