Human MMP Array C1

RayBio® C-Series Human Matrix Metalloproteinase Antibody Array 1 Kit. Detects 10 Human MMPs and TIMPs. Suitable for all liquid sample types.

$348.45  
AAH-MMP-1-2
+

Antigen Information

Gene Symbols:
  • MMP1
  • MMP10
  • MMP13
  • MMP2
  • MMP3
  • MMP8
  • MMP9
  • TIMP1
  • TIMP2
  • TIMP4

Assay Format

Pathway:
  • Akt Signaling
  • HIF-1 alpha Signaling
Number of Targets Detected:
10
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
4 or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
MMP-1
MMP-2
MMP-3
MMP-8
MMP-9
MMP-10
MMP-13
TIMP-1
TIMP-2
TIMP-4

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Matrix Metalloproteinase Antibody Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Santosh N., et al. Matrix Metalloproteinase Expression During Blastema Formation in Regeneration-Competent Versus Regeneration-Deficient Amphibian Limbs. DEVELOPMENTAL DYNAMICS 240:1127-1141, 2011. DOI 10.1002/dvdy.22503
    Species: Xenopus
    Sample type: Tissue Lysate
  2. Puig?Costa, Manuel, et al. "Antibody microarray?based technology to rapidly define matrix metalloproteinase (MMP) signatures in patients undergoing resection for primary gastric carcinoma." Journal of surgical oncology 104.1 (2011): 106-109.
    Species: Human
    Sample type: Tissue lysate
  3. Gacchina, Carmen, Thomas Brothers, and Anand Ramamurthi. "Evaluating smooth muscle cells from CaCl2-induced rat aortal expansions as a surrogate culture model for study of elastogenic induction of human aneurysmal cells." Tissue Engineering Part A 17.15-16 (2011): 1945-1958.
    Species: Human
    Sample type: Conditioned Media
  4. Miya, Masaaki, et al. "Enhancement of in vitro human tubulogenesis by endothelial cell-derived factors: implications for in vivo tubular regeneration after injury." American Journal of Physiology-Renal Physiology 301.2 (2011): F387-F395.
    Species: Human
    Sample type: Conditioned Media
  5. Harper JI, Godwin H, Green A, Wilkes LE, Holden NJ, et al. A study of matrix metalloproteinase expression and activity in atopic dermatitis using a novel skin wash sampling assay for functional biomarker analysis. Br J Dermatol. 2010 Feb 1;162(2):397-403.
    Species: Human
    Sample type: Skin Wash
  6. Raghu H., Lakka S., Gondi C., Mohanam S., et al. Suppression of uPA and uPAR Attenuates Angiogenin Mediated Angiogenesis in Endothelial and Glioblastoma Cell Lines. PLOS One, Published: August 27, 2010. DOI: 10.1371/journal.pone.0012458
    Species: Human
    Sample type: Conditioned Media
  7. Steed D, Trumpower C, Duffy D, Smith C, Marshall V, et al. Amnion-derived Cellular Cytokine Solution A Physiological Combination of Cytokines for Wound Healing. Eplasty. 2008;8:e18.
    Species: Human
    Sample type: Conditioned Media
  8. Thielitz A, Vetter R, Schultze B, Wrenger S, Simeoni L,et al.Inhibitors of Dipeptidyl Peptidase IV-Like Activity Mediate Antifibrotic Effects in Normal and Keloid-Derived Skin Fibroblasts. J Investig Dermatol. 2008;128:855-866
    Species: Human
    Sample type: Conditioned Media
  9. Cortez DM, Feldman MD, Mummidi S, Valente AJ, Steffensen B, et al. IL-17 stimulates MMP-1 expression in primary human cardiac fibroblasts via p38 MAPK- and ERK1/2-dependent C/EBP-beta, NF-kB, and AP-1 activation. Am J Physiol Heart Circ Physiol. 2007;293:H3356-H3365.
    Species: Human
    Sample type: Conditioned Media
  10. Del Carlo M, Schwartz D, Erickson EA, Loeser RF. Endogenous production of reactive oxygen species is required for stimulstion of human articular chondrocyte matrix metalloproteinase production by fibronectin fragments. Free Radical Biol Med. 2007;42:1350-1358.
    Species: Human
    Sample type: Conditioned Media
In our experiment we are greatly satisfied with your kits. We were able to get clear and vivid pictures of MMP's and compare dots for different samples.

Stony Brook University (SUNY/Stony Brook Department of Ob/Gyn)
We have recently used the C-series array for the human MMPs on denatured protein isolated from skin extracts and it works great.
I like to emphasize that even though the kit recommends the use of non-denatured proteins using their supplied buffer, this worked fine for us, which a plus point to use this array.
The customer service and the scientific support team is excellent.
We will definitely use their products again.
Arup
Write A Review

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

C-Series Testing Service C-Series Testing Service

Amazon Gift Card Program

Customers Also Bought Customers Also Bought