Mouse Cytokine Array C2000

RayBio® C-Series Mouse Cytokine Antibody Array 2000. A combination of RayBio® Mouse Cytokine Antibody Array C3, C4, & C5 Kit. Detects 144 Mouse Cytokines. Suitable for all liquid sample types.

$2,279.77  
AAM-CYT-2000-8
+

Antigen Information

Gene Symbols:
ACE | AGER | AREG | AXL | CCL1 | CCL11 | CCL12 | CCL17 | CCL19 | CCL2   View more

Assay Format

Number of Targets Detected:
144
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
4-1BB
(TNFRSF9/CD137)
6Ckine
(CCL21)
ACE
ALK-1
Amphiregulin
Axl
bFGF
BLC
(CXCL13)
Cardiotrophin-1
(CT-1)
CD27
(TNFRSF7)
CD27 Ligand
(TNFSF7)
CD30 Ligand
(TNFSF8)
CD30
(TNFRSF8)
CD36
(SR-B3)
CD40
(TNFRSF5)
CD40 Ligand
(TNFSF5)
Chordin
CRG-2
CTACK
(CCL27)
CTLA-4
(CD152)
CXCL16
Decorin
DKK-1
CD26
(DPPIV)
Dtk
E-Cadherin
EGF
Endoglin
(CD105)
Eotaxin-1
(CCL11)
Eotaxin-2
(MPIF-2/CCL24)
Epigen
Epiregulin
E-Selectin
Fas Ligand
(TNFSF6)
Fc gamma RIIB
(CD32b)
Flt-3 Ligand
Fractalkine
(CX3CL1)
Galectin-1
GCSF
GITR
(TNFRSF18)
GITR Ligand
(TNFSF18)
GM-CSF
Granzyme B
Gas 1
Gas 6
HAI-1
HGF
HGFR
ICAM-1
(CD54)
IFN-gamma
IGFBP-2
IGFBP-3
IGFBP-5
IGFBP-6
IGF-1
IGF-2
IL-1 beta
(IL-1 F2)
IL-1 R4
(ST2)
  • Gene Symbol: IL1RL1
  • Accession #: Q05208
IL-10
IL-11
IL-12 p40/p70
IL-12 p70
  • Gene Symbol: IL12A
  • Accession #: P43431
IL-13
IL-15
IL-17A
  • Gene Symbol: IL17A
  • Accession #: Q60971
IL-17B
IL-17 RB
IL-17E
(IL-25)
IL-17F
IL-1 alpha
(IL-1 F1)
IL-1 RA
(IL-1 F3)
IL-2
IL-2 R alpha
IL-20
IL-21
IL-28A
(IFN-lambda 2)
IL-3
IL-3 R beta
IL-4
IL-5
IL-6
IL-6 R
IL-7
IL-9
I-TAC
(CXCL11)
JAM-A
(CD321/F11R)
KC
(CXCL1)
Leptin
Leptin R
LIX
L-Selectin
(CD62L)
Lungkine
(CXCL15)
Lymphotactin
(XCL1)
MAdCAM-1
MCP-1
(CCL2)
MCP-5
M-CSF
MDC
(CCL22)
MFG-E8
MIG
(CXCL9)
MIP-1 alpha
(CCL3)
MIP-1 gamma
MIP-2
MIP-3 beta
(CCL19)
MIP-3 alpha
(CCL20)
MMP-2
MMP-3
Neprilysin
Osteopontin
(SPP1)
Osteoprotegerin
(TNFRSF11B)
Pentraxin-3
(TSG-14)
Platelet Factor 4
(CXCL4)
Prolactin
Pro-MMP-9
P-Selectin
RAGE
RANTES
(CCL5)
Resistin
SCF
SDF-1 alpha
(CXCL12 alpha)
Sonic Hedgehog N-Terminal
(Shh-N)
TNF RI
(TNFRSF1A)
TNF RII
(TNFRSF1B)
TACI
(TNFRSF13B)
TARC
(CCL17)
I-309
(TCA-3/CCL1)
TECK
(CCL25)
TCK-1
(CXCL7)
TIMP-1
TIMP-2
TNF alpha
Thrombopoietin
(TPO)
TRANCE
(TNFSF11)
TREM-1
TROY
(TNFRSF19)
TSLP
TWEAK
(TNFSF12)
TWEAK R
(TNFRSF12)
VCAM-1
(CD106)
VEGF-A
VEGFR1
VEGFR2
VEGFR3
VEGF-D
  • Gene Symbol: FIGF
  • Accession #: A2AIH3

