Cancer Autoantibody Arrays

Introduction

The RayBio® Cancer Auto-antibody Protein Arrays are semi-quantitative multiplex protein immunoassays developed for the detection of Cancer related auto-antibodies. Recombinant proteins are spotted onto a solid glass slide surface, and then probed for the presence of serum specific antibodies to these targets. These detection kits can then be applied in screening specific autoantibodies based on IgA or IgG levels present in patient serum samples

For a comparison & overview of all our protein array systems, click here.

 

Browse Cancer Auto-antibody Arrays    Compare Protein Array Target Lists    

 
 

Cancer Auto-antibody Protein Array Features

Multichannel PipetteGet 3x the data, plus high throughput

Each 75mm x 25mm glass slide is spotted with either 8 or 16 identical protein arrays (also called  “subarrays”). Within each subarray, the purified protein autoimmunogens along with controls are spotted in triplicate. The slide comes with a 16-well removable gasket and chamber assembly that allows for simultaneous processing of up to 16 samples per slide (8 if using the 8 sample kit). To achieve even higher throughput, 4 slides can be nested into a tray which matches a standard microplate, allowing for automated processing of 64 arrays (If using the 16 sample kit) simultaneously. The latest automated liquid handling work stations can accommodate several trays at once, allowing hundreds of samples to be processed per day. 

Features

 

Contents of kit

  • Less sample, more data: only 2 ul of sample is needed for detection of 30 cancer-related autoantibodies
  • Less than 8-hour processing time
  • No interference between immobilized proteins (unlike bead-based multiplex assays)
  • No dedicated equipment required
 
    • Glass Chip with Protein Arrays
    • Biotin-conjugated Anti-human IgG or IgA
    • Blocking Buffer
    • Streptavidin-Fluorescent dye
    • Wash buffers
    • Manual

 *Accessories include: 16-well incubation chamber with gasket, protective cover, snap-on sides, adhesive film

  

How It Works

A panel of cancer-related proteins is printed in multiple identical arrays on a standard slide. After a blocking step, samples are incubated with the arrays. Nonspecific antibodies are then washed off, and the arrays are incubated with Biotin-conjugated anti-human IgG or IgA detection antibodies, followed by a streptavidin-conjugated fluor. Signals are then visualized using a fluorescence laser scanner.

For a list of laser scanner specifications, click here.

Cancer Auto-antibody Protein Array - How it works

 

Testing Services

Full Testing Service: Ship us your samples (5 sample minimum) and our experienced service department will run the array and analyze the data for you. Email our tech support team at techsupport@raybiotech.com to learn more.

  

Scanning Service: If you do not have access to a scanner, RayBiotech provides slide scanning and data analysis services

RayBiotech Protein Array slide scanning and data extraction service is now FREE for a limited time only! Simply ship us your slide/s and we will scan the slide/s and send you the raw data extracted from the image. Click to learn more

Need help analyzing all that data? Copy and paste your data into the RayBiotech Analysis Tool specific for your array. The analysis will automatically perform background subtraction and normalization.

Research Applications

  • High-throughput profiling of auto-antibodies in human serum
 

Lung Cancer IgG Auto-antibody Array G1

Contains 30 human proteins for simultaneous detection of human IgG autoantibodies associated with lung cancer:

Scroll over each spotted protein for more information

14-3-3 theta
  • Gene Symbol: YWHAQ
  • Gene ID: 10971
  • Accession #: P27348
Annexin A1
Annexin A2
  • Gene Symbol: ANXA2 ANX2 ANX2L4 CAL1H LPC2D
  • Gene ID: 302
  • Accession #: P07355
CAGE
  • Gene Symbol: DDX53 CAGE
  • Gene ID: 168400
  • Accession #: Q86TM3
Cathepsin D
CDK2
  • Gene Symbol: CDK2 CDKN2
  • Gene ID: 1017
  • Accession #: P24941
c-Myc
(MYC)
Cyclin A
  • Gene Symbol: CCNA2 CCN1 CCNA
  • Gene ID: 890
  • Accession #: P20248
Cyclin B1
  • Gene Symbol: CCNB1 CCNB
  • Gene ID: 891
  • Accession #: P14635
Cyclin D1
  • Gene Symbol: CCND1
  • Accession #: Q6FI00
DKK-1
GBU4-5
  • Gene Symbol: TDRD12
  • Gene ID: 25044
  • Accession #: Q587J7
HSP70-9B/HSPA9/GRP-75
  • Gene Symbol: HSPA9 GRP75 HSPA9B mt-HSP70
  • Gene ID: 3313
  • Accession #: P38646
Hu-D
  • Gene Symbol: ELAVL4 HUD PNEM
  • Gene ID: 1996
  • Accession #: P26378
IMDH2
  • Gene Symbol: IMPDH2 IMPD2
  • Gene ID: 3615
  • Accession #: P12268
IMP1
  • Gene Symbol: IGF2BP1 CRDBP VICKZ1 ZBP1
  • Gene ID: 10642
  • Accession #: Q9NZI8
IMP2/IGF2BP2/p62
  • Gene Symbol: IGF2BP2 IMP2 VICKZ2
  • Gene ID: 10644
  • Accession #: Q9Y6M1
IMP3
  • Gene Symbol: IGF2BP3 IMP3 KOC1 VICKZ3
  • Gene ID: 10643
  • Accession #: O00425
LAMR1
  • Gene Symbol: RPSA LAMBR LAMR1
  • Gene ID: 3921
  • Accession #: P08865
MAGEA4
  • Gene Symbol: MAGEA4 MAGE4
  • Gene ID: 4103
  • Accession #: P43358
MALAT1
  • Gene Symbol: MALAT1
  • Gene ID: 29665
  • Accession #: Q9UHZ2
NY-ESO-1
  • Gene Symbol: CTAG1A CTAG CTAG1 ESO1 LAGE2 LAGE2A; CTAG1B LAGE2B
  • Gene ID: 1485; 246100
  • Accession #: P78358
p53
Paxillin
  • Gene Symbol: PXN
  • Gene ID: 9718
  • Accession #: P49023-2
PGAM1
  • Gene Symbol: PGAM1 PGAMA CDABP0006
  • Gene ID: 5223
  • Accession #: P18669
PGP9.5
  • Gene Symbol: UCHL1
  • Gene ID: 7345
  • Accession #: P09936
Recoverin
  • Gene Symbol: RCVRN RCV1
  • Gene ID: 5957
  • Accession #: P35243
SOX2
Survivin
(BIRC5)
  • Gene Symbol: BIRC5 API4 IAP4
  • Gene ID: 332
  • Accession #: O15392
Ubiquilin-1
  • Gene Symbol: UBQLN1 DA41 PLIC1
  • Gene ID: 29979
  • Accession #: Q9UMX0

