The Coronavirus (SARS-CoV-2) Real Time RT-PCR Nucleic Acid Detection Kit is based on the PCR method which uses a fluorescent probe and a specific primer to detect three specific regions within the novel coronavirus (SARS-CoV-2) nucleocapsid protein N gene. This molecular panel aids in the detection ofviral RNA from SARS-CoV-2, the causative agent of COVID-19. The kit includes 3 primer-probe sets corresponding to those used in the CDC 2019-Novel Coronavirus (2019-nCoV) Real-Time RT-PCR Diagnostic Panel (cat no. 2019-nCoVEUA-01).
Primer-probe sets N1 and N2 detect SARS-CoV-2 specifically, with no expected false positives fromother coronaviruses or human microflora.The kit also includes a primer-probe set specific to the to the human housekeeping gene, ribonuclease P (RNP), are included to serve as an internal reference to monitor sample collection, RNA extraction, and amplification.
|Primers and Probe N1 Solution||Primers & probe for N1||140 µL / tube||1 tube|
|Primers and Probe N2 Solution||Primers & probe for N2||140 µL / tube||1 tube|
|Primers and Probe RP Solution||Primers & probe for Human RNAase P||140 µL / tube||1 tube|
|PCR Reaction Solution||Buffer, dNTP's||12000 µL / tube||1 tube|
|PCR Enzyme Mix||Reverse transcriptase (RT) enzyme, DNA polymerase||96 µL / tube||1 tube|
|Positive Control||Plasmid DNA containing target gene (Nucleocapsid protein) + plasmid DNA containing internal control gene fragment (RNP)||480 µL / tube||1 tube|
|Negative Control||Nuclease-free water||480 µL / tube||1 tube|