Rat Cytokine Array C1

RayBio® C-Series Rat Cytokine Antibody Array 1 Kit. Detects 19 Rat Cytokines. Suitable for all liquid sample types.

$293.91  
AAR-CYT-1-2
+

Antigen Information

Gene Symbols:
CCL2 | CCL20 | CNTF | CSF2 | CX3CL1 | CXCL2 | CXCL3 | CXCL5 | IFNG | IL10   View more

Assay Format

Number of Targets Detected:
19
Species Detected:
  • Rat
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
4 or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
CINC-2
CINC-3
CNTF
Fractalkine
(CX3CL1)
GM-CSF
IFN-gamma
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-4
IL-6
IL-10
LIX
Leptin
MCP-1
(CCL2)
MIP-3 alpha
(CCL20)
beta-NGF
TIMP-1
TNF alpha
VEGF-A

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Rat Cytokine Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Busch-Dienstfertig M., Labuz D., Wolfram T., et al. JAK-STAT1/3-induced expression of signal sequence-encoding proopiomelanocortin mRNA in lymphocytes reduces inflammatory pain in rats. Mol Pain. 2012 Nov 13;8:83. doi: 10.1186/1744-8069-8-83
    Species: Rat
    Sample type: Tissue Lysate
  2. Merabova N, Kaminski R, Krynska B, Amini S, Khalili K, Darbinyan A. JCV agnoprotein-induced reduction in CXCL5/LIX secretion by oligodendrocytes is associated with activation of apoptotic signaling in neurons. Journal of Cellular Physiology. 2012;227(8):3119-3127. doi:10.1002/jcp.23065.
    Species: Rat
    Sample type: Conditioned Media
  3. Schaal SM, Garg MS, Ghosh M, Lovera L, Lopez M, et al. (2012) The Therapeutic Profile of Rolipram, PDE Target and Mechanism of Action as aNeuroprotectant following Spinal Cord Injury. PLoS ONE 7(9): e43634. doi:10.1371/journal.pone.0043634
    Species: Rat
    Sample type: Tissue Lysate
  4. Liu J., Xu C., Chen L., et al. Involvement of Kv1.3 and p38 MAPK signaling in HIV-1 glycoprotein 120-induced microglia neurotoxicity. Cell Death Dis. 2012 Jan 19;3:e254. doi: 10.1038/cddis.2011.140.
    Species: Ray
    Sample type: Conditioned Media
  5. Ogawa T., de Bold AJ. Uncoordinated regulation of atrial natriuretic factor and brain natriuretic peptide in lipopolysaccharide-treated rats. Biomarkers. 2012 Mar;17(2):140-9. doi: 10.3109/1354750X.2011.643487.
    Species: Rat
    Sample type: Plasma
  6. Holubova M., Leva M., Sedmikova M., et al. Characterization of three newly established rat sarcoma cell clones. In Vitro Cell Dev Biol Anim. 2012 Dec;48(10):610-8. doi: 10.1007/s11626-012-9563-3.
    Species: Rat
    Sample type: Conditioned Media
  7. Chen M., Cheung F., Chan M., et al. Protective rolesofCordycepsonlungfibrosisincellularandratmodels. J Ethnopharmacol. 2012 Sep 28;143(2):448-54. doi: 10.1016/j.jep.2012.06.033
    Species: Rat
    Sample type: Tissue Lysate
  8. Mazo M., Cemborain A., Gavira J., et al. Adipose stromal vascular fraction improves cardiac function in chronic myocardial infarction through differentiation and paracrine activity. Cell Transplant. 2012;21(5):1023-37. doi: 10.3727/096368911X623862
    Species: Rat
    Sample type: Conditioned Media
  9. Buijs M., Geschwing J., Syed L., et al. Spontaneous tumor regression in a syngeneic rat model of liver cancer: implications for survival studies. J Vasc Interv Radiol. 2012 Dec;23(12):1685-91. doi: 10.1016/j.jvir.2012.08.025.
    Species: Rat
    Sample type: Serum
  10. Wirrig C, Hunter I, Mathieson FA, Nixon GF. Sphingosylphosphorylcholine is a proinflammatory mediator in cerebral arteries. Journal of Cerebral Blood Flow & Metabolism. 2011;31(1):212-221. doi:10.1038/jcbfm.2010.79.
    Species: Rat
    Sample type: Conditioned Media
1. Chmiluminescence detection step is quite weird. You have to put membrane in between two plastic covers. However, if you put multiple membranes it may give higher background than single membrane-development.
2. Blocking buffer may not sufficient if your samples need to be highly diluted.
3. All the protocol is considered for one membrane blot; therefore, if you are analyzing multiple membrane, you may have trouble in unequal volume. (If you make 4 mL buffer, you will never get 4 mL.)
4. I wish there is a tool for getting signal intensity. Software does not provide intensity-quantification function.
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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