Mouse Inflammation Array C1

RayBio® C-Series Mouse Inflammation Antibody Array 1 Kit. Detects 40 Mouse Inflammatory Factors. Suitable for all liquid sample types.

$358.55  
AAM-INF-1-2
+

Antigen Information

Gene Symbols:
CCL1 | CCL11 | CCL2 | CCL24 | CCL25 | CCL3 | CCL5 | CCL9 | CSF1 | CSF2   View more

Assay Format

Number of Targets Detected:
40
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
4 or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
BLC
(CXCL13)
CD30 Ligand
(TNFSF8)
Eotaxin-1
(CCL11)
Eotaxin-2
(MPIF-2/CCL24)
Fas Ligand
(TNFSF6)
Fractalkine
(CX3CL1)
GCSF
GM-CSF
IFN-gamma
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-2
IL-3
IL-4
IL-6
IL-9
IL-10
IL-12 p40/p70
IL-12 p70
  • Gene Symbol: IL12A
  • Accession #: P43431
IL-13
IL-17A
  • Gene Symbol: IL17A
  • Accession #: Q60971
I-TAC
(CXCL11)
KC
(CXCL1)
Leptin
LIX
Lymphotactin
(XCL1)
MCP-1
(CCL2)
M-CSF
MIG
(CXCL9)
MIP-1 alpha
(CCL3)
MIP-1 gamma
RANTES
(CCL5)
SDF-1 alpha
(CXCL12 alpha)
I-309
(TCA-3/CCL1)
TECK
(CCL25)
TIMP-1
TIMP-2
TNF alpha
TNF RI
(TNFRSF1A)
TNF RII
(TNFRSF1B)

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Mouse Inflammation Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Yang G., Lee K., Lee M., et al. Inhibition of lipopolysaccharide-induced nitric oxide and prostaglandin E2 production by chloroform fraction of Cudrania tricuspidata in RAW 264.7 macrophages. BMC Complement Altern Med. 2012 Dec 10;12:250. doi: 10.1186/1472-6882-12-250.
    Species: Mouse
    Sample type: Conditioned Media
  2. Zhu J., Su L, Ye L., et al. Thymosin beta 4 ameliorates hyperglycemia and improves insulin resistance of KK Cg-Ay/J mouse. Diabetes Res Clin Pract. 2012 Apr;96(1):53-9. doi: 10.1016/j.diabres.2011.12.009
    Species: Mouse
    Sample type: Plasma
  3. Salerno D., Tront J., Hoffman B., Liebermann D., et al. Gadd45a and Gadd45b modulate innate immune functions of granulocytes and macrophages by differential regulation of p38 and JNK signaling. J Cell Physiol. 2012 Nov;227(11):3613-20. doi: 10.1002/jcp.24067.
    Species: Mouse
    Sample type: Conditioned Media
  4. Ishizaki S., Kasuya Y., Kuroda F., et al. Role of CD69 in acute lung injury. Life Sci. 2012 May 15;90(17-18):657-65. doi: 10.1016/j.lfs.2012.03.018.
    Species: Mouse
    Sample type: BAL Fluid
  5. Puig K., Swigost A., Zhou X., et al. Amyloid Precursor Protein Expression Modulates Intestine Immune Phenotype. J Neuroimmune Pharmacol. 2012 Mar; 7(1): 215-230. doi: 10.1007/s11481-011-9327-y
    Species: Mouse
    Sample type: Tissue Lysate
  6. Bao F., Wu P., Xiao N., et al. Nitric oxide-driven hypoxia initiates synovial angiogenesis, hyperplasia and inflammatory lesions in mice. PLoS One. 2012;7(3):e34494. doi: 10.1371/journal.pone.0034494
    Species: Mouse
    Sample type: Cell Lysate
  7. Roy Chowdhury R, Aachoui Y, Ghosh SK (2012) Effects of Small Intestinal Submucosa (SIS) on the Murine Innate Immune Microenvironment Induced by Heat-Killed Staphylococcus aureus. PLoS ONE 7(11): e48724. doi:10.1371/journal.pone.0048724
    Species: Mouse
    Sample type: Peritoneal Fluid
  8. Shanmugam A, Rajoria S, George LA, Mittelman A, Suriano R, et al. (2012) Synthetic Toll Like Receptor-4 (TLR-4) Agonist Peptides as a Novel Class of Adjuvants. PLoS ONE 7(2): e30839. doi:10.1371/journal.pone.0030839
    Species: Mouse
    Sample type: Conditioned Media
  9. Wang H, Wu Y, Ojcius DM, Yang XF, Zhang C, et al. (2012) Leptospiral Hemolysins Induce Proinflammatory Cytokines through Toll-Like Receptor 2-and 4-Mediated JNK and NF-kB Signaling Pathways. PLoS ONE 7(8): e42266. doi:10.1371/journal.pone.0042266
    Species: Mouse
    Sample type: Serum
  10. Tsuyusaki J., et al. Cigarette smoke-induced pulmonary inflammation is attenuated in CD69-deficient mice. Journal of Receptors and Signal Transduction, 2011; 31(6): 434-439; DOI: 10.3109/10799893.2011.631929
    Species: Mouse
    Sample type: BAL Fluid
The product is amazing. Following the manual, and make sure you wash enough (more buffer or more times) to make your array looking better.

UPMC
My first time using the protein array kit and I am very satisfied with the ease of the whole procedure as well as the results. I ordered 4 more membranes that I am satisfied with.

UCI
Various fluids can be measured by this kit. It is important to select the protein concentration as a positive sample before the experiment. I recommend using a high sensitivity ECL solution for a clear image under the low background.

Yale University
This kit costs less than others and covers most of inflammatory cytokines we need in the mouse model.

Purdue University
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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