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Home/Search results/Products/Arrays/Antibody Arrays/C-Series/Cytokine Arrays/Mouse Cytokine Array C1000

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Antigen Information

Gene Symbols:

AXL | CCL1 | CCL11 | CCL12 | CCL17 | CCL19 | CCL2 | CCL20 | CCL22 | CCL24 View more

Assay Format

Number of Targets Detected:

Species Detected:

Mouse

Compatible Sample Types:

Cell Culture Supernatants
Cell Lysates
Plasma
Serum
Tissue Lysates

Design Principle:

Sandwich-based

Method of Detection:

Chemiluminescence

Quantitative/Semi-Quantitative:

Semi-Quantitative

Solid Support:

Membrane

Product Specifications

Size:

2, 4, or 8 Sample Kit

Product Features

Easy to use
No specialized equipment needed
Compatible with nearly any liquid sample
Proven technology (many publications)
Highly sensitive (pg/ml)
Sandwich ELISA specificity
Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Axl Gene Symbol: Axl Accession #: Q00993 ELISA Kit: RayBio Mouse Axl ELISA	bFGF Gene Symbol: FGF2 Accession #: P15655 ELISA Kit: RayBio Mouse bFGF ELISA	BLC (CXCL13) Gene Symbol: CXCL13 Accession #: O55038 ELISA Kit: RayBio Mouse BLC ELISA	CD30 Ligand (TNFSF8) Gene Symbol: CD30LG Accession #: P32972 ELISA Kit: RayBio Mouse CD30 L ELISA	CD30 (TNFRSF8) Gene Symbol: CD30 Accession #: Q60846 ELISA Kit: RayBio Mouse CD30 ELISA
CD40 (TNFRSF5) Gene Symbol: CD40 Accession #: Q99NE2 ELISA Kit: RayBio Mouse CD40 ELISA	CRG-2 Gene Symbol: Cxcl10 Accession #: P17515 ELISA Kit: RayBio Mouse CRG-2 ELISA	CTACK (CCL27) Gene Symbol: CCL27 Accession #: Q9Z1X0 ELISA Kit: RayBio Mouse CTACK ELISA	CXCL16 Gene Symbol: CXCL16 Accession #: Q5F2D5 ELISA Kit: RayBio Mouse CXCL16 ELISA	CD26 (DPPIV) Gene Symbol: DPP4 Accession #: P28843 ELISA Kit: RayBio Mouse DPPIV ELISA
Dtk Gene Symbol: TYRO3 Accession #: O09080 ELISA Kit: RayBio Mouse Dtk ELISA	Eotaxin-1 (CCL11) Gene Symbol: CCL11 Accession #: P48298 ELISA Kit: RayBio Mouse Eotaxin-1 ELISA	Eotaxin-2 (MPIF-2/CCL24) Gene Symbol: Ccl24 Accession #: Q9JKC0 ELISA Kit: RayBio Mouse Eotaxin-2 ELISA	E-Selectin Gene Symbol: SELE Accession #: Q00690 ELISA Kit: RayBio Mouse E-Selectin ELISA	Fas Ligand (TNFSF6) Gene Symbol: FASLG Accession #: P41047 ELISA Kit: RayBio Mouse Fas L ELISA