View Product Page

 

Lung Cancer IgA Auto-antibody Array G1

Contains 30 human proteins for simultaneous detection of human IgA autoantibodies associated with lung cancer:

Scroll over each spotted protein for more information

14-3-3 theta
  • Gene Symbol: YWHAQ
  • Gene ID: 10971
  • Accession #: P27348
Annexin A1
Annexin A2
  • Gene Symbol: ANXA2 ANX2 ANX2L4 CAL1H LPC2D
  • Gene ID: 302
  • Accession #: P07355
CAGE
  • Gene Symbol: DDX53 CAGE
  • Gene ID: 168400
  • Accession #: Q86TM3
Cathepsin D
CDK2
  • Gene Symbol: CDK2 CDKN2
  • Gene ID: 1017
  • Accession #: P24941
c-Myc
(MYC)
Cyclin A
  • Gene Symbol: CCNA2 CCN1 CCNA
  • Gene ID: 890
  • Accession #: P20248
Cyclin B1
  • Gene Symbol: CCNB1 CCNB
  • Gene ID: 891
  • Accession #: P14635
Cyclin D1
  • Gene Symbol: CCND1
  • Accession #: Q6FI00
DKK-1
GBU4-5
  • Gene Symbol: TDRD12
  • Gene ID: 25044
  • Accession #: Q587J7
HSP70-9B/HSPA9/GRP-75
  • Gene Symbol: HSPA9 GRP75 HSPA9B mt-HSP70
  • Gene ID: 3313
  • Accession #: P38646
Hu-D
  • Gene Symbol: ELAVL4 HUD PNEM
  • Gene ID: 1996
  • Accession #: P26378
IMDH2
  • Gene Symbol: IMPDH2 IMPD2
  • Gene ID: 3615
  • Accession #: P12268
IMP1
  • Gene Symbol: IGF2BP1 CRDBP VICKZ1 ZBP1
  • Gene ID: 10642
  • Accession #: Q9NZI8
IMP2/IGF2BP2/p62
  • Gene Symbol: IGF2BP2 IMP2 VICKZ2
  • Gene ID: 10644
  • Accession #: Q9Y6M1
IMP3
  • Gene Symbol: IGF2BP3 IMP3 KOC1 VICKZ3
  • Gene ID: 10643
  • Accession #: O00425
LAMR1
  • Gene Symbol: RPSA LAMBR LAMR1
  • Gene ID: 3921
  • Accession #: P08865
MAGEA4
  • Gene Symbol: MAGEA4 MAGE4
  • Gene ID: 4103
  • Accession #: P43358
MALAT1
  • Gene Symbol: MALAT1
  • Gene ID: 29665
  • Accession #: Q9UHZ2
NY-ESO-1
  • Gene Symbol: CTAG1A CTAG CTAG1 ESO1 LAGE2 LAGE2A; CTAG1B LAGE2B
  • Gene ID: 1485; 246100
  • Accession #: P78358
p53
Paxillin
  • Gene Symbol: PXN
  • Gene ID: 9718
  • Accession #: P49023-2
PGAM1
  • Gene Symbol: PGAM1 PGAMA CDABP0006
  • Gene ID: 5223
  • Accession #: P18669
PGP9.5
  • Gene Symbol: UCHL1
  • Gene ID: 7345
  • Accession #: P09936
Recoverin
  • Gene Symbol: RCVRN RCV1
  • Gene ID: 5957
  • Accession #: P35243
SOX2
Survivin
(BIRC5)
  • Gene Symbol: BIRC5 API4 IAP4
  • Gene ID: 332
  • Accession #: O15392
Ubiquilin-1
  • Gene Symbol: UBQLN1 DA41 PLIC1
  • Gene ID: 29979
  • Accession #: Q9UMX0

View Product Page