COVID-19 S-Protein (S1RBD) ELISA Kit

SARS-CoV-2 (COVID-19) S-Protein (S1RBD) ELISA Kit. Detects the S-Protein (S1RBD). Standard protein code: 230-30162.

Lead time: Typically ships within 1-2 business days. No Friday shipments.


Assay Format

Detection Range:
6.14 pg/ml - 1500 pg/ml
Recommended Dilution (serum & plasma):
4 fold
4.5 pg/ml
This ELISA antibody pair detects COVID-19 S Protein (S1RBD).
Number of Targets Detected:
Species Detected:
  • Virus
Compatible Sample Types:
  • Cell Culture Supernatants
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

1, 2, or 5 x 96-Well Strip Microplate Kit

Protein Information

Gene Names:
  • S
Protein Name & Synonyms:
SARS-CoV-2 Spike Protein, S1 Subunit, S protein, spike protein

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
The kit arrived very quickly and was very easy to use following the manual. I used plasma samples from various groups of patients and the results came out very nicely!

Boston Children's Hospital
Got it very quickly after ordering and arrived in perfect condition. Would recommend!

University of Arkansas
After success with RayBiotech's N-Protein specific ELISA kit, the RBD specific kits proved to be just as easy to use. Human SARS-CoV-2 convalescent sera samples reacted well at several dilutions. These kits are also competitively priced compared to others. Standard curves are consistent and all reagents are clearly labeled and organized. Would use this kit again!

Mayo Clinic
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This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.