Human RTK Phosphorylation Array C1

RayBio® C-Series Human Receptor Tyrosine Kinase Phosphorylation Antibody Array 1 Kit. Detects 71 Human RTKs. Suitable for all liquid sample types but intended for use with cell and tissue lysates.

$377.63  
AAH-PRTK-1-2
+

Antigen Information

Gene Symbols:
ABL1 | AXL | BLK | BMX | BTK | CSF1R | CSK | DDR2 | EGFR | EPHA1   View more

Assay Format

Pathway:
  • PI3K-AKT Signaling
Number of Targets Detected:
71
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
ABL1
  • Gene Symbol: ABL1
  • Gene ID: 25
  • Accession #: P00519
ACK
ALK-1
Axl
Blk
BMX
Btk
Csk
Dtk
EGFR
EphA1
EphA2
EphA3
EphA4
EphA5
EphA6
EphA7
EphA8
EphB1
EphB2
EphB3
EphB4
EphB6
ErbB2
ErbB3
ErbB4
FAK
FER
FGFR1
FGFR2
FGFR2 alpha isoform
  • Gene Symbol: FGFR2
  • Gene ID: 2263
  • Accession #: P21802
Fgr
FRK
Fyn
Hck
HGFR
IGF-1 R
Insulin R
(CD220)
Itk
JAK1
JAK2
JAK3
LCK
LTK
Lyn
MATK
M-CSF R
MUSK
NGFR
(TNFRSF16)
PDGFRA
PDGFRB
PYK2
RET
ROR1
ROR2
ROS
RYK
SCF R
(CD117/c-kit)
SRMS
SYK
Tec
Tie-1
Tie-2
TNK1
TRKB
TXK
Tyk2
TYRO10
(DDR2/TKT)
VEGFR2
VEGFR3
ZAP70

Species Detected

Human

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human RTK Phosphorylation Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Anti-Phospho Tyrosine Detection Antibody
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer (1 x 5 ml)
  • Protease Inhibitor Cocktail
  • Phosphatase Inhibitor Cocktail II
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP??s ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Li J, Sipple J, Maynard S, et al. Fanconi Anemia Links Reactive Oxygen Species to Insulin Resistance and Obesity. Antioxidants & Redox Signaling 2012;17(8):1083-1098. doi:10.1089/ars.2011.4417.
    Species: Human
    Sample type: Cell Lysate
  2. Shimokawa T, Seike M, Soeno C, et al. Enzastaurin has anti-tumour effects in lung cancers with overexpressed JAK pathway molecules. British Journal of Cancer 2012;106(5):867-875. doi:10.1038/bjc.2012.7.
    Species: Human
    Sample type: Cell Lysate
  3. Zhang H., Pelech S. Using protein microarrays to study phosphorylation-mediated signal transduction. Semin Cell Dev Biol. 2012 Oct;23(8):872-82. doi: 10.1016/j.semcdb.2012.05.009
    Species: Human
    Sample type: N/A
  4. Eisenberg M., et al. Mechanistic modeling of the effects of myoferlin on tumor cell invasion. PNAS December 13, 2011. vol. 108 no. 50 20078-20083, doi:10.1073/pnas.1116327108
    Species: Human
    Sample type: Cell Lysate
  5. Liu S., et al. Use of protein array technology to investigate receptor tyrosine kinases activated in hepatocellular carcinoma. Exp Therapeutic Med. May 2011Volume 2 Issue 3 Print ISSN: 1792-0981Online ISSN:1792-1015. 10.3892/etm.2011.215
    Species: Human
    Sample type: Tissue Lysate
  6. Zueva E., et al. Metastasis-focused cell-based SELEX generates aptamers inhibiting cell migration and invasion. Int J Cancer. 2011 15 February;128(4):797-804.
    Species: Hamster
    Sample type: Cell Lysate
  7. Perng, Diahn-Warng, et al. "Matrix metalloprotease-9 induces transforming growth factor-? 1 production in airway epithelium via activation of epidermal growth factor receptors."?Life sciences?89.5 (2011): 204-212.
    Species: Human
    Sample type: Cell Lysate
  8. Fischer I, Schulze S, Kuhn C, Friese K, Walzel H, Markert UR, Jeschke U. Inhibiton of RET and JAK2 Signals and Upregulation of VEGFR3 Phosphorylation in Vitro by Galectin-1 in Trophoblast Tumor Cells BeWo. Placenta 2009:30:1078-1082.
    Species: Human
    Sample type: Cell Lysate
  9. Guerra-Laso, José M., et al. "Macrophages from elders are more permissive to intracellular multiplication of Mycobacterium tuberculosis."?Age?35.4 (2013): 1235-1250.
    Species: Human
    Sample type: Cell Lysate
  10. Tirado-González, I., et al. "Galectin-1 influences trophoblast immune evasion and emerges as a predictive factor for the outcome of pregnancy."?Molecular human reproduction?19.1 (2013): 43.
    Species: Human
    Sample type: Cell Lysate
Variation between arrays is very limited, so comparison between experimental and control can be done accurately. Duplicate spots in a array come out nicely and equally. It would be nice to have a serial number printed on the membrane if it is possible.

Columbia University
Kit worked pretty well. It would be nice if the rows and columns were labelled somehow. It was somewhat difficult to to determine which row was which if two rows next to each other had no RTKs with signal. Protocol was easy to follow.

Michigan State University
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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