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COVID19 High Throughput Real Time RT-PCR Nucleic Acid Detection Kit

80 tests. Suitable for the qualitative in vitro detection of novel coronavirus (SARS-CoV-2) nucleic acid in patient sera and respiratory specimens (including pharyngeal swabs and alveolar lavage fluid samples).

$560.00  
PCR-COVHT
+

Antigen Information

Target Name:
N, ORF1ab, RNP

Assay Format

Number of Targets Detected:
3
Compatible Sample Types:
  • Broncheoalveolar lavage
  • Throat swab
Method of Detection:
Real-Time RT-PCR
Quantitative/Semi-Quantitative:
Qualitative

Product Specifications

Size:
80 Tests

Introduction

The Coronavirus (SARS-CoV-2) HighThroughput Real Time RT-PCR Nucleic Acid Detection Kit is based on the PCR method which uses a fluorescent probeand a specific primer to detect two specific regions within the novel coronavirus (SARS-CoV-2) RNA genome. One target correspondstothe nucleocapsid protein N gene region, and the second target corresponds to the ORF1ab region. This molecular panel aids in the detection of viral RNA from SARS-CoV-2, the causative agent of COVID-19.

Primer-probe sets for the N and ORF1ab detect SARS-CoV-2 specifically, with no expected false positives from other coronaviruses or human microflora. The kit also includes a primer-probe set specific to the to the human housekeeping gene, ribonuclease P (RNP), are included to serve as an internal reference to monitor sample collection, RNA extraction, and amplification.

This kit combines the 3 primer-probe sets into a single reaction, reducing the number of wells and reagents needed by as much as 75% compared to other RT-PCR assays. The N and ORF1ab primer-probe sets are both detected using a 494/518nm (FITC or FAM) channel, and the RP gene is detected using 533/544nm channel.

Kit Components

Component Ingredients Specification Quantity
PCR Reaction Solution Buffer, dNTP's, Primers, and Probes 1300 µL / tube 1 tube
PCR Enzyme Mix Reverse transcriptase (RT) enzyme, DNA polymerase 96 µL / tube 1 tube
Positive Control Stabilized RNA for the N gene and plasmid DNA for the RP gene 100 µL / tube 4 tubes
Negative Control Nuclease-free water 100 µL / tube 1 tube