Fc gamma RIIB (CD32b) Gene Symbol: FCGR2B Accession #: P08101 ELISA Kit: RayBio Mouse Fc-gamma-RIIb ELISA	Flt-3 Ligand Gene Symbol: FLT3LG Accession #: P49772 ELISA Kit: RayBio Mouse Flt-3L ELISA	Fractalkine (CX3CL1) Gene Symbol: CX3CL1 Accession #: O35188 ELISA Kit: RayBio Mouse Fractalkine ELISA	GCSF Gene Symbol: CSF3 Accession #: P09920 ELISA Kit: RayBio Mouse G-CSF ELISA	GITR (TNFRSF18) Gene Symbol: TNFRSF18 Accession #: Q9JKR2 ELISA Kit: RayBio Mouse GITR ELISA
GM-CSF Gene Symbol: CSF2 Accession #: P26955 ELISA Kit: RayBio Mouse GM-CSF ELISA	HGFR Gene Symbol: MET Accession #: D3YVY2 ELISA Kit: RayBio Mouse HGF R ELISA	ICAM-1 (CD54) Gene Symbol: ICAM1 Accession #: P13597 ELISA Kit: RayBio Mouse ICAM-1 ELISA	IFN-gamma Gene Symbol: IFNG Accession #: P01580 ELISA Kit: RayBio Mouse IFN-gamma ELISA	IGFBP-2 Gene Symbol: IGFBP2 Accession #: P47877 ELISA Kit: RayBio Mouse IGFBP-2 ELISA
IGFBP-3 Gene Symbol: IGFBP3 Accession #: P47878 ELISA Kit: RayBio Mouse IGFBP-3 ELISA	IGFBP-5 Gene Symbol: IGFBP5 Accession #: Q07079 ELISA Kit: RayBio Mouse IGFBP-5 ELISA	IGFBP-6 Gene Symbol: IGFBP6 Accession #: Q91X24 ELISA Kit: RayBio Mouse IGFBP-6 ELISA	IGF-1 Gene Symbol: IGF1 Accession #: P05018 ELISA Kit: RayBio Mouse IGF-1 ELISA	IGF-2 Gene Symbol: Igf2 Accession #: P09535 ELISA Kit: RayBio Mouse IGF-2 ELISA
IL-1 beta (IL-1 F2) Gene Symbol: IL1B Accession #: P10749 ELISA Kit: RayBio Mouse IL-1 beta ELISA	IL-10 Gene Symbol: IL10 Accession #: Q0VBJ1 ELISA Kit: RayBio Mouse IL-10 ELISA	IL-12 p40/p70 Gene Symbol: IL12A; IL12B Accession #: P43432 ELISA Kit: RayBio Mouse IL-12 p40/70 ELISA	IL-12 p70 Gene Symbol: IL12A Accession #: P43431	IL-13 Gene Symbol: IL13 Accession #: P20109 ELISA Kit: RayBio Mouse IL-13 ELISA
IL-15 Gene Symbol: IL15 Accession #: Q1AHQ7 ELISA Kit: RayBio Mouse IL-15 ELISA	IL-17A Gene Symbol: IL17A Accession #: Q60971	IL-17 RB Gene Symbol: IL17RB Accession #: Q9JIP3 ELISA Kit: RayBio Mouse IL-17B R ELISA	IL-1 alpha (IL-1 F1) Gene Symbol: IL1A Accession #: P01582 ELISA Kit: RayBio Mouse IL-1 alpha ELISA	IL-2 Gene Symbol: IL2 Accession #: Q791T3 ELISA Kit: RayBio Mouse IL-2 ELISA
IL-3 Gene Symbol: IL3 Accession #: P01586 ELISA Kit: RayBio Mouse IL-3 ELISA	IL-3 R beta Gene Symbol: Csf2rb Accession #: P26955 ELISA Kit: RayBio Mouse IL-3 R beta ELISA	IL-4 Gene Symbol: IL4 Accession #: P07750 ELISA Kit: RayBio Mouse IL-4 ELISA	IL-5 Gene Symbol: IL5 Accession #: P04401 ELISA Kit: RayBio Mouse IL-5 ELISA	IL-6 Gene Symbol: IL6 Accession #: P08505 ELISA Kit: RayBio Mouse IL-6 ELISA
