Rat Cytokine Array C2

RayBio® C-Series Rat Cytokine Antibody Array 2 Kit. Detects 34 Rat Cytokines. Suitable for all liquid sample types.

AAR-CYT-2-2
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Antigen Information

Gene Symbols:
AGER | AGRN | CCL2 | CCL20 | CD86 | CNTF | CSF2 | CX3CL1 | CXCL1 | CXCL2   View more

Assay Format

Number of Targets Detected:
34
Species Detected:
  • Rat
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
4 or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Activin A
Agrin
CD86
(B7-2)
beta-NGF
CINC-1
(CXCL1)
CINC-2
CINC-3
CNTF
Fas Ligand
(TNFSF6)
Fractalkine
(CX3CL1)
GM-CSF
ICAM-1
(CD54)
IFN-gamma
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-1 R6
(IL-1 Rrp2)
IL-2
IL-4
IL-6
IL-10
IL-13
Leptin
LIX
L-Selectin
(CD62L)
MCP-1
(CCL2)
MIP-3 alpha
(CCL20)
MMP-8
PDGF-AA
Prolactin R
RAGE
TCK-1
(CXCL7)
TIMP-1
TNF alpha
VEGF-A

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Rat Cytokine Antibody Array C2 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP??s ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Yang J., Zhou Y., Wei F., Xiao Y. Blood clot formed on rough titanium surface induces early cell recruitment. Clin Oral Implants Res. 2016 Aug;27(8):1031-8. doi: 10.1111/clr.12672.
    Species: Rat
    Sample type: Conditioned Media
  2. Perez-Ramirez I., Enciso-Moreno J., Guevara-Gonzalez R., et al. Modulation of renal dysfunction by Smilax cordifolia and Eryngium carlinae, and their effect on kidney proteome in obese rats. Journal of Functional Foods Volume 20, January 2016, Pages 545-555
    Species: Rat
    Sample type: Tissue lysate
  3. Robles L., Vaziri ND., Li S., et al. Dimethyl fumarate ameliorates acute pancreatitis in rodent. Pancreas. 2015 Apr;44(3):441-7. doi: 10.1097/MPA.0000000000000275.
    Species: Rat
    Sample type: Conditioned Media
  4. Meireles M., Marques C., Norberto S., et al. The impact of chronic blackberry intake on the neuroinflammatory status of rats fed a standard or high-fat diet. J Nutr Biochem. 2015 Nov;26(11):1166-73. doi: 10.1016/j.jnutbio.2015.05.008.
    Species: Rat
    Sample type: Tissue Lysate
  5. de Couto G., Liu W., Tseliou E., et al. Macrophages mediate cardioprotective cellular postconditioning in acute myocardial infarction. J Clin Invest. 2015 Aug 3;125(8):3147-62. doi: 10.1172/JCI81321.
    Species: Rat
    Sample type: Tissue Lysate
  6. Yoshino S., Nakagami G., Ohira T., et al. Hydrocellular foam dressing increases the leptin level in wound fluid. Wound Repair Regen. 2015 Sep;23(5):703-10. doi: 10.1111/wrr.12349.
    Species: Rat
    Sample type: Wound Fluid
  7. Wu, F., Zhang, R. et al. Preliminary Study on Pain Reduction of Monosodium Iodoacetate-Induced Knee Osteoarthritis in Rats by Carbon Dioxide Laser Moxibustion. Evidence-Based Complementary and Alternative Medicine 2014. Epub Ahead of Print. DOI: http://dx.doi.org/10.1155/2014/754304
    Species: Rat
    Sample type: Tissue Lysate
  8. Attia, Mohamed, et al. "Early events of overused supraspinatus tendons involve matrix metalloproteinases and EMMPRIN/CD147 in the absence of inflammation." The American journal of sports medicine 41.4 (2013): 908-917.
    Species: Rat
    Sample type: Tissue Lysate
  9. Vaziri, Nosratola D., et al. "Oral activated charcoal adsorbent (AST-120) ameliorates chronic kidney disease-induced intestinal epithelial barrier disruption." American journal of nephrology 37.6 (2013): 518-525.
    Species: Rat
    Sample type: Plasma
  10. Delucchi F, Berni R, Frati C, et al. Resveratrol Treatment Reduces Cardiac Progenitor Cell Dysfunction and Prevents Morpho-Functional Ventricular Remodeling in Type-1 Diabetic Rats. Pietropaolo M, ed. PLoS ONE 2012;7(6):e39836. doi:10.1371/journal.pone.0039836.
    Species: Rat
    Sample type: Conditioned Media
Very clear signal, easy to quantitate. Always works.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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