Mouse Inflammation Array C1

RayBio® C-Series Mouse Inflammation Antibody Array 1 Kit. Detects 40 Mouse Inflammatory Factors. Suitable for all liquid sample types.

$358.55  
AAM-INF-1-2
+

Antigen Information

Gene Symbols:
CCL1 | CCL11 | CCL2 | CCL24 | CCL25 | CCL3 | CCL5 | CCL9 | CSF1 | CSF2   View more

Assay Format

Number of Targets Detected:
40
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
4 or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
BLC
(CXCL13)
CD30 Ligand
(TNFSF8)
Eotaxin-1
(CCL11)
Eotaxin-2
(MPIF-2/CCL24)
Fas Ligand
(TNFSF6)
Fractalkine
(CX3CL1)
GCSF
GM-CSF
IFN-gamma
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-2
IL-3
IL-4
IL-6
IL-9
IL-10
IL-12 p40/p70
IL-12 p70
  • Gene Symbol: IL12A
  • Accession #: P43431
IL-13
IL-17A
  • Gene Symbol: IL17A
  • Accession #: Q60971
I-TAC
(CXCL11)
KC
(CXCL1)
Leptin
LIX
Lymphotactin
(XCL1)
MCP-1
(CCL2)
M-CSF
MIG
(CXCL9)
MIP-1 alpha
(CCL3)
MIP-1 gamma
RANTES
(CCL5)
SDF-1 alpha
(CXCL12 alpha)
I-309
(TCA-3/CCL1)
TECK
(CCL25)
TIMP-1
TIMP-2
TNF alpha
TNF RI
(TNFRSF1A)
TNF RII
(TNFRSF1B)

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Mouse Inflammation Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Fang TC., Pang CY., Chiu SC., et al. Renoprotective effect of human umbilical cord-derived mesenchymal stem cells in immunodeficient mice suffering from acute kidney injury. PLoS One. 2012;7(9):e46504. doi: 10.1371/journal.pone.0046504.
    Species: Mouse
    Sample type: Tissue Lysate
  2. Lalancette-Hébert M., Swarup V., Beaulieu JM., et al. Galectin-3 is required for resident microglia activation and proliferation in response to ischemic injury. J Neurosci. 2012 Jul 25;32(30):10383-95. doi: 10.1523/JNEUROSCI.1498-12.2012
    Species: Mouse
    Sample type: Tissue Lysate
  3. Maalouf SW, Theivakumar S, Owens DM. Epidermal ?6?4 integrin stimulates the influx of immunosuppressive cells during skin tumor promotion. Journal of Dermatological Science. 2012;66(2):108-118. doi:10.1016/j.jdermsci.2012.02.009.
    Species: Mouse
    Sample type: Tissue Lysate
  4. Slesiona S., Ibrahim-Granet O., Olias P., et al. Murine infection models for Aspergillus terreus pulmonary aspergillosis reveal long-term persistence of conidia and liver degeneration. J Infect Dis. 2012 Apr 15;205(8):1268-77. doi: 10.1093/infdis/jis193.
    Species: Mouse
    Sample type: Tissue Lysate
  5. Chien MH., Bien MY., Ku CC., et al. Systemic human orbital fat-derived stem/stromal cell transplantation ameliorates acute inflammation in lipopolysaccharide-induced acute lung injury. Crit Care Med. 2012 Apr;40(4):1245-53. doi: 10.1097/CCM.0b013e31823bc89a.
    Species: Mouse
    Sample type: Serum
  6. Tang A., Li N., Li X., et al. Dynamic activation of the key pathways: linking colitis to colorectal cancer in a mouse model. Carcinogenesis. 2012 Jul;33(7):1375-83. doi: 10.1093/carcin/bgs183
    Species: Mouse
    Sample type: Serum
  7. Yang G., Lee K., Lee M., et al. Inhibition of lipopolysaccharide-induced nitric oxide and prostaglandin E2 production by chloroform fraction of Cudrania tricuspidata in RAW 264.7 macrophages. BMC Complement Altern Med. 2012 Dec 10;12:250. doi: 10.1186/1472-6882-12-250.
    Species: Mouse
    Sample type: Conditioned Media
  8. Zhu J., Su L, Ye L., et al. Thymosin beta 4 ameliorates hyperglycemia and improves insulin resistance of KK Cg-Ay/J mouse. Diabetes Res Clin Pract. 2012 Apr;96(1):53-9. doi: 10.1016/j.diabres.2011.12.009
    Species: Mouse
    Sample type: Plasma
  9. Salerno D., Tront J., Hoffman B., Liebermann D., et al. Gadd45a and Gadd45b modulate innate immune functions of granulocytes and macrophages by differential regulation of p38 and JNK signaling. J Cell Physiol. 2012 Nov;227(11):3613-20. doi: 10.1002/jcp.24067.
    Species: Mouse
    Sample type: Conditioned Media
  10. Ishizaki S., Kasuya Y., Kuroda F., et al. Role of CD69 in acute lung injury. Life Sci. 2012 May 15;90(17-18):657-65. doi: 10.1016/j.lfs.2012.03.018.
    Species: Mouse
    Sample type: BAL Fluid
The product is amazing. Following the manual, and make sure you wash enough (more buffer or more times) to make your array looking better.

UPMC
My first time using the protein array kit and I am very satisfied with the ease of the whole procedure as well as the results. I ordered 4 more membranes that I am satisfied with.

UCI
Various fluids can be measured by this kit. It is important to select the protein concentration as a positive sample before the experiment. I recommend using a high sensitivity ECL solution for a clear image under the low background.

Yale University
This kit costs less than others and covers most of inflammatory cytokines we need in the mouse model.

Purdue University
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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