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| CODE | Number of Plates | Price | ||
|---|---|---|---|---|
|
ELC-EGFR-5
|
$1,920.00 | ||
|
|
ELC-EGFR-1
|
$480.00 | ||
|
|
ELC-EGFR-2
|
$864.00 |
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Antigen Information
Accession Number:
- Q6QT04
Antigen Name & Synonyms:
Epidermal growth factor receptor (Fragment)
Gene Names:
- EGFR
Assay Format
Detection Range:
6.4 pg/ml - 2500 pg/ml
Quantitative/Semi-Quantitative:
Quantitative
Recommended Dilution (serum & plasma):
200 fold
Sensitivity:
6.4 pg/ml
Specificity:
This ELISA kit shows no cross-reactivity with the following cytokines tested: canine Cystatin C, Decorin, IL-1 alpha, IL-1ra, IL-13, IL-21, RANTES (CCL5), Resistin, Trappin-2 (Elafin).
Number of Targets Detected:
1
Species Detected:
- Canine (dog)
Compatible Sample Types:
- Cell Culture Supernatants
- Plasma
- Serum
Design Principle:
- Sandwich-based
Method of Detection:
Colorimetric
Solid Support:
96-well Microplate
Product Specifications
Size:
1, 2, or 5 x 96-Well Strip Microplate Kit
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
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Usage
This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.
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