Cancer Autoantibody Arrays

Introduction

The RayBio^® Cancer Autoantibody Arrays are semi-quantitative multiplex protein arrays developed for the detection of cancer-related auto-antibodies. Recombinant proteins previously associated with cancer are spotted onto a solid glass slide surface, and then probed for the presence of serum-specific antibodies to these targets. These detection kits can then be applied in screening specific autoantibodies based on IgA or IgG levels present in patient serum samples.

For a comparison & overview of all our protein array systems, click here.

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Cancer Auto-antibody Protein Array Features

Get 3x the data, plus high throughput

Each 75mm x 25mm glass slide is spotted with either 8 or 16 identical protein arrays (also called “subarrays”). Within each subarray, the purified protein autoimmunogens along with controls are spotted in triplicate. The slide comes with a 16-well removable gasket and chamber assembly that allows for simultaneous processing of up to 16 samples per slide (8 if using the 8 sample kit). To achieve even higher throughput, 4 slides can be nested into a tray which matches a standard microplate, allowing for automated processing of 64 arrays (If using the 16 sample kit) simultaneously. The latest automated liquid handling work stations can accommodate several trays at once, allowing hundreds of samples to be processed per day.

How It Works

A panel of cancer-related proteins is printed in multiple identical arrays on a standard slide. After a blocking step, samples are incubated with the arrays. Nonspecific antibodies are then washed off, and the arrays are incubated with Biotin-conjugated anti-human IgG or IgA detection antibodies, followed by a streptavidin-conjugated fluor. Signals are then visualized using a fluorescence laser scanner.

For a list of laser scanner specifications, click here.

Testing Services

Full Testing Service: Ship us your samples (5 sample minimum) and our experienced service department will run the array and analyze the data for you. Email our tech support team at [email protected] to learn more.

Scanning Service: If you do not have access to a scanner, RayBiotech provides slide scanning and data analysis services

RayBiotech Protein Array slide scanning and data extraction service is now FREE for a limited time only! Simply ship us your slide/s and we will scan the slide/s and send you the raw data extracted from the image. Click to learn more

Need help analyzing all that data? Copy and paste your data into the RayBiotech Analysis Tool specific for your array. The analysis will automatically perform background subtraction and normalization.

Research Applications

High-throughput profiling of auto-antibodies in human serum

Lung Cancer IgG Auto-antibody Array G1

Contains 30 human proteins for simultaneous detection of human IgG autoantibodies associated with lung cancer:

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Lung Cancer IgA Auto-antibody Array G1

Contains 30 human proteins for simultaneous detection of human IgA autoantibodies associated with lung cancer:

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