IL-7 Gene Symbol: IL7 Accession #: P10168 ELISA Kit: RayBio Mouse IL-7 ELISA	IL-9 Gene Symbol: IL9 Accession #: P15247 ELISA Kit: RayBio Mouse IL-9 ELISA	I-TAC (CXCL11) Gene Symbol: CXCL11 Accession #: Q9JHH5 ELISA Kit: RayBio Mouse I-TAC ELISA	KC (CXCL1) Gene Symbol: CXCL1 Accession #: P12850 ELISA Kit: RayBio Mouse KC ELISA	Leptin Gene Symbol: LEP Accession #: P41160 ELISA Kit: RayBio Mouse Leptin ELISA
Leptin R Gene Symbol: LEPR Accession #: Q61215 ELISA Kit: RayBio Mouse Leptin R ELISA	LIX Gene Symbol: Cxcl5 Accession #: P50228 ELISA Kit: RayBio Mouse LIX ELISA	L-Selectin (CD62L) Gene Symbol: SELL Accession #: Q3TCF3 ELISA Kit: RayBio Mouse L-Selectin ELISA	Lungkine (CXCL15) Gene Symbol: CXCL15 Accession #: Q3UQ15 ELISA Kit: RayBio Mouse CXCL15 ELISA	Lymphotactin (XCL1) Gene Symbol: XCL1 Accession #: P47993 ELISA Kit: RayBio Mouse Lymphotactin ELISA
MCP-1 (CCL2) Gene Symbol: CCL2 Accession #: Q9QYD7 ELISA Kit: RayBio Mouse MCP-1 ELISA	MCP-5 Gene Symbol: Ccl12 Accession #: Q9QYD6 ELISA Kit: RayBio Mouse MCP-5 ELISA	M-CSF Gene Symbol: CSF1 Accession #: P07141 ELISA Kit: RayBio Mouse M-CSF ELISA	MDC (CCL22) Gene Symbol: CCL22 Accession #: O88430 ELISA Kit: RayBio Mouse MDC ELISA	MIG (CXCL9) Gene Symbol: CXCL9 Accession #: P18340 ELISA Kit: RayBio Mouse MIG ELISA
MIP-1 alpha (CCL3) Gene Symbol: CCL3 Accession #: P14096 ELISA Kit: RayBio Mouse MIP-1 alpha ELISA	MIP-1 gamma Gene Symbol: Ccl9 Accession #: Q5QNW2 ELISA Kit: RayBio Mouse MIP-1 gamma ELISA	MIP-2 Gene Symbol: Cxcl2 Accession #: P10889 ELISA Kit: RayBio Mouse MIP-2 ELISA	MIP-3 beta (CCL19) Gene Symbol: CCL19 Accession #: O70460 ELISA Kit: RayBio Mouse MIP-3 beta ELISA	MIP-3 alpha (CCL20) Gene Symbol: CCL20 Accession #: O89093 ELISA Kit: RayBio Mouse MIP-3 alpha ELISA
MMP-2 Gene Symbol: MMP2 Accession #: P33434 ELISA Kit: RayBio Mouse MMP-2 ELISA	MMP-3 Gene Symbol: MMP3 Accession #: P28862 ELISA Kit: RayBio Mouse MMP-3 ELISA	Osteopontin (SPP1) Gene Symbol: SPP1 Accession #: P19008 ELISA Kit: RayBio Mouse OPN ELISA	Osteoprotegerin (TNFRSF11B) Gene Symbol: TNFRSF11B Accession #: O70202 ELISA Kit: RayBio Mouse OPG ELISA	Platelet Factor 4 (CXCL4) Gene Symbol: PF4, CXCL4, SCYB4 Accession #: Q9Z126 ELISA Kit: RayBio Mouse PF-4 ELISA
