COVID-19 (SARS-CoV-2) Real Time RT-PCR 1-Step Nucleic Acid Detection Kit

30 samples. Suitable for the qualitative detection of COVID-19 (SARS-CoV-2) nucleocapsid protein N gene in human saliva and throat swabs. Enables the user to use crude saliva or swabs directly in PCR reactions, no extraction needed. Conforms to CDC guidelines. LDT ready for high complexity CLIA labs.


Antigen Information

Target Name:
N1; N2; RNP

Assay Format

Number of Targets Detected:
Compatible Sample Types:
  • Saliva
  • Throat swab
Method of Detection:
Real-Time RT-PCR
  • Qualitative

Product Specifications

30 Samples


The COVID-19 (SARS-CoV-2) Real Time RT-PCR 1-Step Nucleic Acid Detection Kit is based on the PCR method which uses a fluorescent probe and a specific primer to detect two specific regions within the novel coronavirus (SARS-CoV-2) nucleocapsid protein N gene. This molecular panel aids in the detection of viral RNA from SARS-CoV-2, the causative agent of COVID-19. The kit also contains a proprietary buffer which enables the user to use crude saliva or throat swabs directly in their PCR reactions. By simply mixing the 1-Step Nucleic Acid Stabilization Buffer with the sample, the viral RNA is immediately stabilized, and interfering components of the saliva matrix are inactivated. This eliminates the need for laborious, costly RNA extraction and purification steps, drastically improving upon the traditional workflow of PCR, while removing potential sample loss and contamination.

The kit includes 3 primer-probe sets corresponding to those used in the CDC 2019-Novel Coronavirus (2019-nCoV) Real-Time RT-PCR Diagnostic Panel (cat no. 2019-nCoVEUA-01). Primer-probe sets N1 and N2 detect SARS-CoV-2 specifically, with no expected false positives from other coronaviruses or human microflora. The kit also includes a primer-probe set specific to the to the human housekeeping gene, ribonuclease P (RNP), are included to serve as an internal reference to monitor sample collection, RNA extraction, and amplification.

Kit Components

Component Ingredients Specification Quantity
Primers and Probe N1 Solution Primers & probe for N1 140 µL / tube 1 tube
Primers and Probe N2 Solution Primers & probe for N2 140 µL / tube 1 tube
Primers and Probe RP Solution Primers & probe for Human RNAase P 140 µL / tube 1 tube
PCR Reaction Solution Buffer, dNTP's 12000 µL / tube 1 tube
PCR Enzyme Mix Reverse transcriptase (RT) enzyme, DNA polymerase 96 µL / tube 1 tube
Positive Control Plasmid DNA containing target gene (Nucleocapsid protein) + plasmid DNA containing internal control gene fragment (RNP) 480 µL / tube 1 tube
Negative Control Nuclease-free water 480 µL / tube 1 tube
One-Step Nucleic Acid Stabilization Buffer Buffer 1.5mL / tube 2 tubes