The Coronavirus (SARS-CoV-2) HighThroughput Real Time RT-PCR Nucleic Acid Detection Kit is based on the PCR method which uses a fluorescent probeand a specific primer to detect two specific regions within the novel coronavirus (SARS-CoV-2) RNA genome. One target correspondstothe nucleocapsid protein N gene region, and the second target corresponds to the ORF1ab region. This molecular panel aids in the detection of viral RNA from SARS-CoV-2, the causative agent of COVID-19.
Primer-probe sets for the N and ORF1ab detect SARS-CoV-2 specifically, with no expected false positives from other coronaviruses or human microflora. The kit also includes a primer-probe set specific to the to the human housekeeping gene, ribonuclease P (RNP), are included to serve as an internal reference to monitor sample collection, RNA extraction, and amplification.
This kit combines the 3 primer-probe sets into a single reaction, reducing the number of wells and reagents needed by as much as 75% compared to other RT-PCR assays. The N and ORF1ab primer-probe sets are both detected using a 494/518nm (FITC or FAM) channel, and the RP gene is detected using 533/544nm channel.
|PCR Reaction Solution||Buffer, dNTP's, Primers, and Probes||1300 µL / tube||1 tube|
|PCR Enzyme Mix||Reverse transcriptase (RT) enzyme, DNA polymerase||96 µL / tube||1 tube|
|Positive Control||Stabilized RNA for the N gene and plasmid DNA for the RP gene||100 µL / tube||4 tubes|
|Negative Control||Nuclease-free water||100 µL / tube||1 tube|