Human Inflammation Array C3

RayBio® C-Series Human Inflammation Antibody Array 3 Kit. Detects 40 Human Inflammatory Factors. Suitable for all liquid sample types.

$440.36
AAH-INF-3-2
+

Antigen Information

Gene Symbols:
CCL1 | CCL11 | CCL15 | CCL2 | CCL24 | CCL3 | CCL4 | CCL5 | CCL8 | CSF1   View more

Assay Format

Number of Targets Detected:
40
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
4 or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Eotaxin-1
(CCL11)
Eotaxin-2
(MPIF-2/CCL24)
GCSF
GM-CSF
ICAM-1
(CD54)
IFN-gamma
I-309
(TCA-3/CCL1)
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-2
IL-3
IL-4
IL-6
IL-6 R
IL-7
IL-8
(CXCL8)
IL-10
IL-11
IL-12 p40
IL-12 p70
IL-13
IL-15
IL-16
IL-17A
  • Gene Symbol: IL17A
  • Gene ID: 3605
  • Accession #: Q16552
IP-10
(CXCL10)
MCP-1
(CCL2)
MCP-2
(CCL8)
M-CSF
MIG
(CXCL9)
MIP-1 alpha
(CCL3)
MIP-1 beta
(CCL4)
MIP-1 delta
(CCL15)
RANTES
(CCL5)
TGF beta 1
TNF alpha
TNF beta
(TNFSF1B)
TNF RI
(TNFRSF1A)
TNF RII
(TNFRSF1B)
PDGF-BB
TIMP-2

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Inflammation Antibody Array C3 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP??s ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Drolia R., Tenguria S., Durkes A., et al. Listeria Adhesion Protein Induces Intestinal Epithelial Barrier Dysfunction for Bacterial Translocation. Cell Host Microbe. 2018 Apr 11;23(4):470-484.e7. doi: 10.1016/j.chom.2018.03.004.
    Species: Human
    Sample type: Conditioned Media
  2. Zheng Y., Ji S., Wu H., et al. Topical administration of cryopreserved living micronized amnion accelerates wound healing in diabetic mice by modulating local microenvironment. Biomaterials. 2017 Jan;113:56-67. doi: 10.1016/j.biomaterials.2016.10.031.
    Species: Human
    Sample type: Conditioned Media
  3. Chan J., Tan M., Sng M., et al. Cancer-associated fibroblasts enact field cancerization by promoting extratumoral oxidative stress. Cell Death Dis 8 (1), e2562. 2017 Jan 19.
    Species: Human
    Sample type: Conditioned Media
  4. Xing W., Xiao Y., Lu., et al. GFI1 downregulation promotes inflammation-linked metastasis of colorectal cancer. Cell Death Differ. 2017 May; 24(5): 929–943.
    Species: Human
    Sample type: Conditioned Media
  5. Jalowiec J., D'Este M., Bara J., et al. An In Vitro Investigation of Platelet-Rich Plasma-Gel as a Cell and Growth Factor Delivery Vehicle for Tissue Engineering. Tissue Eng Part C Methods. 2016 Jan;22(1):49-58. doi: 10.1089/ten.TEC.2015.0223.
    Species: Human
    Sample type: Conditioned Media
  6. Pan, Xiao-Ping, et al. "Efficient generation of functional hepatocyte-like cells from mouse liver progenitor cells via indirect co-culture with immortalized human hepatic stellate cells." Hepatobiliary & Pancreatic Diseases International 15.2 (2016): 173-179.
    Species: Human
    Sample type: Conditioned Media
  7. Madhavan PN, Gloria F, Gianna C, Karla M, Agudelo M (2015) Alcohol Versus Cannabinoids: A Review of Their Opposite Neuro-Immunomodulatory Effects and Future Therapeutic Potentials. J Alcohol Drug Depend 3:184. doi: 10.4172/2329-6488.1000184
    Species: Human
    Sample type: Cell Lysate
  8. Rooney P., Srivastava A., Watson L., et al. Hyaluronic acid decreases IL-6 and IL-8 secretion and permeability in an inflammatory model of interstitial cystitis. Acta Biomater. 2015 Jun;19:66-75. doi: 10.1016/j.actbio.2015.02.030.
    Species: Human
    Sample type: Cell Lysate
  9. Muller C., Tufa D., Chatterjee D., et al. The TLR-2/TLR-6 agonist macrophage-activating lipopeptide-2 augments human NK cell cytotoxicity when PGE2 production by monocytes is inhibited by a COX-2 blocker. Cancer Immunol Immunother. 2015 Sep;64(9):1175-84. doi: 10.1007/s00262-015-1723-3.
    Species: Human
    Sample type: Conditioned Media
  10. Huggins C., Pearce S., Peri F., et al. A novel small molecule TLR4 antagonist (IAXO-102) negatively regulates non-hematopoietic toll like receptor 4 signalling and inhibits aortic aneurysms development. Atherosclerosis. 2015 Oct;242(2):563-70. doi: 10.1016/j.atherosclerosis.2015.08.010
    Species: Human
    Sample type: Tissue Lysate

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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