The immunogen was a synthetic peptide (PA83). This antibody was produced from a chicken immunized with this peptide conjugated with KLH. The IgY fraction was purified from collected egg followed by affinity chromatography.
Product is supplied as a powder obtained from lyophilization of purified antibody in PBS without preservatives. Reconstitute the antibody with sterile 1 x PBS to a final concentration of 1 mg/ml.
Bacillus anthracis, a spore-forming bacterium, is the causative agent of anthrax. Virulent strains are encapsulated and secrete two toxins composed of three proteins: the protective antigen 83 (PA83; 83 kDa), the lethal factor (LF; 85 kDa), and the edema factor (EF; 89 kDa). Intravenous injection of the lethal toxin (Letx; PA83 plus LF) into animals causes sudden death. PA83 binds via domain 4 to a ubiquitous proteinaceous cell receptor which has been identified as the anthrax toxin receptor). PA83 is then cleaved at a site in domain 1 by furin-like proteases into PA63 and PA20 (a 20-kDa amino-terminal fragment). This processing leads to the heptamerization of PA63 (24) and the subsequent binding of EF or LF. PA83 is therefore a key protein, promoting both the binding of the toxins to the cell surface and the translocation of their enzymatic moieties into the cytoplasm of the host cell.
This antibody specifically binds to Bacillus anthracis PA83.
Store at 4°C if intended for use within one month, otherwise, store at -20°C to -80°C. The lyophilized antibody is stable for at least 18 months after the date of receipt when stored at -20°C to -80°C. After reconstitution, it can also be aliquoted and stored frozen at -20°C to -80°C in a manual defrost freezer for at least 6 months without detectable loss of activity. Upon reconstitution, the antibody can also be stored for 30 days at 4°C. Please avoid freeze-thaw cycles, as this will lower the activity of the antibody.
- Leppla, S. H. 1995. Anthrax toxins, p. 543-563. In J. Moss, B. Iglewski, M. Vaughan, and A. T. Tu (ed.), Bacterial toxins and virulence factors in disease, vol. 8. Handbook of natural toxins.
- Marcel Dekker, New York, N.Y. Fabien Brossier, et al. Functional Analysis of Bacillus anthracis Protective Antigen by Using Neutralizing Monoclonal Antibodies. Infect Immun. 72(11): 63136317.
12 months from the date of shipment when stored properly.