Mouse Inflammation Array C1

RayBio® C-Series Mouse Inflammation Antibody Array 1 Kit. Detects 40 Mouse Inflammatory Factors. Suitable for all liquid sample types.

AAM-INF-1-2
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Antigen Information

Gene Symbols:
CCL1 | CCL11 | CCL2 | CCL24 | CCL25 | CCL3 | CCL5 | CCL9 | CSF1 | CSF2   View more

Assay Format

Number of Targets Detected:
40
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
4 or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
BLC
(CXCL13)
CD30 Ligand
(TNFSF8)
Eotaxin-1
(CCL11)
Eotaxin-2
(MPIF-2/CCL24)
Fas Ligand
(TNFSF6)
Fractalkine
(CX3CL1)
GCSF
GM-CSF
IFN-gamma
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-2
IL-3
IL-4
IL-6
IL-9
IL-10
IL-12 p40/p70
IL-12 p70
  • Gene Symbol: IL12A
  • Accession #: P43431
IL-13
IL-17A
  • Gene Symbol: IL17A
  • Accession #: Q60971
I-TAC
(CXCL11)
KC
(CXCL1)
Leptin
LIX
Lymphotactin
(XCL1)
MCP-1
(CCL2)
M-CSF
MIG
(CXCL9)
MIP-1 alpha
(CCL3)
MIP-1 gamma
RANTES
(CCL5)
SDF-1 alpha
(CXCL12 alpha)
I-309
(TCA-3/CCL1)
TECK
(CCL25)
TIMP-1
TIMP-2
TNF alpha
TNF RI
(TNFRSF1A)
TNF RII
(TNFRSF1B)

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Mouse Inflammation Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Rojewska E., Zychowska M., Piotrowska A., et al. Involvement of Macrophage Inflammatory Protein-1 Family Members in the Development of Diabetic Neuropathy and Their Contribution to Effectiveness of Morphine. Front Immunol. 2018 Mar 12;9:494. doi: 10.3389/fimmu.2018.00494.
    Species: Mouse
    Sample type: Tissue Lysate
  2. Liu H., Yeung O., Li C., et al. NLRP3 Inflammasome Induced Liver Graft Injury through Activation of Telomere-independent RAP1/KC Axis. J Pathol. 2017 Apr 5. doi: 10.1002/path.4901.
    Species: Mouse
    Sample type: Tissue Lysate
  3. Jang E., Nguyen Q., Kim S., et al. Lung-Infiltrating Foxp3+ Regulatory T Cells Are Quantitatively and Qualitatively Different during Eosinophilic and Neutrophilic Allergic Airway Inflammation but Essential To Control the Inflammation. J Immunol. 2017 Dec 15;199(12):3943-3951. doi: 10.4049/jimmunol.1700211
    Species: Mouse
    Sample type: Tissue Lysate
  4. Kuo T., Weng C., Kuo K., et al. APC haploinsufficiency coupled with p53 loss sufficiently induces mucinous cystic neoplasms and invasive pancreatic carcinoma in mice. Oncogene. 2016 Apr 28;35(17):2223-34. doi: 10.1038/onc.2015.284.
    Species: Mouse
    Sample type: Conditioned Media
  5. Lin J., Peng Y., Wang S., et al. Sympathetic Nervous System Control of Carbon Tetrachloride-Induced Oxidative Stress in Liver through ?-Adrenergic Signaling. Oxid Med Cell Longev. 2016;2016:3190617. doi: 10.1155/2016/3190617
    Species: Mouse
    Sample type: Tissue Lysate
  6. Martin C., Ohayon D., Alkan M., et al. Neutrophil-Expressed p21/waf1 Favors Inflammation Resolution in Pseudomonas aeruginosa Infection. Am J Respir Cell Mol Biol. 2016 May;54(5):740-50. doi: 10.1165/rcmb.2015-0047OC.
    Species: Mouse
    Sample type: BAL Fluid
  7. Wen J., Xiao Y., Wang J., et al. Low doses of CMV induce autoimmune-mediated and inflammatory responses in bile duct epithelia of regulatory T cell-depleted neonatal mice. Lab Invest. 2015 Feb;95(2):180-92. doi: 10.1038/labinvest.2014.148
    Species: Mouse
    Sample type: Tissue Lysate
  8. Zychowska M. Rojewska E., Pilat D., Mika J. The Role of Some Chemokines from the CXC Subfamily in a Mouse Model of Diabetic Neuropathy. J Diabetes Res. 2015;2015:750182. doi: 10.1155/2015/750182.
    Species: Mouse
    Sample type: Tissue Lysate
  9. Li X., Zongfang L., Yan J., Tso P. ApoA-IV Regulates Cytokine Responsiveness in LPS-challenged Macrophage Cells. April 2015 The FASEB Journal vol. 29 no. 1 Supplement LB197 .
    Species: Mouse
    Sample type: Conditioned Media
  10. Cai Y., Chen Z., Liu H., et al. Green tea epigallocatechin-3-gallate alleviates Porphyromonas gingivalis-induced periodontitis in mice. Int Immunopharmacol. 2015 Dec;29(2):839-45. doi: 10.1016/j.intimp.2015.08.033.
    Species: Mouse
    Sample type: Serum
This kit costs less than others and covers most of inflammatory cytokines we need in the mouse model.

Purdue University
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.