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Home/Search results/Products/Arrays/Antibody Arrays/C-Series/Inflammation Arrays/Mouse Inflammation Array C1

Manual / SDS

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Assay Format

Number of Targets Detected:

Species Detected:

Mouse

Compatible Sample Types:

Cell Culture Supernatants
Cell Lysates
Plasma
Serum
Tissue Lysates

Design Principle:

Sandwich-based

Method of Detection:

Chemiluminescence

Quantitative/Semi-Quantitative:

Semi-Quantitative

Solid Support:

Membrane

Product Specifications

Size:

4 or 8 Sample Kit

Protein Information

Gene Symbols:

CCL1 | CCL11 | CCL2 | CCL24 | CCL25 | CCL3 | CCL5 | CCL9 | CSF1 | CSF2 View more

Product Features

Easy to use
No specialized equipment needed
Compatible with nearly any liquid sample
Proven technology (many publications)
Highly sensitive (pg/ml)
Sandwich ELISA specificity
Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
BLC (CXCL13) Gene Symbol: CXCL13 Accession #: O55038 ELISA Kit: RayBio Mouse BLC ELISA	CD30 Ligand (TNFSF8) Gene Symbol: CD30LG Accession #: P32972 ELISA Kit: RayBio Mouse CD30 L ELISA	Eotaxin-1 (CCL11) Gene Symbol: CCL11 Accession #: P48298 ELISA Kit: RayBio Mouse Eotaxin-1 ELISA	Eotaxin-2 (MPIF-2/CCL24) Gene Symbol: Ccl24 Accession #: Q9JKC0 ELISA Kit: RayBio Mouse Eotaxin-2 ELISA	Fas Ligand (TNFSF6) Gene Symbol: FASLG Accession #: P41047 ELISA Kit: RayBio Mouse Fas L ELISA
Fractalkine (CX3CL1) Gene Symbol: CX3CL1 Accession #: O35188 ELISA Kit: RayBio Mouse Fractalkine ELISA	GCSF Gene Symbol: CSF3 Accession #: P09920 ELISA Kit: RayBio Mouse G-CSF ELISA	GM-CSF Gene Symbol: CSF2 Accession #: P26955 ELISA Kit: RayBio Mouse GM-CSF ELISA	IFN-gamma Gene Symbol: IFNG Accession #: P01580 ELISA Kit: RayBio Mouse IFN-gamma ELISA	IL-1 alpha (IL-1 F1) Gene Symbol: IL1A Accession #: P01582 ELISA Kit: RayBio Mouse IL-1 alpha ELISA
IL-1 beta (IL-1 F2) Gene Symbol: IL1B Accession #: P10749 ELISA Kit: RayBio Mouse IL-1 beta ELISA	IL-2 Gene Symbol: IL2 Accession #: Q791T3 ELISA Kit: RayBio Mouse IL-2 ELISA	IL-3 Gene Symbol: IL3 Accession #: P01586 ELISA Kit: RayBio Mouse IL-3 ELISA	IL-4 Gene Symbol: IL4 Accession #: P07750 ELISA Kit: RayBio Mouse IL-4 ELISA	IL-6 Gene Symbol: IL6 Accession #: P08505 ELISA Kit: RayBio Mouse IL-6 ELISA
