Human MMP Array C1

RayBio® C-Series Human Matrix Metalloproteinase Antibody Array 1 Kit. Detects 10 Human MMPs and TIMPs. Suitable for all liquid sample types.

$348.45
AAH-MMP-1-2
+

Antigen Information

Gene Symbols:
  • MMP1
  • MMP10
  • MMP13
  • MMP2
  • MMP3
  • MMP8
  • MMP9
  • TIMP1
  • TIMP2
  • TIMP4

Assay Format

Pathway:
  • Akt Signaling
  • HIF-1 alpha Signaling
Number of Targets Detected:
10
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
4 or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
MMP-1
MMP-2
MMP-3
MMP-8
MMP-9
MMP-10
MMP-13
TIMP-1
TIMP-2
TIMP-4

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Matrix Metalloproteinase Antibody Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Differential secretome analysis of cancer-associated fibroblasts and bone marrow-derived precursors to identify microenvironmental regulators of colon cancer progression
    Species: Human
    Sample type: Conditioned Media
  2. Lisboa RA, Andrade MV, Cunha-Melo. Toll-like receptor activation and mechanical force stimulation promote the secretion of matrix metalloproteinases 1, 3 and 10 of human periodontal fibroblasts via p38, JNK and NF-kB. Arch Oral Biol. 2013;58(6):731-739.
    Species: Human
    Sample type: Conditioned Media
  3. PAR2-induced inflammatory responses in human kidney tubular epithelial cells
    Species: Human
    Sample type: Conditioned Media
  4. Regenerative Potential of Decellularized Porcine Nucleus Pulposus Hydrogel Scaffolds: Stem Cell Differentiation, Matrix Remodeling, and Biocompatibility Studies
    Species: Human
    Sample type: Conditioned Media
  5. Vesey DA, Suen JY, Seow V, et al. PAR2-induced inflammatory responses in human kidney tubular epithelial cells. Am J Physiol Renal Physiol. 2013 Mar 15;304(6):F737-F750.
    Species: Human
    Sample type: Conditioned Media
  6. Yang B., et al. Chrysin inhibits metastatic potential of human triple-negative breast cancer cells by modulating matrix metalloproteinase-10, epithelial to mesenchymal transition, and PI3K/Akt signaling pathway. J Appl Toxicol. 2014 Jan;34(1):105-12. doi: 10.1002/jat.2941. Epub 2013 Oct 10
    Species: Human
    Sample type: Cell Lysate
  7. Zhu S., Hong J., Tripathi M., Sehdev V., et al. Regulation of CXCR4-Mediated Invasion by DARPP-32 in Gastric Cancer Cells. Mol Cancer Res January 2013 11; 86
    Species: Human
    Sample type: Conditioned Media
  8. Lan, Yu-Yan, et al. "Epstein-Barr virus Zta upregulates matrix metalloproteinases 3 and 9 that synergistically promote cell invasion in vitro." PloS one 8.2 (2013): e56121.
    Species: Human
    Sample type: Conditioned Media
  9. Rhim, Eun-Mi, et al. "Stimulation of Matrix Metalloproteinases by Tumor Necrosis Factor–? in Human Pulp Cell Cultures." Journal of endodontics 39.6 (2013): 795-800.
    Species: Human
    Sample type: Cell Lysate
  10. Filippov, Sergey, et al. "ETC-1002 regulates immune response, leukocyte homing, and adipose tissue inflammation via LKB1-dependent activation of macrophage AMPK." Journal of lipid research 54.8 (2013): 2095-2108.
    Species: Human
    Sample type: Conditioned Media
In our experiment we are greatly satisfied with your kits. We were able to get clear and vivid pictures of MMP's and compare dots for different samples.

Stony Brook University (SUNY/Stony Brook Department of Ob/Gyn)
We have recently used the C-series array for the human MMPs on denatured protein isolated from skin extracts and it works great.
I like to emphasize that even though the kit recommends the use of non-denatured proteins using their supplied buffer, this worked fine for us, which a plus point to use this array.
The customer service and the scientific support team is excellent.
We will definitely use their products again.
Arup
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Analysis Tool Bundle Discount

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Note: If you are not placing this order online, make sure to mention this bundle discount to our orders department (orders@raybiotech.com).

Total list price $464.60
Price for all $383.29

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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