Mouse Inflammation Array C1

RayBio® C-Series Mouse Inflammation Antibody Array 1 Kit. Detects 40 Mouse Inflammatory Factors. Suitable for all liquid sample types.


Assay Format

Number of Targets Detected:
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
  • Semi-Quantitative
Solid Support:

Product Specifications

4 or 8 Sample Kit

Protein Information

Gene Symbols:
CCL1 | CCL11 | CCL2 | CCL24 | CCL25 | CCL3 | CCL5 | CCL9 | CSF1 | CSF2   View more

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
CD30 Ligand
Fas Ligand
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-12 p40/p70
IL-12 p70
  • Gene Symbol: IL12A
  • Accession #: P43431
  • Gene Symbol: IL17A
  • Accession #: Q60971
MIP-1 alpha
MIP-1 gamma
SDF-1 alpha
(CXCL12 alpha)
TNF alpha

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Mouse Inflammation Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis


For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Pinto, B., Morelli, G., Rastogi, M., Savardi, A., Fumagalli, A., Petretto, A., … Cancedda, L. (2020). Rescuing Over-activated Microglia Restores Cognitive Performance in Juvenile Animals of the Dp(16) Mouse Model of Down Syndrome. Neuron.
    Species: Mouse
    Sample type: Tissue Lysate
  2. Rojewska E., Zychowska M., Piotrowska A., et al. Involvement of Macrophage Inflammatory Protein-1 Family Members in the Development of Diabetic Neuropathy and Their Contribution to Effectiveness of Morphine. Front Immunol. 2018 Mar 12;9:494. doi: 10.3389/fimmu.2018.00494.
    Species: Mouse
    Sample type: Tissue Lysate
  3. Liu H., Yeung O., Li C., et al. NLRP3 Inflammasome Induced Liver Graft Injury through Activation of Telomere-independent RAP1/KC Axis. J Pathol. 2017 Apr 5. doi: 10.1002/path.4901.
    Species: Mouse
    Sample type: Tissue Lysate
  4. Jang E., Nguyen Q., Kim S., et al. Lung-Infiltrating Foxp3+ Regulatory T Cells Are Quantitatively and Qualitatively Different during Eosinophilic and Neutrophilic Allergic Airway Inflammation but Essential To Control the Inflammation. J Immunol. 2017 Dec 15;199(12):3943-3951. doi: 10.4049/jimmunol.1700211
    Species: Mouse
    Sample type: Tissue Lysate
  5. Venkatesh A, Nandigam H, Muccioli M, Singh M, Loftus T, Lewis D, Pate M, Benencia F. Regulation of inflammatory factors by double-stranded RNA receptors in breast cancer cells. Immunobiology. 2018 Jun-Jul;223(6-7):466-476. doi: 10.1016/j.imbio.2017.11.005. Epub 2017 Nov 22. PMID: 29331323.
    Species: Mouse
    Sample type: Conditioned Media
  6. Kuo T., Weng C., Kuo K., et al. APC haploinsufficiency coupled with p53 loss sufficiently induces mucinous cystic neoplasms and invasive pancreatic carcinoma in mice. Oncogene. 2016 Apr 28;35(17):2223-34. doi: 10.1038/onc.2015.284.
    Species: Mouse
    Sample type: Conditioned Media
  7. Lin J., Peng Y., Wang S., et al. Sympathetic Nervous System Control of Carbon Tetrachloride-Induced Oxidative Stress in Liver through ?-Adrenergic Signaling. Oxid Med Cell Longev. 2016;2016:3190617. doi: 10.1155/2016/3190617
    Species: Mouse
    Sample type: Tissue Lysate
  8. Martin C., Ohayon D., Alkan M., et al. Neutrophil-Expressed p21/waf1 Favors Inflammation Resolution in Pseudomonas aeruginosa Infection. Am J Respir Cell Mol Biol. 2016 May;54(5):740-50. doi: 10.1165/rcmb.2015-0047OC.
    Species: Mouse
    Sample type: BAL Fluid
  9. Wen J., Xiao Y., Wang J., et al. Low doses of CMV induce autoimmune-mediated and inflammatory responses in bile duct epithelia of regulatory T cell-depleted neonatal mice. Lab Invest. 2015 Feb;95(2):180-92. doi: 10.1038/labinvest.2014.148
    Species: Mouse
    Sample type: Tissue Lysate
  10. Zychowska M. Rojewska E., Pilat D., Mika J. The Role of Some Chemokines from the CXC Subfamily in a Mouse Model of Diabetic Neuropathy. J Diabetes Res. 2015;2015:750182. doi: 10.1155/2015/750182.
    Species: Mouse
    Sample type: Tissue Lysate
Best way to evaluate quickly imflammatory cytokines in different tissues. Very easy to use. I advise different washes and revelation of all the membranes at the same time for 2 min between the 2 given plastic sheets. Would be even better to includes other cytokines like TGFb in the panel

Sickkids Hospital
It is a quick way to observe the inflammatory cytokine and chemokine profile of mouse tumors. Multiple washes help reduce background noise.

Ohio State University
It's a quick inflammation array and really helpful. Hope to have more further discount in the future order.

SUNY Downstate Health Sciences University
The product is amazing. Following the manual, and make sure you wash enough (more buffer or more times) to make your array looking better.

My first time using the protein array kit and I am very satisfied with the ease of the whole procedure as well as the results. I ordered 4 more membranes that I am satisfied with.

Various fluids can be measured by this kit. It is important to select the protein concentration as a positive sample before the experiment. I recommend using a high sensitivity ECL solution for a clear image under the low background.

Yale University
This kit costs less than others and covers most of inflammatory cytokines we need in the mouse model.

Purdue University
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This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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