The COVID-19 (SARS-CoV-2) Real Time RT-PCR 1-Step Nucleic Acid Detection Kit is based on the PCR method which uses a fluorescent probe and a specific primer to detect two specific regions within the novel coronavirus (SARS-CoV-2) nucleocapsid protein N gene. This molecular panel aids in the detection of viral RNA from SARS-CoV-2, the causative agent of COVID-19. The kit also contains a proprietary buffer which enables the user to use crude saliva or throat swabs directly in their PCR reactions. By simply mixing the 1-Step Nucleic Acid Stabilization Buffer with the sample, the viral RNA is immediately stabilized, and interfering components of the saliva matrix are inactivated. This eliminates the need for laborious, costly RNA extraction and purification steps, drastically improving upon the traditional workflow of PCR, while removing potential sample loss and contamination.
The kit includes 3 primer-probe sets corresponding to those used in the CDC 2019-Novel Coronavirus (2019-nCoV) Real-Time RT-PCR Diagnostic Panel (cat no. 2019-nCoVEUA-01). Primer-probe sets N1 and N2 detect SARS-CoV-2 specifically, with no expected false positives from other coronaviruses or human microflora. The kit also includes a primer-probe set specific to the to the human housekeeping gene, ribonuclease P (RNP), are included to serve as an internal reference to monitor sample collection, RNA extraction, and amplification.
|Primers and Probe N1 Solution||Primers & probe for N1||140 µL / tube||1 tube|
|Primers and Probe N2 Solution||Primers & probe for N2||140 µL / tube||1 tube|
|Primers and Probe RP Solution||Primers & probe for Human RNAase P||140 µL / tube||1 tube|
|PCR Reaction Solution||Buffer, dNTP's||12000 µL / tube||1 tube|
|PCR Enzyme Mix||Reverse transcriptase (RT) enzyme, DNA polymerase||96 µL / tube||1 tube|
|Positive Control||Plasmid DNA containing target gene (Nucleocapsid protein) + plasmid DNA containing internal control gene fragment (RNP)||480 µL / tube||1 tube|
|Negative Control||Nuclease-free water||480 µL / tube||1 tube|
|One-Step Nucleic Acid Stabilization Buffer||Buffer||1.5mL / tube||2 tubes|