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Coronavirus (SARS-CoV-2) Real Time RT-PCR Nucleic Acid Detection Kit

20 tests. Suitable for the qualitative detection of COVID-19 (SARS-CoV-2) nucleocapsid protein N gene in human throat swabs and alveolar lavage. Includes a one-step RT-qPCR mix. Conforms to CDC guidelines. LDT ready for high complexity CLIA labs.

$697.00
PCR-COV
+

Antigen Information

Target Name:
N1; N2; RNP

Assay Format

Number of Targets Detected:
3
Compatible Sample Types:
  • Broncheoalveolar lavage
  • Throat swab
Method of Detection:
Real-Time RT-PCR
Quantitative/Semi-Quantitative:
Qualitative

Product Specifications

Size:
20 Tests

Introduction

The Coronavirus (SARS-CoV-2) Real Time RT-PCR Nucleic Acid Detection Kit is based on the PCR method which uses a fluorescent probe and a specific primer to detect three specific regions within the novel coronavirus (SARS-CoV-2) nucleocapsid protein N gene. This molecular panel aids in the detection ofviral RNA from SARS-CoV-2, the causative agent of COVID-19. The kit includes 3 primer-probe sets corresponding to those used in the CDC 2019-Novel Coronavirus (2019-nCoV) Real-Time RT-PCR Diagnostic Panel (cat no. 2019-nCoVEUA-01).

Primer-probe sets N1 and N2 detect SARS-CoV-2 specifically, with no expected false positives fromother coronaviruses or human microflora.The kit also includes a primer-probe set specific to the to the human housekeeping gene, ribonuclease P (RNP), are included to serve as an internal reference to monitor sample collection, RNA extraction, and amplification.

Kit Components

Component Ingredients Specification Quantity
Primers and Probe N1 Solution Primers & probe for N1 140 µL / tube 1 tube
Primers and Probe N2 Solution Primers & probe for N2 140 µL / tube 1 tube
Primers and Probe RP Solution Primers & probe for Human RNAase P 140 µL / tube 1 tube
PCR Reaction Solution Buffer, dNTP's 12000 µL / tube 1 tube
PCR Enzyme Mix Reverse transcriptase (RT) enzyme, DNA polymerase 96 µL / tube 1 tube
Positive Control Plasmid DNA containing target gene (Nucleocapsid protein) + plasmid DNA containing internal control gene fragment (RNP) 480 µL / tube 1 tube
Negative Control Nuclease-free water 480 µL / tube 1 tube

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