Pro-MMP-9 Gene Symbol: Mmp9 Accession #: Q80XI8 ELISA Kit: RayBio Mouse pro-MMP-9 ELISA	P-Selectin Gene Symbol: SELP Accession #: Q01102 ELISA Kit: RayBio Mouse P-Selectin ELISA	RANTES (CCL5) Gene Symbol: CCL5 Accession #: P30882 ELISA Kit: RayBio Mouse RANTES ELISA	Resistin Gene Symbol: RETN Accession #: Q99P87 ELISA Kit: RayBio Mouse Resistin ELISA	SCF Gene Symbol: KITLG Accession #: P20826 ELISA Kit: RayBio Mouse SCF ELISA
SDF-1 alpha (CXCL12 alpha) Gene Symbol: CXCL12 Accession #: P40224 ELISA Kit: RayBio Mouse SDF-1 alpha ELISA	Sonic Hedgehog N-Terminal (Shh-N) Gene Symbol: SHH ELISA Kit: RayBio Mouse ShhN ELISA	TNF RI (TNFRSF1A) Gene Symbol: TNFRSF1A Accession #: P25118 ELISA Kit: RayBio Mouse sTNFRI ELISA	TNF RII (TNFRSF1B) Gene Symbol: TNFRSF1B Accession #: O88734 ELISA Kit: RayBio Mouse sTNFRII ELISA	TARC (CCL17) Gene Symbol: CCL17 Accession #: Q9WUZ6 ELISA Kit: RayBio Mouse TARC ELISA
I-309 (TCA-3/CCL1) Gene Symbol: CCL1 Accession #: P14098 ELISA Kit: RayBio Mouse TCA-3 ELISA	TECK (CCL25) Gene Symbol: CCL25 Accession #: O35903 ELISA Kit: RayBio Mouse TECK ELISA	TCK-1 (CXCL7) Gene Symbol: Ppbp Accession #: Q9EQI5 ELISA Kit: RayBio Mouse TCK-1 ELISA	TIMP-1 Gene Symbol: TIMP1 Accession #: P12032 ELISA Kit: RayBio Mouse TIMP-1 ELISA	TIMP-2 Gene Symbol: TIMP2 Accession #: Q6GXA8 ELISA Kit: RayBio Mouse TIMP-2 ELISA
TNF alpha Gene Symbol: TNF Accession #: Q62326 ELISA Kit: RayBio Mouse TNF-alpha ELISA	Thrombopoietin (TPO) Gene Symbol: THPO Accession #: P40226 ELISA Kit: RayBio Mouse TPO ELISA	TRANCE (TNFSF11) Gene Symbol: TNFSF11 Accession #: Q9R1Y0 ELISA Kit: RayBio Mouse TRANCE ELISA	TROY (TNFRSF19) Gene Symbol: TNFRSF19 Accession #: Q80T13 ELISA Kit: RayBio Mouse TROY ELISA	TSLP Gene Symbol: TSLP Accession #: Q9JIE6 ELISA Kit: RayBio Mouse TSLP ELISA
VCAM-1 (CD106) Gene Symbol: VCAM1 Accession #: P29533 ELISA Kit: RayBio Mouse VCAM-1 ELISA	VEGF-A Gene Symbol: VEGFA Accession #: Q00731 ELISA Kit: RayBio Mouse VEGF-A ELISA	VEGFR1 Gene Symbol: Flt1 Accession #: Q61517 ELISA Kit: RayBio Mouse VEGF R1 ELISA	VEGFR2 Gene Symbol: KDR Accession #: Q3UQZ6 ELISA Kit: RayBio Mouse VEGFR-2 ELISA	VEGFR3 Gene Symbol: FLT4 Accession #: P35917 ELISA Kit: RayBio Mouse VEGFR-3 ELISA
VEGF-D Gene Symbol: FIGF Accession #: A2AIH3