IL-9 Gene Symbol: IL9 Accession #: P15247 ELISA Kit: RayBio Mouse IL-9 ELISA	IL-10 Gene Symbol: IL10 Accession #: Q0VBJ1 ELISA Kit: RayBio Mouse IL-10 ELISA	IL-12 p40/p70 Gene Symbol: IL12A; IL12B Accession #: P43432 ELISA Kit: RayBio Mouse IL-12 p40/70 ELISA	IL-12 p70 Gene Symbol: IL12A Accession #: P43431	IL-13 Gene Symbol: IL13 Accession #: P20109 ELISA Kit: RayBio Mouse IL-13 ELISA
IL-17A Gene Symbol: IL17A Accession #: Q60971	I-TAC (CXCL11) Gene Symbol: CXCL11 Accession #: Q9JHH5 ELISA Kit: RayBio Mouse I-TAC ELISA	KC (CXCL1) Gene Symbol: CXCL1 Accession #: P12850 ELISA Kit: RayBio Mouse KC ELISA	Leptin Gene Symbol: LEP Accession #: P41160 ELISA Kit: RayBio Mouse Leptin ELISA	LIX Gene Symbol: Cxcl5 Accession #: P50228 ELISA Kit: RayBio Mouse LIX ELISA
Lymphotactin (XCL1) Gene Symbol: XCL1 Accession #: P47993 ELISA Kit: RayBio Mouse Lymphotactin ELISA	MCP-1 (CCL2) Gene Symbol: CCL2 Accession #: Q9QYD7 ELISA Kit: RayBio Mouse MCP-1 ELISA	M-CSF Gene Symbol: CSF1 Accession #: P07141 ELISA Kit: RayBio Mouse M-CSF ELISA	MIG (CXCL9) Gene Symbol: CXCL9 Accession #: P18340 ELISA Kit: RayBio Mouse MIG ELISA	MIP-1 alpha (CCL3) Gene Symbol: CCL3 Accession #: P14096 ELISA Kit: RayBio Mouse MIP-1 alpha ELISA
MIP-1 gamma Gene Symbol: Ccl9 Accession #: Q5QNW2 ELISA Kit: RayBio Mouse MIP-1 gamma ELISA	RANTES (CCL5) Gene Symbol: CCL5 Accession #: P30882 ELISA Kit: RayBio Mouse RANTES ELISA	SDF-1 alpha (CXCL12 alpha) Gene Symbol: CXCL12 Accession #: P40224 ELISA Kit: RayBio Mouse SDF-1 alpha ELISA	I-309 (TCA-3/CCL1) Gene Symbol: CCL1 Accession #: P14098 ELISA Kit: RayBio Mouse TCA-3 ELISA	TECK (CCL25) Gene Symbol: CCL25 Accession #: O35903 ELISA Kit: RayBio Mouse TECK ELISA
TIMP-1 Gene Symbol: TIMP1 Accession #: P12032 ELISA Kit: RayBio Mouse TIMP-1 ELISA	TIMP-2 Gene Symbol: TIMP2 Accession #: Q6GXA8 ELISA Kit: RayBio Mouse TIMP-2 ELISA	TNF alpha Gene Symbol: TNF Accession #: Q62326 ELISA Kit: RayBio Mouse TNF-alpha ELISA	TNF RI (TNFRSF1A) Gene Symbol: TNFRSF1A Accession #: P25118 ELISA Kit: RayBio Mouse sTNFRI ELISA	TNF RII (TNFRSF1B) Gene Symbol: TNFRSF1B Accession #: O88734 ELISA Kit: RayBio Mouse sTNFRII ELISA

