Mouse Cytokine Array C5

RayBio® C-Series Mouse Cytokine Antibody Array 5 Kit. Detects 48 Mouse Cytokines. Suitable for all liquid sample types.

$438.34
AAM-CYT-5-2
+

Antigen Information

Gene Symbols:
ACE | AGER | AREG | CCL21 | CD27 | CD36 | CD40LG | CD70 | CDH1 | CHRD   View more

Assay Format

Number of Targets Detected:
48
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
4-1BB
(TNFRSF9/CD137)
6Ckine
(CCL21)
ACE
ALK-1
Amphiregulin
Cardiotrophin-1
(CT-1)
CD27
(TNFRSF7)
CD27 Ligand
(TNFSF7)
CD36
(SR-B3)
CD40 Ligand
(TNFSF5)
Chordin
CTLA-4
(CD152)
Decorin
DKK-1
E-Cadherin
EGF
Endoglin
(CD105)
Epigen
Epiregulin
Galectin-1
Gas 1
Gas 6
GITR Ligand
(TNFSF18)
Granzyme B
HAI-1
HGF
IL-1 R4
(ST2)
  • Gene Symbol: IL1RL1
  • Accession #: Q05208
IL-11
IL-17B
IL-17E
(IL-25)
IL-17F
IL-1 RA
(IL-1 F3)
IL-2 R alpha
IL-20
IL-21
IL-28A
(IFN-lambda 2)
IL-6 R
JAM-A
(CD321/F11R)
MAdCAM-1
MFG-E8
Neprilysin
Pentraxin-3
(TSG-14)
Prolactin
RAGE
TACI
(TNFRSF13B)
TREM-1
TWEAK
(TNFSF12)
TWEAK R
(TNFRSF12)

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Mouse Cytokine Antibody Array C5 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Wang R., Yu Z., Sunchu B., et al. Rapamycin inhibits the secretory phenotype of senescent cells by a Nrf2-independent mechanism. Aging Cell. 2017 Mar 31. doi: 10.1111/acel.12587
    Species: Mouse
    Sample type: Conditioned Media
  2. Obenauf A., Zou Y., Ji A., et al. Therapy-induced tumour secretomes promote resistance and tumour progression. Nature. 2015 Apr 16;520(7547):368-72. doi: 10.1038/nature14336.
    Species: Mouse
    Sample type: Conditioned Media
  3. Verhoeven, D., Pichichero, M., et al. Divergent Mucosal and Systemic Responses in Children in Response to Acute Otitis Media. Clinical & Experimental Immunology 2014 May Epub Ahead of Print DOI: 10.1111/cei.12389
    Species: Mouse
    Sample type: Nasopharyngeal Fluid
  4. Bobrie A., Krumeich S., Reyal F., et al. Rab27a supports exosome-dependent and -independent mechanisms that modify the tumor microenvironment and can promote tumor progression. Cancer Res. 2012 Oct 1;72(19):4920-30. doi: 10.1158/0008-5472.CAN-12-0925
    Species: Mouse
    Sample type: Conditioned Media
  5. Owens P, Pickup MW, Novitskiy SV, et al. Disruption of bone morphogenetic protein receptor 2 (BMPR2) in mammary tumors promotes metastases through cell autonomous and paracrine mediators. Proceedings of the National Academy of Sciences of the United States of America 2012;109(8):2814-2819. doi:10.1073/pnas.1101139108.
    Species: Mouse
    Sample type: Tissue Lysate
  6. Puig KL, Floden AM, Adhikari R, Golovko MY, Combs CK (2012) Amyloid Precursor Protein and Proinflammatory Changes Are Regulated in Brain and Adipose Tissue in a Murine Model of High Fat Diet-Induced Obesity. PLoS ONE 7(1): e30378. doi:10.1371/journal.pone.0030378
    Species: Mouse
    Sample type: Conditioned Media
  7. Schubert, Kathleen, et al. "Thy?1 (CD90) regulates the extravasation of leukocytes during inflammation." European journal of immunology 41.3 (2011): 645-656.
    Species: Mouse
    Sample type: BAL Fluid

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Note: If you are not placing this order online, make sure to mention this bundle discount to our orders department (orders@raybiotech.com).

Total list price $554.49
Price for all $473.18

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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