Species Detected

Mouse

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Mouse Cytokine Antibody Array C2000 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP??s ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Wang R., Yu Z., Sunchu B., et al. Rapamycin inhibits the secretory phenotype of senescent cells by a Nrf2-independent mechanism. Aging Cell. 2017 Mar 31. doi: 10.1111/acel.12587
    Species: Mouse
    Sample type: Conditioned Media
  2. Chang WJ., Song LJ, Yi T., et al. Early activated hepatic stellate cell-derived molecules reverse acute hepatic injury. World Journal of Gastroenterology. Published January 2015, Man#14271
    Species: Mouse
    Sample type: Conditioned Media
  3. Obenauf A., Zou Y., Ji A., et al. Therapy-induced tumour secretomes promote resistance and tumour progression. Nature. 2015 Apr 16;520(7547):368-72. doi: 10.1038/nature14336.
    Species: Mouse
    Sample type: Conditioned Media
  4. Files D., Liu C., Seeds M., Morris P., Delbono O. Neutrophilic Lung Injury Is Attenuated By Therapeutic Exercise In Young, But Not Old Mice With Ards. Am J Respir Crit Care Med 189;2014:A3988. [Poster abstract]
    Species: Mouse
    Sample type: BAL Fluid
  5. Takahashi, Kazue, Patience Moyo, and Lorencia Chigweshe. "Efficacy of Recombinant Chimeric Lectins, Consisting of Mannose Binding Lectin and L-ficolin, against Influenza A Viral Infection in Mouse Model Study." Virus Research. ScienceDirect, 26 Dec. 2013. Web.
    Species: Mouse
    Sample type: Tissue Lysate
  6. Owens, Philip, et al. "Bone morphogenetic proteins stimulate mammary fibroblasts to promote mammary carcinoma cell invasion."?PloS one?8.6 (2013): e67533.
    Species: Mouse
    Sample type: Conditioned Media
  7. El Behi, Mohamed, et al. "An essential role for decorin in bladder cancer invasiveness."?EMBO molecular medicine?5.12 (2013): 1835-1851.
    Species: Mouse
    Sample type: Conditioned Media
  8. Bobrie A., Krumeich S., Reyal F., et al. Rab27a supports exosome-dependent and -independent mechanisms that modify the tumor microenvironment and can promote tumor progression. Cancer Res. 2012 Oct 1;72(19):4920-30. doi: 10.1158/0008-5472.CAN-12-0925
    Species: Mouse
    Sample type: Conditioned Media
  9. Owens P, Pickup MW, Novitskiy SV, et al. Disruption of bone morphogenetic protein receptor 2 (BMPR2) in mammary tumors promotes metastases through cell autonomous and paracrine mediators. Proceedings of the National Academy of Sciences of the United States of America 2012;109(8):2814-2819. doi:10.1073/pnas.1101139108.
    Species: Mouse
    Sample type: Tissue Lysate
  10. Ge, Qian, et al. "Adipokines identified as new downstream targets for adiponectin: lessons from adiponectin-overexpressing or-deficient mice."?American Journal of Physiology-Endocrinology and Metabolism?301.2 (2011): E326-E335.
    Species: Mouse
    Sample type: Conditioned Media
Sensitivity of this array is good. however, the membrane was labelled manually (by pen) and faced membrane to membrane signal variation. Some membranes has to washed more to get clear signal than others.

National Cancer Institute (NCI)
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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