ELISA (recommended work dilution= Up to 1:160,000)
Immunogen was bacterial extract from Geobacter sulfurreducens. This antibody was produced from a chicken immunized with the immunogen. The IgY fraction was purified from collected egg followed by in-house affinity chromatography.
Supplied as lyophilized and purified antibody originally containing PBS, without Preservative Stabilizers, such as Sodium Azide. The final concentration is indicated on the shipping vial. The antibody is stable for at least years from the data of receipt when stored at -20°C to -70°C. Reconstituted antibody (recommended with H20 1 mg/ml) can also be aliquoted and stored frozen at -20°C to -70°C in a manual defrost freezer for months without detectable loss activity. Upon reconstitution, the antibody can be stored at 4°C for 1 month. Please avoid freeze-thaw cycles.
The antibody can specifically bind to its immunogen, and did not show any cross reactivity with unrelated antigens as did in ELISA. The specificity for binding to recombinant protein, cellular protein and native antigen was not defined. Cross reactivity with other related bacterial antigens was not tested yet.
Store at -20°C, if not intended for use within a month, keep it at 4°C and minimize freezing and thawing when use.
- Janet B. Rollefson, Camille S. Stephen, Ming Tien, and Daniel R. Bond,(2011). Identification of an Extracellular Polysaccharide Network Essential for Cytochrome Anchoring and Biofilm Formation in Geobacter sulfurreducens. Journal of Bacteriology, 193(5):1023-1033.
- Yu Qiu, Byung-Kwan Cho,Young Seoub Park,Derek Lovley,Bernhard Ã. Palsson and Karsten Zengler,Structural and operational complexity of the Geobacter sulfurreducens genome. Genome Res. 2010. 20: 1304-1311.
12 months from the date of shipment when stored properly.