Human Inflammation Array C1

$348.45

RayBio® C-Series Human Inflammation Antibody Array 1 Kit. Detects 20 Human Inflammatory Factors. Suitable for all liquid sample types.

AAH-INF-1-2
+

Antigen Information

Gene Symbol:
CCL1 | CCL11 | CCL24 | CSF2 | CSF3 | CXCL8 | IFNG | IL10 | IL11 | IL12A   View more

Assay Format

Number of Targets Detected:
20
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
4 or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Eotaxin-1
(CCL11)
Eotaxin-2
(MPIF-2/CCL24)
GCSF
GM-CSF
IFN-gamma
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-2
IL-3
IL-4
IL-6
IL-7
IL-8
(CXCL8)
IL-10
IL-11
IL-12 p40
IL-12 p70
IL-13
I-309
(TCA-3/CCL1)
TIMP-2

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Inflammation Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Sanchez-Quesada C., Lopez-Biedma A., Gaforio J. Maslinic Acid enhances signals for the recruitment of macrophages and their differentiation to m1 state. Evid Based Complement Alternat Med. 2015;2015:654721. doi: 10.1155/2015/654721.
    Species: Human
    Sample type: Conditioned Media
  2. Zhao L., Yagiz Y., Xu C., et al. Muscadine grape seed oil as a novel source of tocotrienols to reduce adipogenesis and adipocyte inflammation. Food Funct. 2015 Jul;6(7):2293-302. doi: 10.1039/c5fo00261c.
    Species: Human
    Sample type: Conditioned Media
  3. Maldonado, R., et al. "Cytotoxic effects of Kingella kingae outer membrane vesicles on human cells." Microbial pathogenesis 51.1 (2011): 22-30.
    Species: Human
    Sample type: Conditioned Media
  4. Fernandez A M, Fernandez S, Carrero P, Garcia-Garcia M, Torres-Aleman I. Calcineurin in Reactive Astrocytes Plays a Key Role in the Interplay between Proinflammatory and Anti-Inflammatory Signals.J Neuroscience.2007;27(33):8745-8756
    Species: Human
    Sample type: Cell Lysate
  5. Nakamura Y, Nawata M, Wakitani S. Expression Profiles and Functional Analyses of Wnt-Related Genes in Human Joint Disorders. Am J Pathol. Jul 2005; 167(1): 97-105.
    Species: Human
    Sample type: Conditioned Media
  6. Schriener B, Voss J, Wischhusen J, Dombrowski Y, et al. Expressin of toll-like receptors by human muscle cells in vitro and in vivo: TLR3 is highly expressed in inflammatory and HIV myopathies, mediates IL-8 release and up-regulation of NKG2D ligands. FASEB J. 2005;19(13):U645-U666.
    Species: Human
    Sample type: Conditioned Media

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Note: If you are not placing this order online, make sure to mention this bundle discount to our orders department (orders@raybiotech.com).

Total list price $464.60
Price for all $383.29

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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