ELISA (recommended work dilution= 1: 5,000-10,000), Western Blotting
Immunogen was recombinant protein derived from human procalcitonin. This antibody was produced from a chicken immunized with the immunogen. The IgG fraction was purified from chicken serum followed by Protein A/G affinity chromatography.
A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial.
Procalcitonin (PCT) is a peptide precursor of the hormone calcitonin. The mature calcitonin is composed of 116 amino acids and plays an important role in calcium homeostasis. Calcitonin is mainly produced by parafollicular C cells of the thyroid and the endocrine cells of the intestine and lung. Calcitonincauses a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones. Procalcitonin levels may be useful to distinguish bacterial infections from nonbacterial infections.
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
The antibody specifically binds to the immunogen and did not show any cross reactivity with unrelated antigens in ELISA. The specificity for binding to recombinant protein, cellular protein and native antigen is not defined. Cross reactivity with mouse and rat procalcitonin has not yet been tested.
Rabbit Anti-Human Procalcitonin (cat# 130-00025 )Recombinant Human Procalcitonin (cat# 228-11317-3 ) Mouse Anti-Procalcitonin / Katacalcin (cat# MD-14-0114)
- Jereb M et al.. (2009) Usefulness of procalcitonin for diagnosis of infective endocarditis. Intern Emerg Med.4(3):221-6.
- Prat C et al.. (2008) Evaluation of procalcitonin, neopterin, C-reactive protein, IL-6 and IL-8 as a diagnostic marker of infection in patients with febrile neutropenia. Leuk Lymphoma. 49(9):1752-61.