Application Notes

Suggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process

Kit Components

Mouse Inflammation Antibody Array C1 Membranes
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated HRP
Detection Buffer C
Detection Buffer D
Lysis Buffer
8-Well Incubation Tray
Plastic Sheets
Array Templates
Manual

Other Materials Required

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It^® or EpiChem II Benchtop Darkroom or GE's ImageQuant^™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Protocol Outline

Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Pinto, B., Morelli, G., Rastogi, M., Savardi, A., Fumagalli, A., Petretto, A., Cancedda, L. (2020). Rescuing Over-activated Microglia Restores Cognitive Performance in Juvenile Animals of the Dp(16) Mouse Model of Down Syndrome. Neuron.
Species: Mouse
Sample type: Tissue Lysate
Rojewska E., Zychowska M., Piotrowska A., et al. Involvement of Macrophage Inflammatory Protein-1 Family Members in the Development of Diabetic Neuropathy and Their Contribution to Effectiveness of Morphine. Front Immunol. 2018 Mar 12;9:494. doi: 10.3389/fimmu.2018.00494.
Species: Mouse
Sample type: Tissue Lysate
Liu H., Yeung O., Li C., et al. NLRP3 Inflammasome Induced Liver Graft Injury through Activation of Telomere-independent RAP1/KC Axis. J Pathol. 2017 Apr 5. doi: 10.1002/path.4901.
Species: Mouse
Sample type: Tissue Lysate
Jang E., Nguyen Q., Kim S., et al. Lung-Infiltrating Foxp3+ Regulatory T Cells Are Quantitatively and Qualitatively Different during Eosinophilic and Neutrophilic Allergic Airway Inflammation but Essential To Control the Inflammation. J Immunol. 2017 Dec 15;199(12):3943-3951. doi: 10.4049/jimmunol.1700211
Species: Mouse
Sample type: Tissue Lysate
Venkatesh A, Nandigam H, Muccioli M, Singh M, Loftus T, Lewis D, Pate M, Benencia F. Regulation of inflammatory factors by double-stranded RNA receptors in breast cancer cells. Immunobiology. 2018 Jun-Jul;223(6-7):466-476. doi: 10.1016/j.imbio.2017.11.005. Epub 2017 Nov 22. PMID: 29331323.
Species: Mouse
Sample type: Conditioned Media
Kuo T., Weng C., Kuo K., et al. APC haploinsufficiency coupled with p53 loss sufficiently induces mucinous cystic neoplasms and invasive pancreatic carcinoma in mice. Oncogene. 2016 Apr 28;35(17):2223-34. doi: 10.1038/onc.2015.284.
Species: Mouse
Sample type: Conditioned Media
Lin J., Peng Y., Wang S., et al. Sympathetic Nervous System Control of Carbon Tetrachloride-Induced Oxidative Stress in Liver through ?-Adrenergic Signaling. Oxid Med Cell Longev. 2016;2016:3190617. doi: 10.1155/2016/3190617
Species: Mouse
Sample type: Tissue Lysate
Martin C., Ohayon D., Alkan M., et al. Neutrophil-Expressed p21/waf1 Favors Inflammation Resolution in Pseudomonas aeruginosa Infection. Am J Respir Cell Mol Biol. 2016 May;54(5):740-50. doi: 10.1165/rcmb.2015-0047OC.
Species: Mouse
Sample type: BAL Fluid
Wen J., Xiao Y., Wang J., et al. Low doses of CMV induce autoimmune-mediated and inflammatory responses in bile duct epithelia of regulatory T cell-depleted neonatal mice. Lab Invest. 2015 Feb;95(2):180-92. doi: 10.1038/labinvest.2014.148
Species: Mouse
Sample type: Tissue Lysate
Zychowska M. Rojewska E., Pilat D., Mika J. The Role of Some Chemokines from the CXC Subfamily in a Mouse Model of Diabetic Neuropathy. J Diabetes Res. 2015;2015:750182. doi: 10.1155/2015/750182.
Species: Mouse
Sample type: Tissue Lysate

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Best way to evaluate quickly imflammatory cytokines in different tissues. Very easy to use. I advise different washes and revelation of all the membranes at the same time for 2 min between the 2 given plastic sheets. Would be even better to includes other cytokines like TGFb in the panel

Matthias Dedobbeleer
Sickkids Hospital 02/27/2023, 02:38 PM

It is a quick way to observe the inflammatory cytokine and chemokine profile of mouse tumors. Multiple washes help reduce background noise.

Anonymous
Ohio State University 07/21/2022, 04:47 PM

It's a quick inflammation array and really helpful. Hope to have more further discount in the future order.

Robert Chiang
SUNY Downstate Health Sciences University 01/26/2021, 09:05 PM

The product is amazing. Following the manual, and make sure you wash enough (more buffer or more times) to make your array looking better.

Nico
UPMC 05/12/2020, 04:39 PM

My first time using the protein array kit and I am very satisfied with the ease of the whole procedure as well as the results. I ordered 4 more membranes that I am satisfied with.

Aicha Saadane
UCI 12/16/2019, 04:35 PM

Various fluids can be measured by this kit. It is important to select the protein concentration as a positive sample before the experiment. I recommend using a high sensitivity ECL solution for a clear image under the low background.

Hyuk-Kwon Kwon
Yale University 06/28/2019, 05:11 PM

This kit costs less than others and covers most of inflammatory cytokines we need in the mouse model.

Christina
Purdue University 05/23/2018, 05:25 PM

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