Chymotrypsin, Alpha, TLCK Treated, Sequencing Grade

Three times crystallized and treated with 1-chloro-3-tosylamido-7-amino-2-heptanone (TLCK) to inhibit trypsin activity [Shaw, et al., Biochemistry, 4, 2219, (1965)]. Dialyzed to remove autolysis products and low molecular weight contaminants. Supplied as lyophilized powder.

$243.00  
252-10111-1
+

Product Specifications

Biological activity:
≥45 units per milligram protein
Protease Type:
Chymotrypsin
Recommended Applications:
Sequence analysis Peptide synthesis Peptide mapping Peptide fingerprinting

Protein Information

Accession Number:
  • P00766

Preparation

Source:
Bovine Pancreas
Molecular Weight:
25.6 kDa (Wilcox 1970)

Other Information

Protease sequencing grade
Yes
Extinction Coefficient
51,840 cm-1 M-1 (Theoretical) E1%,280 Equals 20.19 (Chymotrypsinogen, Theoretical) E1%,280 Equals 20.57 (Chymotrypsin, Theoretical)
Isoelectric
8.52 (Chymotrypsinogen, Theoretical) 8.33 (Chymotrypsin, Theoretical)
CATH
Class: Mainly Beta Architecture: Beta Barrel Topology: Trypsin-like Serine Protease
CAS Registry Number
C.A.S.: 9004-07-3
Storage Conditions
Store at 2-8°C

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.