Human MMP Array C1

RayBio® C-Series Human Matrix Metalloproteinase Antibody Array 1 Kit. Detects 10 Human MMPs and TIMPs. Suitable for all liquid sample types.

$348.45
AAH-MMP-1-2
+

Antigen Information

Gene Symbols:
  • MMP1
  • MMP10
  • MMP13
  • MMP2
  • MMP3
  • MMP8
  • MMP9
  • TIMP1
  • TIMP2
  • TIMP4

Assay Format

Pathway:
  • Akt Signaling
  • HIF-1 alpha Signaling
Number of Targets Detected:
10
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
4 or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
MMP-1
MMP-2
MMP-3
MMP-8
MMP-9
MMP-10
MMP-13
TIMP-1
TIMP-2
TIMP-4

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Matrix Metalloproteinase Antibody Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. de Seny, Dominique, et al. "Acute-phase serum amyloid a in osteoarthritis: regulatory mechanism and proinflammatory properties." PloS one 8.6 (2013): e66769.
    Species: Human
    Sample type: Conditioned Media
  2. Jones, Brian A., et al. "Role of ADAM?17, p38 MAPK, Cathepsins, and the Proteasome Pathway in the Synthesis and Shedding of Fractalkine/CX3CL1 in Rheumatoid Arthritis." Arthritis & Rheumatology 65.11 (2013): 2814-2825.
    Species: Human
    Sample type: Conditioned Media
  3. Rajshankar, Dhaarmini, et al. "Role of PTP? in the destruction of periodontal connective tissues." PloS one 8.8 (2013): e70659.
    Species: Human
    Sample type: Conditioned Media
  4. Chellaiah, Meenakshi A., and Tao Ma. "Membrane localization of membrane type 1 matrix metalloproteinase by CD44 regulates the activation of pro-matrix metalloproteinase 9 in osteoclasts." BioMed research international 2013 (2013).
    Species: Mouse
    Sample type: Conditioned Media
  5. Caino MC., Lopez-Haber C., Kissil JL., Kazanietz MG. Non-small cell lung carcinoma cell motility, rac activation and metastatic dissemination are mediated by protein kinase C epsilon. PLoS One. 2012;7(2):e31714. doi: 10.1371/journal.pone.0031714
    Species: Human
    Sample type: Conditioned Media
  6. Connolly M., Mullan RH., McCormick J., et al. Acute-phase serum amyloid A regulates tumor necrosis factor ? and matrix turnover and predicts disease progression in patients with inflammatory arthritis before and after biologic therapy. Arthritis Rheum. 2012 Apr;64(4):1035-45. doi: 10.1002/art.33455. Epub 2011 Nov 10.
    Species: Human
    Sample type: Conditioned Media
  7. Li R, Ackerman WE, Mihai C, Volakis LI, Ghadiali S, Kniss DA. Myoferlin Depletion in Breast Cancer Cells Promotes Mesenchymal to Epithelial Shape Change and Stalls Invasion. Hong W, ed. PLoS ONE 2012;7(6):e39766. doi:10.1371/journal.pone.0039766.
    Species: Human
    Sample type: Conditioned Media
  8. Abdul Muneer P., Alikunju S., Szlachetka A., Haorah J. The mechanisms of cerebral vascular dysfunction and neuroinflammation by MMP-mediated degradation of VEGFR-2 in alcohol ingestion. Arterioscler Thromb Vasc Biol. 2012 May;32(5):1167-77. doi: 10.1161/ATVBAHA.112.247668
    Species: Human
    Sample type: Conditioned Media
  9. Ranzato E., Martinotti S., Burlando B., et al.
    Species: Human
    Sample type: Conditioned Media
  10. Kummer N., Nowicki T., Azzi J., et al. Arachidonate 5 lipoxygenase expression in papillary thyroid carcinoma promotes invasion via MMP-9 induction. J Cell Biochem. 2012 Jun;113(6):1998-2008. doi: 10.1002/jcb.24069.
    Species: Human
    Sample type: Conditioned Media
In our experiment we are greatly satisfied with your kits. We were able to get clear and vivid pictures of MMP's and compare dots for different samples.

Stony Brook University (SUNY/Stony Brook Department of Ob/Gyn)
We have recently used the C-series array for the human MMPs on denatured protein isolated from skin extracts and it works great.
I like to emphasize that even though the kit recommends the use of non-denatured proteins using their supplied buffer, this worked fine for us, which a plus point to use this array.
The customer service and the scientific support team is excellent.
We will definitely use their products again.
Arup
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Analysis Tool Bundle Discount

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Note: If you are not placing this order online, make sure to mention this bundle discount to our orders department (orders@raybiotech.com).

Total list price $464.60
Price for all $383.29

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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