Mouse Cytokine Array C2

RayBio® C-Series Mouse Cytokine Antibody Array 2 Kit. Detects 32 Mouse Cytokines. Suitable for all liquid sample types.

$440.36
AAM-CYT-2-2
+

Antigen Information

Gene Symbols:
CCL11 | CCL12 | CCL17 | CCL19 | CCL2 | CCL20 | CCL21 | CCL27 | CCL3 | CCL5   View more

Assay Format

Number of Targets Detected:
32
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
6Ckine
(CCL21)
CTACK
(CCL27)
Eotaxin-1
(CCL11)
GCSF
GM-CSF
IL-2
IL-3
IL-4
IL-5
IL-6
IL-9
IL-10
IL-12 p40/p70
IL-12 p70
  • Gene Symbol: IL12A
  • Accession #: P43431
IL-13
IL-17A
  • Gene Symbol: IL17A
  • Accession #: Q60971
IFN-gamma
KC
(CXCL1)
Leptin
MCP-1
(CCL2)
MCP-5
MIP-1 alpha
(CCL3)
MIP-2
MIP-3 beta
(CCL19)
RANTES
(CCL5)
SCF
TNF RI
(TNFRSF1A)
TARC
(CCL17)
TIMP-1
TNF alpha
Thrombopoietin
(TPO)
VEGF-A

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Mouse Cytokine Antibody Array C2 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP??s ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Waern I., Karlsson I., Pejler G., Wernersson S. IL?6 and IL?17A degradation by mast cells is mediated by a serglycin:serine protease axis. Immun Inflamm Dis. 2016 Mar; 4(1): 70-79. doi: 10.1002/iid3.95
    Species: Mouse
    Sample type: Conditioned Media
  2. Kim Y., Yang X., Yamashita S., et al. 1,2,3,4,6-penta-O-galloyl-?-D-glucopyranose increases a population of T regulatory cells and inhibits IgE production in ovalbumin-sensitizedmice. Int Immunopharmacol. 2015 May;26(1):30-6. doi: 10.1016/j.intimp.2015.02.025.
    Species: Mouse
    Sample type: Serum
  3. Chu H, Xiang J, Wu P, et al. The role of aquaporin 4 in apoptosis after intracerebral hemorrhage. Journal of Neuroinflammation 2014;11(1):184. doi:10.1186/s12974-014-0184-5.
    Species: Mouse
    Sample type: Tissue Lysate
  4. Ehninger D. Tsc2 Haploinsufficiency Has Limited Effects on Fetal BrainCytokine Levels during Gestational Immune Activation. Autism Research and Treatment. Volume 2014, Article ID 761279, 7 pages http://dx.doi.org/10.1155/2014/761279
    Species: Mouse
    Sample type: Tissue Lysate
  5. Herrmann, A., Priceman, S., et al. CTLA4 aptamer delivers STAT3 siRNA to tumor-associated and malignant T cells. The Journal of Clinical Investigation 2014. Epub Ahead of Print DOI: 10.1172/JCI73174
    Species: Mouse
    Sample type: Conditioned Media
  6. Jung Y., Kim M., Soh H., Lee S., et al. TC1(C8orf4) Regulates Hematopoietic Stem/Progenitor Cells and Hematopoiesis. PLOS One. Published: June 17, 2014 DOI: 10.1371/journal.pone.0100311
    Species: Mouse
    Sample type: Conditioned Media
  7. Shan Y., er al. Suppression of mucin 2 promotes interleukin-6 secretion and tumor growth in an orthotopic immune-competent colon cancer animal model. ONCOLOGY REPORTS 32: 2335-2342, 2014, DOI: 10.3892/or.2014.3544
    Species: Mouse
    Sample type: Conditioned Media
  8. Zhu D., Can C., Purwanti Y., Du S., Lam D., Wu C., et al. Induced Pluripotent Stem Cell-derived Neural Stem Cells Transduced with Baculovirus Encoding CD40 Ligand for Immunogene Therapy in Mouse Models of Breast Cancer. Human Gene Therapy Online Ahead of Editing: April 28th, 2014. doi:10.1089/hum.2013.160. [Epub ahead of print]
    Species: Mouse
    Sample type: Conditioned Media
  9. Suda, Naoto, Keiji Moriyama, and Ganjargal Ganburged. "Effect of angiotensin II receptor blocker on experimental periodontitis in a mouse model of Marfan syndrome." Infection and immunity 81.1 (2013): 182-188.
    Species: Mouse
    Sample type: Serum
  10. Brown NE, Jeselsohn R, Bihani T, et al. Cyclin D1 activity regulates autophagy and senescence in the mammary epithelium. Cancer research 2012;72(24):6477-6489. doi:10.1158/0008-5472.CAN-11-4139.
    Species: Mouse
    Sample type: Tissue Lysate
It's very convenient to use and saves a lot of time for so many cytokines.

Augusta University
It is really good for cell lysates. For the cell cultured media, which possibly have a high background, dilution may be still necessary. (Although no dilution needed in the protocol book).

Temple university
Works well for identifying the target cytokines, including more cytokine than the early series.
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Total list price $556.51
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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