Human MMP Array C1

RayBio® C-Series Human Matrix Metalloproteinase Antibody Array 1 Kit. Detects 10 Human MMPs and TIMPs. Suitable for all liquid sample types.

AAH-MMP-1-2
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Antigen Information

Gene Symbols:
  • MMP1
  • MMP10
  • MMP13
  • MMP2
  • MMP3
  • MMP8
  • MMP9
  • TIMP1
  • TIMP2
  • TIMP4

Assay Format

Pathway:
  • Akt Signaling
  • HIF-1 alpha Signaling
Number of Targets Detected:
10
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
4 or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
MMP-1
MMP-2
MMP-3
MMP-8
MMP-9
MMP-10
MMP-13
TIMP-1
TIMP-2
TIMP-4

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Matrix Metalloproteinase Antibody Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
In our experiment we are greatly satisfied with your kits. We were able to get clear and vivid pictures of MMP's and compare dots for different samples.

Stony Brook University (SUNY/Stony Brook Department of Ob/Gyn)
We have recently used the C-series array for the human MMPs on denatured protein isolated from skin extracts and it works great.
I like to emphasize that even though the kit recommends the use of non-denatured proteins using their supplied buffer, this worked fine for us, which a plus point to use this array.
The customer service and the scientific support team is excellent.
We will definitely use their products again.
Arup
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Analysis Tool Bundle Discount

70% off the data analysis tool when you purchase the array and tool together!


Note: If you are not placing this order online, make sure to mention this bundle discount to our orders department (orders@raybiotech.com).

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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