Mouse Cytokine Array C4

RayBio® C-Series Mouse Cytokine Antibody Array 4 Kit. Detects 34 Mouse Cytokines. Suitable for all liquid sample types.

$417.13
AAM-CYT-4-2
+

Antigen Information

Gene Symbols:
CCL22 | CXCL11 | CXCL15 | DPP4 | FCGR2B | FGF2 | FIGF | FLT1 | FLT3LG | FLT4   View more

Assay Format

Number of Targets Detected:
34
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
bFGF
CD26
(DPPIV)
Dtk
E-Selectin
Fc gamma RIIB
(CD32b)
Flt-3 Ligand
GITR
(TNFRSF18)
HGFR
ICAM-1
(CD54)
IGFBP-2
IGF-1
IGF-2
IL-15
IL-17 RB
IL-7
I-TAC
(CXCL11)
Lungkine
(CXCL15)
MDC
(CCL22)
MMP-2
MMP-3
Osteopontin
(SPP1)
Osteoprotegerin
(TNFRSF11B)
Pro-MMP-9
Resistin
Sonic Hedgehog N-Terminal
(Shh-N)
TCK-1
(CXCL7)
TIMP-2
TRANCE
(TNFSF11)
TROY
(TNFRSF19)
TSLP
VEGFR1
VEGFR2
VEGFR3
VEGF-D
  • Gene Symbol: FIGF
  • Accession #: A2AIH3

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Mouse Cytokine Antibody Array C4 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Kashiwagi M., Hosoi J., Lai J., et al. Direct control of regulatory T cells by keratinocytes. Nat Immunol. 2017 Mar;18(3):334-343. doi: 10.1038/ni.3661.
    Species: Mouse
    Sample type: Conditioned Media
  2. Hosaka K., Rojas K., Fazal H., et al. Monocyte Chemotactic Protein-1–Interleukin-6–Osteopontin Pathway of Intra-Aneurysmal Tissue Healing. Stroke. 2017 Apr;48(4):1052-1060. doi: 10.1161/STROKEAHA.116.015590
    Species: Mouse
    Sample type: Tissue Lysate
  3. Wang R., Yu Z., Sunchu B., et al. Rapamycin inhibits the secretory phenotype of senescent cells by a Nrf2-independent mechanism. Aging Cell. 2017 Mar 31. doi: 10.1111/acel.12587
    Species: Mouse
    Sample type: Conditioned Media
  4. Meng X., Vander Ark A., Lee P., et al. Myeloid-specific TGF-? signaling in bone promotes basic-FGF and breast cancer bone metastasis. Oncogene. 2016 May 5;35(18):2370-8. doi: 10.1038/onc.2015.297.
    Species: Mouse
    Sample type: Tissue Lysate
  5. Ivshina M., Alexandrov IM., Vertii A., et al. CPEB Regulation of TAK1 Synthesis Mediates Cytokine Production and the Inflammatory Immune Response. Mol Cell Biol. 2015 Feb 1;35(3):610-8. doi: 10.1128/MCB.00800-14
    Species: Mouse
    Sample type: Serum
  6. Arshad A, Deutsch E, Vozenin M-C (2015) Simultaneous Irradiation of Fibroblasts and Carcinoma Cells Repress the Secretion of Soluble Factors Able to Stimulate Carcinoma Cell Migration. PLoS ONE 10(1): e0115447. doi:10.1371/journal. pone.0115447
    Species: Mouse
    Sample type: Conditioned Media
  7. Grum-Schwensen B., Klingelhofer J., Beck M., et al. S100A4-neutralizing antibody suppresses spontaneous tumor progression, pre-metastatic niche formation and alters T-cell polarization balance. BMC Cancer. 2015; 15: 44. doi: 10.1186/s12885-015-1034-2
    Species: Mouse
    Sample type: Conditioned Media
  8. Yang Y., Gorzelanny C., Bauer AT., et al. Nuclear heparanase-1 activity suppresses melanoma progression via its DNA-binding affinity. Oncogene. 2015 Mar 9. doi: 10.1038/onc.2015.40.
    Species: Mouse
    Sample type: Tissue Lysate
  9. Obenauf A., Zou Y., Ji A., et al. Therapy-induced tumour secretomes promote resistance and tumour progression. Nature. 2015 Apr 16;520(7547):368-72. doi: 10.1038/nature14336.
    Species: Mouse
    Sample type: Conditioned Media
  10. Haldar S., Dru C., Choudhury D., et al. Inflammation and pyroptosis mediate muscle expansion in an IL-1? -dependent manner. The Journal of Biological Chemistry 290, 6574-6583. doi: 10.1074/jbc.M114.617886
    Species: Mouse
    Sample type: Tissue Lysate

No posts found

Write A Review
U.S. Non-U.S.

Analysis Tool Bundle Discount

Specify Size
1 x $417.13
+
1 x $116.15 $34.84

70% off the data analysis tool when you purchase the array and tool together!


Note: If you are not placing this order online, make sure to mention this bundle discount to our orders department (orders@raybiotech.com).

Total list price $533.28
Price for all $451.97

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

Amazon Gift Card Program

C-Series Testing Service C-Series Testing Service

Related Products Related Products