Species Detected

Mouse

Application Notes

Suggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process

Kit Components

Mouse Cytokine Antibody Array C1000 Membranes
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated HRP
Detection Buffer C
Detection Buffer D
Lysis Buffer
8-Well Incubation Tray
Plastic Sheets
Array Templates
Manual

Other Materials Required

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It^® or EpiChem II Benchtop Darkroom or GE's ImageQuant^™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Protocol Outline

Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20^°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20^°C and all other reagents undiluted at 4^°C for no more than 3 months.

Ivshina M., Alexandrov IM., Vertii A., et al. CPEB Regulation of TAK1 Synthesis Mediates Cytokine Production and the Inflammatory Immune Response. Mol Cell Biol. 2015 Feb 1;35(3):610-8. doi: 10.1128/MCB.00800-14
Species: Mouse
Sample type: Serum
Arshad A, Deutsch E, Vozenin M-C (2015) Simultaneous Irradiation of Fibroblasts and Carcinoma Cells Repress the Secretion of Soluble Factors Able to Stimulate Carcinoma Cell Migration. PLoS ONE 10(1): e0115447. doi:10.1371/journal. pone.0115447
Species: Mouse
Sample type: Conditioned Media
Grum-Schwensen B., Klingelhofer J., Beck M., et al. S100A4-neutralizing antibody suppresses spontaneous tumor progression, pre-metastatic niche formation and alters T-cell polarization balance. BMC Cancer. 2015; 15: 44. doi: 10.1186/s12885-015-1034-2
Species: Mouse
Sample type: Conditioned Media
Yang Y., Gorzelanny C., Bauer AT., et al. Nuclear heparanase-1 activity suppresses melanoma progression via its DNA-binding affinity. Oncogene. 2015 Mar 9. doi: 10.1038/onc.2015.40.
Species: Mouse
Sample type: Tissue Lysate
Taka E., Mazzio E., Goodman C., et al. Anti-inflammatory effects of thymoquinone in activated BV-2 microglial cells. J Neuroimmunol. 2015 Sep 15;286:5-12. doi: 10.1016/j.jneuroim.2015.06.011.
Species: Mouse
Sample type: Conditioned Media
Jin H., Chen L., Zhang Z., et al. Deletion of angiotensin-converting enzyme 2 exacerbates renal inflammation and injury in apolipoprotein E-deficient mice through modulation of the nephrin and TNF-alpha-TNFRSF1A signaling. J Transl Med. 2015 Aug 6;13:255. doi: 10.1186/s12967-015-0616-8.
Species: Mouse
Sample type: Tissue Lysate
Achyut B., Shankar A., Iskander A., et al. Bone marrow derived myeloid cells orchestrate antiangiogenic resistance in glioblastoma through coordinated molecular networks. Cancer Lett. 2015 Dec 28;369(2):416-26. doi: 10.1016/j.canlet.2015.09.004.
Species: Mouse
Sample type: Tissue Lysate
Braig M., Pallman N., Preukschas M., Steinemann D., Hofmann W., et al. A ?telomere-associated secretory phenotype? cooperates with BCR-ABL to drive malignant proliferation of leukemic cells. 2014 Leukemia, Pubslihed April 1, Epub Ahead of Print, doi: 10.1038/leu.2014.95
Species: Mouse
Sample type: Conditioned Media
Novitskiy et al.: Attenuated transforming growthfactor beta signaling promotes metastasis in a model of HER2mammary carcinogenesis. Breast Cancer Research 2014 16:425.\
Species: Mouse
Sample type: Conditioned Media
Palmi C., et al. Cytoskeletal Regulatory Gene Expression and Migratory Properties of B Cell Progenitors are Affected by the ETV6-RUNX1 Rearrangement Mol Cancer Res molcanres.0056.2014; Published OnlineFirst July 24, 2014; doi: 10.1158/1541-7786.MCR-14-0056-T
Species: Mouse
Sample type: Conditioned Media

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I used this array for one of my researches and it was very helpful in detecting the impacted cytokines. I published the following paper:

Messeha SS, Zarmouh NO, Mendonca P, Kolta MG, Soliman KFA. The attenuating effects of plumbagin on pro-inflammatory cytokine expression in LPS-activated BV-2 microglial cells. Journal of neuroimmunology. 2017. Epub 2017/09/28. https://doi.org/10.1016/j.jneuroim.2017.09.007 PMID: 28950995.

Samia Messeha
Florida A & M University, Tallahassee, Florida 09/18/2020, 09:36 AM

I have been using these arrays for years, and making great discoveries.

Patricia Mendonca
Florida A & M University 06/10/2019, 09:16 PM

It is useful to find out the expression level of multiple cytokines from the same sample at same setting.

Roxan Ara
Augusta University 12/19/2018, 02:24 PM

We ordered 100 customized membrane for 44 cytokines. It works really good.

Mohammad Harun Rashid
Augusta University 12/11/2018, 01:33 PM

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