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COVID-19 Spike-ACE2 Binding Assay Kit

7 reviews
Citations (5)

The RayBio® COVID-19 Spike-ACE2 binding assay kit is a patented, rapid, simple, and sensitive method to characterize the binding affinity of the SARS-CoV-2 Spike (S) protein and the Angiotensin I Converting Enzyme 2 (ACE2) receptor complex in the presence of potential inhibitors.

Positive and negative controls for the Spike-ACE2 binding assays are available as an accessory item. Click here.

$487.00  
CoV-SACE2-1
+ −
Note:
The binding assay comes in two different options based on which protein is immobilized on the plate and which protein is added in solution (i.e. "query"). It is the researcher's responsibility to select the format for their study.

Assay Format

Number of Targets Detected:
1
Species Detected:
  • Human
  • SARS-CoV-2
Compatible Sample Types:
  • Antibodies
  • Peptides
  • Plasma
  • Proteins
  • Serum
  • Small Molecules
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Protein Information

Accession Number:
  • QHD43416
Gene Symbols:
  • ACE2
  • RBD
  • S
  • S-RBD
  • SRBD
Protein Name & Synonyms:
Angiotensin-converting enzyme 2, ACE-related carboxypeptidase, Angiotensin-converting enzyme homolog, ACEH, Metalloprotease MPROT15, Processed angiotensin-converting enzyme 2, ACE2, Spike protein, S Protein, S1 Subunit, Host Cell Receptor Binding Domain (RBD)

Validation Data

Inhibition of Spike-ACE2 by anti-viral compounds
Inhibition of Spike-ACE2 by anti-viral compounds
Four small molecules tested for neutralizing activity with COVID-19 Spike-ACE2 Binding Assay (COV-SACE2).
Inhibition of Spike-ACE2 by anti-Spike antibodies
Inhibition of Spike-ACE2 by anti-Spike antibodies
Four antibodies tested for neutralizing activity with COVID-19 Spike-ACE2 Binding Assay (COV-SACE2). nAb 1 = 130-10807; nAb 2 = 130-10844; nAb 3 = 130-10845; negative control = 130-10813.

Application Notes

  • Evaluate inhibitor activity
  • Screen inhibitors of the SARS-CoV-2 Spike-ACE2 complex
  • Characterize antibody function
  • Vaccine development
  • Selection of patient serum for antibody therapy

Storage/Stability

Store kit at 4°C for up to 1 month from date of shipment.

  1. Pirovano, G., Ordonez, A. A., Jain, S. K., Reiner, T., Carroll, L. S., & Pillarsetty, N. V. K. (2021). Rapid detection of SARS-CoV-2 using a radiolabeled antibody. Nuclear Medicine and Biology, 98-99, 69–75. doi:10.1016/j.nucmedbio.2021.05.1
    Species:
    Sample type:
  2. Tsukinoki, Keiichi et al. "Detection of cross-reactive IgA against SARS-CoV-2 spike 1 subunit in saliva." medRxiv 2021.03.29.21253174; doi: https://doi.org/10.1101/2021.03.29.21253174
    Species:
    Sample type: Purified antibody
  3. Boroduske, Anete et al. "Wild Sambucus nigra L. from north-east edge of the species range: A valuable germplasm with inhibitory capacity against SARS-CoV2 S-protein RBD and hACE2 binding in vitro." Industrial crops and products vol. 165 (2021): 113438. doi:10.1016/j.indcrop.2021.113438
    Species:
    Sample type: Fruit extract
  4. Olaleye, Omonike A et al. "Discovery of Clioquinol and analogues as novel inhibitors of Severe Acute Respiratory Syndrome Coronavirus 2 infection, ACE2 and ACE2 - Spike protein interaction in vitro." Heliyon vol. 7,3 (2021): e06426. doi:10.1016/j.heliyon.2021.e06426
    Species:
    Sample type: Small molecule
  5. Olaleye, Omonike A et al. "Ambroxol Hydrochloride Inhibits the Interaction between Severe Acute Respiratory Syndrome Coronavirus 2 Spike Protein’s Receptor Binding Domain and Recombinant Human ACE2." bioRxiv 2020.09.13.295691; doi: https://doi.org/10.1101/2020.09.13.295691
    Species:
    Sample type: Small molecule
The kit is great, we have use 3 kits already. Something extra to say not product related itself, is BE CAREFUL with the temperature labeled in the shipping box. All items I've got from RayBiotech has came with a -20C storage recommendation when the actual kit temp is 4C and few items are -20. My coworker didnt check and all my kit got into -20 overnight until I came next day and verify it.
John S.
Stanford University 05/17/2021, 06:06 PM
Kit doesn't come with kind of protocol but, works very well.
Nitin
The University of Cincinnati 04/29/2021, 04:20 PM
Some of the wells did work well. Next time we are planning to do an overnight incubation to give it a better chance. I'm ordering a 2nd kit.
John S.
Stanford University 04/21/2021, 05:24 PM
we tested the transduction efficiency of the viruses on wild type 293 cells... plasma samples were used as negative.
D Yun
NANOBST 03/17/2021, 10:32 AM
I checked inhibition of SARS-CoV2 spike protein and ACE2 interaction by using using our developed vaccine and it worked really well.
Ananya
Washington University in St. Louis 03/15/2021, 12:57 AM
Kit helped us to check whether the our test compound is inhibiting the interaction or not
Raj Nandani
South dakota state university 03/10/2021, 02:42 PM
Positive control along with kit showed a good signal. However, our RBD antigen showed no binding with secondary Abs. So finally we used competitive ELISA, it looks good though. Now it is the 2nd kit we are using, good product.
Haiyue Xu
University of Texas at Austin 01/31/2021, 08:41 PM
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# Manual - Spike RBD on plate + ACE2 query (CoV-SACE2)

# Manual - ACE2 on plate + Spike RBD query (CoV-ACE2S2)

# SDS - Spike RBD on plate + ACE2 query (CoV-SACE2)

# SDS - ACE2 on plate + Spike RBD query (CoV-ACE2S2)

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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