Mouse Cytokine Array C6

RayBio® C-Series Mouse Cytokine Antibody Array 6 Kit. Detects 97 Mouse Cytokines. Suitable for all liquid sample types.

AAM-CYT-6-2
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Antigen Information

Gene Symbols:
AREG | AXL | CCL11 | CCL17 | CCL19 | CCL2 | CCL20 | CCL21 | CCL22 | CCL24   View more

Assay Format

Number of Targets Detected:
97
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
6Ckine
(CCL21)
ALK-1
Amphiregulin
Axl
BLC
(CXCL13)
Cardiotrophin-1
(CT-1)
CD27
(TNFRSF7)
CD27 Ligand
(TNFSF7)
CD30
(TNFRSF8)
CD30 Ligand
(TNFSF8)
CD36
(SR-B3)
CTLA-4
(CD152)
CXCL16
Decorin
DKK-1
E-Cadherin
EGF
Eotaxin-1
(CCL11)
Eotaxin-2
(MPIF-2/CCL24)
Epigen
E-Selectin
Fas Ligand
(TNFSF6)
Fc gamma RIIB
(CD32b)
Flt-3 Ligand
Fractalkine
(CX3CL1)
Galectin-1
Gas 6
GCSF
GITR
(TNFRSF18)
GITR Ligand
(TNFSF18)
Granzyme B
HAI-1
HGF
IFN-gamma
IGFBP-5
IGFBP-6
IGF-2
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-1 ra
(IL-1 F3)
IL-2
IL-2 R alpha
IL-3
IL-4
IL-5
IL-6
IL-9
IL-10
IL-11
IL-12 p40
  • Gene Symbol: IL12B
  • Accession #: P43432
IL-12 p70
  • Gene Symbol: IL12A
  • Accession #: P43431
IL-13
IL-15
IL-17A
IL-17 RB
IL-17E
(IL-25)
IL-17F
IL-20
IL-21
I-TAC
(CXCL11)
JAM-A
(CD321/F11R)
KC
(CXCL1)
Leptin
Leptin R
L-Selectin
(CD62L)
Lungkine
(CXCL15)
MAdCAM-1
MCP-1
(CCL2)
MDC
(CCL22)
MFG-E8
MIG
(CXCL9)
MIP-1 alpha
(CCL3)
MIP-1 gamma
MIP-2
MIP-3 alpha
(CCL20)
MIP-3 beta
(CCL19)
MMP-2
Osteopontin
(SPP1)
Osteoprotegerin
(TNFRSF11B)
Prolactin
Pro-MMP-9
RANTES
(CCL5)
SCF
TNF RI
(TNFRSF1A)
TNF RII
(TNFRSF1B)
TACI
(TNFRSF13B)
TARC
(CCL17)
TNF alpha
Thrombopoietin
(TPO)
TRANCE
(TNFSF11)
TROY
(TNFRSF19)
TWEAK R
(TNFRSF12)
VCAM-1
(CD106)
VEGF-A
VEGFR1
VEGFR3
VEGF-D
  • Gene Symbol: FIGF
  • Accession #: A2AIH3

Species Detected

Mouse

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Mouse Cytokine Antibody Array C6 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 4-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Guyot, N., Wartelle, J. et al. Unopposed Cathepsin G, Neutrophil Elastase, and Proteinase 3 Cause Severe Lung Damage and Emphysema. The American Journal of Pathology 2014. Epub Ahead of Print. DOI: 10.1016/j.ajpath.2014.04.015
    Species: Mouse
    Sample type: BAL Fluid
  2. Cook, R. et al. MerTK Inhibition in Tumor Leukocytes Decreases Tumor Growth and Metastasis. J Clin Invest. Aug 1, 2013; 123(8): 3231-3242. DOI:10.1172/JCI67655
    Species: Mouse
    Sample type: Tissue Lysate
  3. Torres S., et al. Proteome Profiling of Cancer-Associated Fibroblasts Identifies Novel Proinflammatory Signatures and Prognostic Markers for Colorectal Cancer. Clin Cancer Res; 19(21) November 1, 2013. DOI: 10.1158/1078-0432.CCR-13-1130
    Species: Mouse
    Sample type: Conditioned Media
  4. Cheng, Kang, et al. "Transplantation of bone marrow-derived MSCs improves cisplatinum-induced renal injury through paracrine mechanisms." Experimental and molecular pathology 94.3 (2013): 466-473.
    Species: Mouse
    Sample type: Conditioned Media
  5. Ey, B., Eyking, A., Klepak, M., Salzman, N. H., Göthert, J. R., Rünzi, M., ... & Cario, E. (2013). Loss of TLR2 worsens spontaneous colitis in MDR1A deficiency through commensally induced pyroptosis. The Journal of Immunology, 190(11), 5676-5688.
    Species: Mouse
    Sample type: Tissue Lysate
  6. Zhu, Xiao-Dong, et al. "Antiangiogenic therapy promoted metastasis of hepatocellular carcinoma by suppressing host-derived interleukin-12b in mouse models." Angiogenesis 16.4 (2013): 809-820.
    Species: Mice
    Sample type: Plasma
  7. Cheng, Kang, et al. "Bone-derived mesenchymal stromal cells from HIV transgenic mice exhibit altered proliferation, differentiation capacity and paracrine functions along with impaired therapeutic potential in kidney injury." Experimental cell research 319.14 (2013): 2266-2274.
    Species: Mouse
    Sample type: Conditioned Media
  8. Ruckh J., Zhao J., Shadrach J., et al. Rejuvenation of regeneration in the aging central nervous system. Cell Stem Cell. 2012 Jan 6;10(1):96-103. doi: 10.1016/j.stem.2011.11.019.
    Species: Mouse
    Sample type: Serum
  9. Chaballe, Linda, et al. "Involvement of placental growth factor in Wallerian degeneration." Glia 59.3 (2011): 379-396.
    Species: Mouse
    Sample type: Tissue lysate

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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