Rat Cytokine Array C2

$345.00
AAR-CYT-2-2

RayBio® C-Series Rat Cytokine Antibody Array 2 Kit. Detects 34 Rat Cytokines. Suitable for all liquid sample types.

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information

Activin A
Agrin
CD86
(B7-2)
beta-NGF
CINC-1
(CXCL1)
CINC-2
CINC-3
CNTF
Fas Ligand
(TNFSF6)
Fractalkine
(CX3CL1)
GM-CSF
ICAM-1
(CD54)
IFN-gamma
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-1 R6
(IL-1 Rrp2)
IL-2
IL-4
IL-6
IL-10
IL-13
Leptin
LIX
L-Selectin
(CD62L)
MCP-1
(CCL2)
MIP-3 alpha
(CCL20)
MMP-8
PDGF-AA
Prolactin R
RAGE
TCK-1
(CXCL7)
TIMP-1
TNF alpha
VEGF-A

Species Detected

Rat

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Rat Cytokine Antibody Array C2 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP??s ChemiDoc-It® or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Number of Targets Detected

34

Antigen Information

Gene Symbol:
  • AGER
  • AGRN
  • CCL2
  • CCL20
  • CD86
  • CNTF
  • CSF2
  • CX3CL1
  • CXCL1
  • CXCL2
  • CXCL3
  • CXCL5
  • FASLG
  • ICAM1
  • IFNG
  • IL10
  • IL13
  • IL1A
  • IL1B
  • IL1RL2
  • IL2
  • IL4
  • IL6
  • INHBA
  • LEP
  • MMP8
  • NGF
  • PDGFA
  • PPBP
  • PRLR
  • SELL
  • TIMP1
  • TNF
  • VEGFA

Product Specifications

Size:
4 or 8 Sample Kit

Array/ELISA Format

Species Detected:
  • Rat
Solid Support:
Membrane
Design Principle:
  • Sandwich-based
Quantitative/Semi-Quantitative:
Semi-Quantitative
Method of Detection:
Chemiluminescence
Compatible Sample Types:
  • Serum
  • Plasma
  • Cell Culture Supernatants
  • Cell Lysates
  • Tissue Lysates

  1. Yang J., Zhou Y., Wei F., Xiao Y. Blood clot formed on rough titanium surface induces early cell recruitment. Clin Oral Implants Res. 2016 Aug;27(8):1031-8. doi: 10.1111/clr.12672.
    Species: Rat
    Sample Type: Conditioned Media

  2. Perez-Ramirez I., Enciso-Moreno J., Guevara-Gonzalez R., et al. Modulation of renal dysfunction by Smilax cordifolia and Eryngium carlinae, and their effect on kidney proteome in obese rats. Journal of Functional Foods Volume 20, January 2016, Pages 545-555

  3. Robles L., Vaziri ND., Li S., et al. Dimethyl fumarate ameliorates acute pancreatitis in rodent. Pancreas. 2015 Apr;44(3):441-7. doi: 10.1097/MPA.0000000000000275.
    Species: Rat
    Sample Type: Conditioned Media

  4. Meireles M., Marques C., Norberto S., et al. The impact of chronic blackberry intake on the neuroinflammatory status of rats fed a standard or high-fat diet. J Nutr Biochem. 2015 Nov;26(11):1166-73. doi: 10.1016/j.jnutbio.2015.05.008.
    Species: Rat
    Sample Type: Tissue Lysate

  5. de Couto G., Liu W., Tseliou E., et al. Macrophages mediate cardioprotective cellular postconditioning in acute myocardial infarction. J Clin Invest. 2015 Aug 3;125(8):3147-62. doi: 10.1172/JCI81321.
    Species: Rat
    Sample Type: Tissue Lysate

  6. Yoshino S., Nakagami G., Ohira T., et al. Hydrocellular foam dressing increases the leptin level in wound fluid. Wound Repair Regen. 2015 Sep;23(5):703-10. doi: 10.1111/wrr.12349.
    Species: Rat
    Sample Type: Wound Fluid

  7. Wu, F., Zhang, R. et al. Preliminary Study on Pain Reduction of Monosodium Iodoacetate-Induced Knee Osteoarthritis in Rats by Carbon Dioxide Laser Moxibustion. Evidence-Based Complementary and Alternative Medicine 2014. Epub Ahead of Print. DOI: http://dx.doi.org/10.1155/2014/754304
    Species: Rat
    Sample Type: Tissue Lysate

  8. Delucchi F, Berni R, Frati C, et al. Resveratrol Treatment Reduces Cardiac Progenitor Cell Dysfunction and Prevents Morpho-Functional Ventricular Remodeling in Type-1 Diabetic Rats. Pietropaolo M, ed. PLoS ONE 2012;7(6):e39836. doi:10.1371/journal.pone.0039836.
    Species: Rat
    Sample Type: Conditioned Media

  9. Campbell LR, Pang Y, et al: Intracerebral lipopolysaccharide induces neuroinflammatory change and augmented brain injury in growth-restricted neonatal rats. Pediatr Res. 2012 Jun;71(6):645-52. doi: 10.1038/pr.2012.26.
    Species: Rat
    Sample Type: Tissue Lysate

  10. Grothusen C., Hagemann A., Attmann T., et al. Impact of an Interleukin-1 Receptor Antagonist and Erythropoietin on ExperimentalMyocardial Ischemia/Reperfusion Injury. The ScientificWorld Journal Volume 2012, Article ID 737585, 6 pages doi:10.1100/2012/737585
    Species: Rat
    Sample Type: Serum

  11. Pillon N., Arane K., Bilan P., et al. Muscle cells challenged with saturated fatty acids mount an autonomous inflammatory response that activates macrophages. Cell Commun Signal. 2012 Oct 19;10(1):30. doi: 10.1186/1478-811X-10-30.
    Species: Rat
    Sample Type: Conditioned Media

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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