ELISA (recommended work dilution= 1 : 32,000)
The immunogen was a synthetic peptide (PA83). This antibody was produced from a chicken immunized with this peptide conjugated with KLH. The IgY fraction was purified from collected egg followed by affinity chromatography.
A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial.
Bacillus anthracis, a spore-forming bacterium, is the causative agent of anthrax. Virulent strains are encapsulated and secrete two toxins composed of three proteins: the protective antigen 83 (PA83; 83 kDa), the lethal factor (LF; 85 kDa), and the edema factor (EF; 89 kDa). Intravenous injection of the lethal toxin (Letx; PA83 plus LF) into animals causes sudden death. PA83 binds via domain 4 to a ubiquitous proteinaceous cell receptor which has been identified as the anthrax toxin receptor). PA83 is then cleaved at a site in domain 1 by furin-like proteases into PA63 and PA20 (a 20-kDa amino-terminal fragment). This processing leads to the heptamerization of PA63 (24) and the subsequent binding of EF or LF. PA83 is therefore a key protein, promoting both the binding of the toxins to the cell surface and the translocation of their enzymatic moieties into the cytoplasm of the host cell.
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
This antibody specifically binds to Bacillus anthracis PA83.
- Leppla, S. H. 1995. Anthrax toxins, p. 543-563. In J. Moss, B. Iglewski, M. Vaughan, and A. T. Tu (ed.), Bacterial toxins and virulence factors in disease, vol. 8. Handbook of natural toxins.
- Marcel Dekker, New York, N.Y. Fabien Brossier, et al. Functional Analysis of Bacillus anthracis Protective Antigen by Using Neutralizing Monoclonal Antibodies. Infect Immun. 72(11): 63136317.
12 months from the date of shipment when stored properly.