Mouse Inflammation Array C1

RayBio® C-Series Mouse Inflammation Antibody Array 1 Kit. Detects 40 Mouse Inflammatory Factors. Suitable for all liquid sample types.

$358.55  
AAM-INF-1-2
+

Antigen Information

Gene Symbols:
CCL1 | CCL11 | CCL2 | CCL24 | CCL25 | CCL3 | CCL5 | CCL9 | CSF1 | CSF2   View more

Assay Format

Number of Targets Detected:
40
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
4 or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
BLC
(CXCL13)
CD30 Ligand
(TNFSF8)
Eotaxin-1
(CCL11)
Eotaxin-2
(MPIF-2/CCL24)
Fas Ligand
(TNFSF6)
Fractalkine
(CX3CL1)
GCSF
GM-CSF
IFN-gamma
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-2
IL-3
IL-4
IL-6
IL-9
IL-10
IL-12 p40/p70
IL-12 p70
  • Gene Symbol: IL12A
  • Accession #: P43431
IL-13
IL-17A
  • Gene Symbol: IL17A
  • Accession #: Q60971
I-TAC
(CXCL11)
KC
(CXCL1)
Leptin
LIX
Lymphotactin
(XCL1)
MCP-1
(CCL2)
M-CSF
MIG
(CXCL9)
MIP-1 alpha
(CCL3)
MIP-1 gamma
RANTES
(CCL5)
SDF-1 alpha
(CXCL12 alpha)
I-309
(TCA-3/CCL1)
TECK
(CCL25)
TIMP-1
TIMP-2
TNF alpha
TNF RI
(TNFRSF1A)
TNF RII
(TNFRSF1B)

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Mouse Inflammation Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Puig K., Lutz B., Urguhart S., et al. Overexpression of mutant amyloid-? protein precursor and presenilin 1 modulates enteric nervous system. J Alzheimers Dis. 2015;44(4):1263-78. doi: 10.3233/JAD-142259.
    Species: Mouse
    Sample type: Tissue Lysate
  2. Adkins I, Kamanova J, Kocourkova A, Svedova M, Tomala J, et al. (2014) Bordetella Adenylate Cyclase Toxin Differentially Modulates Toll-Like Receptor-Stimulated Activation, Migration and T Cell Stimulatory Capacity of Dendritic Cells. PLoS ONE 9(8): e104064. doi:10.1371/journal.pone.0104064
    Species: Mouse
    Sample type: Conditioned Media
  3. Adkins I., et al. Bordetella Adenylate Cyclase Toxin Differentially Modulates Toll-Like Receptor-Stimulated Activation, Migration and T Cell Stimulatory Capacity of Dendritic Cells. PLOS One 2014. Published: August 01, 2014. DOI: 10.1371/journal.pone.0104064
    Species: Mouse
    Sample type: Conditioned Media
  4. Ke, F., Zhang, L. et al. Autocrine IL-6 drives skin-derived mesenchymal stem cell trafficking via regulating voltage-gated Ca2+ channels. Stem Cells 2014. Epub Ahead of Print. DOI: 10.1002/stem.1763
    Species: Mouse
    Sample type: Conditioned Media
  5. Patel P., Julien JP., Kriz J. Early-Stage Treatment with Withaferin A Reduces Levels of Misfolded Superoxide Dismutase 1 and Extends Lifespan in a Mouse Model of Amyotrophic Lateral Sclerosis. Neurotherapeutics. 2014 Nov 18. [Epub ahead of print]
    Species: Mouse
    Sample type: Tissue Lysate
  6. Soki F., Polarization of Prostate Cancer Associated Macrophages is Induced by Milk-Fat Globule-EGF Factor 8 (MFG-E8) Mediated Efferocytosis. JBC Papers in Press. Published on July 8, 2014. http://www.jbc.org/cgi/doi/10.1074/jbc.M114.571620
    Species: Mouse
    Sample type: Conditioned Media
  7. Sun H., Adjuvant-active fraction from Albizia julibrissin saponins improves immune responses by inducing cytokine and chemokine at the site of injection. International Immunopharmacology 22 (2014) 346-355. http://dx.doi.org/10.1016/j.intimp.2014.07.021
    Species: Mouse
    Sample type: Tissue Lysate
  8. Wu Z., Chen X., Liu F., Chen W., Wu P., Wieschhaus A., Chishti A., Roche P., Chen W., Lin TJ. Calpain-1 Contributes to IgE-Mediated Mast Cell Activation. The Journal of Immunology April 23, 2014 1301677. doi: 10.4049/? [Epub ahead of print]
    Species: Mouse
    Sample type: Conditioned Media
  9. Jrad-Lamine, Aicha, et al. "Indoleamine 2, 3-dioxygenase 1 (IDO1) is involved in the control of mouse caput epididymis immune environment."?PloS one?8.6 (2013): e66494.
    Species: Mouse
    Sample type: Tissue Lysate
  10. Zychowska, Magdalena, et al. "Minocycline influences the anti-inflammatory interleukins and enhances the effectiveness of morphine under mice diabetic neuropathy."?Journal of neuroimmunology?262.1 (2013): 35-45.
    Species: Mouse
    Sample type: Tissue Lysate
The product is amazing. Following the manual, and make sure you wash enough (more buffer or more times) to make your array looking better.

UPMC
My first time using the protein array kit and I am very satisfied with the ease of the whole procedure as well as the results. I ordered 4 more membranes that I am satisfied with.

UCI
Various fluids can be measured by this kit. It is important to select the protein concentration as a positive sample before the experiment. I recommend using a high sensitivity ECL solution for a clear image under the low background.

Yale University
This kit costs less than others and covers most of inflammatory cytokines we need in the mouse model.

Purdue University
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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