Mouse Inflammation Array C1

RayBio® C-Series Mouse Inflammation Antibody Array 1 Kit. Detects 40 Mouse Inflammatory Factors. Suitable for all liquid sample types.


Assay Format

Number of Targets Detected:
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
  • Semi-Quantitative
Solid Support:

Product Specifications

4 or 8 Sample Kit

Protein Information

Gene Symbols:
CCL1 | CCL11 | CCL2 | CCL24 | CCL25 | CCL3 | CCL5 | CCL9 | CSF1 | CSF2   View more

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
CD30 Ligand
Fas Ligand
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-12 p40/p70
IL-12 p70
  • Gene Symbol: IL12A
  • Accession #: P43431
  • Gene Symbol: IL17A
  • Accession #: Q60971
MIP-1 alpha
MIP-1 gamma
SDF-1 alpha
(CXCL12 alpha)
TNF alpha

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Mouse Inflammation Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis


For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Adkins I, Kamanova J, Kocourkova A, Svedova M, Tomala J, et al. (2014) Bordetella Adenylate Cyclase Toxin Differentially Modulates Toll-Like Receptor-Stimulated Activation, Migration and T Cell Stimulatory Capacity of Dendritic Cells. PLoS ONE 9(8): e104064. doi:10.1371/journal.pone.0104064
    Species: Mouse
    Sample type: Conditioned Media (BMDC cultures)
  2. Adkins I., et al. Bordetella Adenylate Cyclase Toxin Differentially Modulates Toll-Like Receptor-Stimulated Activation, Migration and T Cell Stimulatory Capacity of Dendritic Cells. PLOS One 2014. Published: August 01, 2014. DOI: 10.1371/journal.pone.0104064
    Species: Mouse
    Sample type: Conditioned Media (BMDC cell culture sup)
  3. Bao F., Wu P., Xiao N., et al. Nitric oxide-driven hypoxia initiates synovial angiogenesis, hyperplasia and inflammatory lesions in mice. PLoS One. 2012;7(3):e34494. doi: 10.1371/journal.pone.0034494
    Species: Mouse
    Sample type: Cell Lysate (Blood lysate samples)
  4. Roy Chowdhury R, Aachoui Y, Ghosh SK (2012) Effects of Small Intestinal Submucosa (SIS) on the Murine Innate Immune Microenvironment Induced by Heat-Killed Staphylococcus aureus. PLoS ONE 7(11): e48724. doi:10.1371/journal.pone.0048724
    Species: Mouse
    Sample type: Peritoneal Fluid (Staph Aureus induced inflammation, Balb C mice)
  5. Shanmugam A, Rajoria S, George LA, Mittelman A, Suriano R, et al. (2012) Synthetic Toll Like Receptor-4 (TLR-4) Agonist Peptides as a Novel Class of Adjuvants. PLoS ONE 7(2): e30839. doi:10.1371/journal.pone.0030839
    Species: Mouse
    Sample type: Conditioned Media (Raw264.7 cells stimulated with LPS)
  6. Wang H, Wu Y, Ojcius DM, Yang XF, Zhang C, et al. (2012) Leptospiral Hemolysins Induce Proinflammatory Cytokines through Toll-Like Receptor 2-and 4-Mediated JNK and NF-kB Signaling Pathways. PLoS ONE 7(8): e42266. doi:10.1371/journal.pone.0042266
    Species: Mouse
    Sample type: Serum (C3H-HeJ mice injected ip with EMJH media (culture media) or leptospirosis infection)
  7. Aachoui, Youssef, and Swapan K. Ghosh. "Extracellular matrix from porcine small intestinal submucosa (SIS) as immune adjuvants." PloS one 6.11 (2011): e27083.
    Species: Mouse
    Sample type: Conditioned Media (peritoneal exudate cells (PECs) from BALB/c mice were injected with adjuvants)
  8. Jrad-Lamine, Aicha, et al. "Indoleamine 2, 3-dioxygenase 1 (IDO1) is involved in the control of mouse caput epididymis immune environment." PloS one 8.6 (2013): e66494.
    Species: Mouse
    Sample type: Plasma (caput epididymal protein extracts )
  9. Jrad-Lamine, Aicha, et al. "Indoleamine 2, 3-dioxygenase 1 (IDO1) is involved in the control of mouse caput epididymis immune environment." PloS one 8.6 (2013): e66494.
    Species: Mouse
    Sample type: Tissue Lysate (caput epididymal protein extracts )
  10. Aachoui, Youssef, and Swapan K. Ghosh. "Immune enhancement by novel vaccine adjuvants in autoimmune-prone NZB/W F1 mice: relative efficacy and safety." BMC immunology 12.1 (2011): 61.
    Species: Mouse
    Sample type: Serum (mice treated with different adjuvants in combination with antigen after receiving two booster immunizations)
It is a quick way to observe the inflammatory cytokine and chemokine profile of mouse tumors. Multiple washes help reduce background noise.

Ohio State University
It's a quick inflammation array and really helpful. Hope to have more further discount in the future order.

SUNY Downstate Health Sciences University
The product is amazing. Following the manual, and make sure you wash enough (more buffer or more times) to make your array looking better.

My first time using the protein array kit and I am very satisfied with the ease of the whole procedure as well as the results. I ordered 4 more membranes that I am satisfied with.

Various fluids can be measured by this kit. It is important to select the protein concentration as a positive sample before the experiment. I recommend using a high sensitivity ECL solution for a clear image under the low background.

Yale University
This kit costs less than others and covers most of inflammatory cytokines we need in the mouse model.

Purdue University
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This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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