Mouse Cytokine Array C1

RayBio® C-Series Mouse Cytokine Antibody Array 1 Kit. Detects 22 Mouse Cytokines. Suitable for all liquid sample types.

$342.39
AAM-CYT-1-2
+

Antigen Information

Gene Symbols:
CCL12 | CCL2 | CCL5 | CSF2 | CSF3 | IFNG | IL10 | IL12A | IL13 | IL17A   View more

Assay Format

Number of Targets Detected:
22
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
GCSF
GM-CSF
IL-2
IL-3
IL-4
IL-5
IL-6
IL-9
IL-10
IL-12 p40/p70
IL-12 p70
  • Gene Symbol: IL12A
  • Accession #: P43431
IL-13
IL-17A
IFN-gamma
MCP-1
(CCL2)
MCP-5
RANTES
(CCL5)
SCF
TNF RI
(TNFRSF1A)
TNF alpha
Thrombopoietin
(TPO)
VEGF-A

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Mouse Cytokine Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Grum-Schwensen B., Klingelhofer J., Beck M., et al. S100A4-neutralizing antibody suppresses spontaneous tumor progression, pre-metastatic niche formation and alters T-cell polarization balance. BMC Cancer. 2015; 15: 44. doi: 10.1186/s12885-015-1034-2
    Species: Mouse
    Sample type: Conditioned Media
  2. Kim B, Kim JE, Choi B-K, Kim H-S. Anti-Inflammatory Effects of Water Chestnut Extract on Cytokine Responses via Nuclear Factor-?B-signaling Pathway. Biomolecules & Therapeutics 2015;23(1):90-97. doi:10.4062/biomolther.2014.080.
    Species: Mouse
    Sample type: Conditioned Media
  3. Wisnewski AV., Liu J., Colangelo CM. Glutathione reaction products with a chemical allergen, methylene-diphenyl diisocyanate, stimulate alternative macrophage activation and eosinophilic airway inflammation. Chem Res Toxicol. 2015 Apr 20;28(4):729-37. doi: 10.1021/tx5005002.
    Species: Mouse
    Sample type: BAL Fluid
  4. Yang H., Wang H., Ju Z., et al. MD-2 is required for disulfide HMGB1-dependent TLR4 signaling. J Exp Med. 2015 Jan 12;212(1):5-14. doi: 10.1084/jem.20141318.
    Species: Mouse
    Sample type: Conditioned Media
  5. Collignon A., Perles-Barbacaru A., Robert S., et al. A pancreatic tumor-specific biomarker characterized in humans and mice as an immunogenic onco-glycoprotein is efficient in dendritic cell vaccination. Oncotarget. 2015 Sep 15;6(27):23462-79.
    Species: Mouse
    Sample type: Conditioned Media
  6. Hsu Y, Hsu J., Lin K. Yam storage protein dioscorins modulate cytokine gene expression in BALB/c and C57BL/6 lymphocytes. Food and Agricultural Immunology Volume 26, 2015 - Issue 6, 909-923. http://dx.doi.org/10.1080/09540105.2015.1048787
    Species: Mouse
    Sample type: Conditioned Media
  7. Kobayashi T., Tanaka K., Fujita T., et al. Bidirectional role of IL-6 signal in pathogenesis of lung fibrosis. Respir Res. 2015 Aug 20;16:99. doi: 10.1186/s12931-015-0261-z
    Species: Mouse
    Sample type: Conditioned Media
  8. Hoffmann J., Otte A., Thiele S., et al. Sex differences in H7N9 influenza A virus pathogenesis. Vaccine. 2015 Dec 8;33(49):6949-54. doi: 10.1016/j.vaccine.2015.08.044
    Species: Mouse
    Sample type: Tissue Lysate
  9. Carnevalli L., Scognamiglio R., Cabezas-Wallschied N., Rahmig S., Laurenti E. Improved HSC reconstitution and protection from inflammatory stress and chemotherapy in mice lacking granzyme B. JEM vol. 211 no. 5 769-779
    Species: Mouse
    Sample type: Conditioned Media
  10. Chae M., Peterson TE., Balgeman A., et al. Increasing glioma-associated monocytes leads to increased intratumoral and systemic myeloid-derived suppressor cells in a murine model. Neuro Oncol. 2014 Dec 23. pii: nou343. [Epub ahead of print]
    Species: Mouse
    Sample type: Conditioned Media
Thank you for the product. It worked really well with our samples. I used overnight incubation and I am happy with the result. One of our labmates have ordered another Cytokine array after seeing it working for me.

Albert Einstein College of Medicine
clear spots, convenient to use, very good product with high quality and relatively low price.
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Analysis Tool Bundle Discount

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1 x $342.39
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70% off the data analysis tool when you purchase the array and tool together!


Note: If you are not placing this order online, make sure to mention this bundle discount to our orders department (orders@raybiotech.com).

Total list price $458.54
Price for all $377.23

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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