Mouse Angiogenesis Array C1

RayBio® C-Series Mouse Angiogenesis Antibody Array 1 Kit. Detects 24 Mouse Angiogenic Factors. Suitable for all liquid sample types.

$375.72
AAM-ANG-1-2
+

Antigen Information

Gene Symbols:
CCL11 | CCL2 | CSF1 | CSF2 | CSF3 | CXCL9 | FASLG | FGF2 | IFNG | IGF2   View more

Assay Format

Number of Targets Detected:
24
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
4 or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Eotaxin-1
(CCL11)
Fas Ligand
(TNFSF6)
bFGF
GCSF
GM-CSF
IFN-gamma
IGF-2
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-12 p40/p70
IL-12 p70
  • Gene Symbol: IL12A
  • Accession #: P43431
IL-13
IL-6
IL-9
Leptin
MCP-1
(CCL2)
M-CSF
MIG
(CXCL9)
Platelet Factor 4
(CXCL4)
TIMP-1
TIMP-2
TNF alpha
Thrombopoietin
(TPO)
VEGF-A

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Mouse Angiogenesis Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Zhu L., Cheng X., Shi J., et al. Crosstalk between bone marrow-derived myofibroblasts and gastric cancer cells regulates cancer stemness and promotes tumorigenesis. Oncogene advance online publication 25 April 2016; doi: 10.1038/onc.2016.76
    Species: Mouse
    Sample type: Conditioned Media
  2. Mabeta P., Pepper M. Altered expression of platelet factor 4 and basic fibroblast growth factor correlates with the inhibition of tumor growth in mice. Biomedicine & Pharmacotherapy 69 (2015) 186-190
    Species: Mouse
    Sample type: Plasma
  3. Adini I., Adini A., Bazinet L., et al. Melanocyte pigmentation inversely correlates with MCP-1 production and angiogenesis-inducing potential. FASEB J. 2014 Nov 18. pii: fj.14-255398.
    Species: Mouse
    Sample type: Conditioned Media
  4. Alupei MC., Licarete E., Patra L., Banciu M. Liposomal simvastatin inhibits tumor growth via targeting tumor-associated macrophages-mediated oxidative stress. Cancer Lett. 2015 Jan 28;356(2 Pt B):946-52. doi: 10.1016/j.canlet.2014.11.010.
    Species: Mouse
    Sample type: Tissue Lysate
  5. Zhang J., et al. Chemopreventive Effect of Korean Angelica Root Extract on TRAMP Carcinogenesis and Integrative “Omic” Profiling of Affected Neuroendocrine Carcinomas. Molecular Carcinogenesis October 2014, epub ahead of print. DOI 10.1002/mc.22230
    Species: Mouse
    Sample type: Cell Lysate
  6. Schaafhausen, Maximilian K., et al. "Tumor angiogenesis is caused by single melanoma cells in a manner dependent on reactive oxygen species and NF-?B." J Cell Sci 126.17 (2013): 3862-3872.
    Species: Mouse
    Sample type: Conditioned Media
  7. Giddabasappa A., Eswaraka J., Barrett C., et al. ?-LGND2, an ER? selective agonist, inhibits pathologic retinal neovascularization. Invest Ophthalmol Vis Sci. 2012 Jul 31;53(8):5066-75. doi: 10.1167/iovs.12-9627.
    Species: Mouse
    Sample type: Tissue Lysate
  8. Huang H., Haq O., Utsumi T., et al. Intestinal and plasma VEGF levels in cirrhosis: the role of portal pressure. J Cell Mol Med. 2012 May;16(5):1125-33. doi: 10.1111/j.1582-4934.2011.01399.x.
    Species: Rat
    Sample type: Plasma
  9. Caso G., et al. Dysregulation of CXCL9 and reduced tumor growth in Egr-1 deficient mice. J Hematol Oncol. 2009 Feb 7;2:7. doi: 10.1186/1756-8722-2-7.
    Species: Mouse
    Sample type: Tissue Lysate

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Total list price $491.87
Price for all $410.56

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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