CRISPR-Associated Protein-9 Nuclease S. Pyogenes Recombinant

$1,552.00
228-20845-2



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Source

Accession Number:
  • Q99ZW2
Protein Name & Synonyms:
CRISPR-associated endonuclease Cas9/Csn1, SpyCas9, cas9, csn1.

Preparation

Expression System:
E.Coli
Purity:
Greater than 90% as determined by SDS-PAGE.

Protein Specifications

Format:
Sterile Filtered colorless solution.
Formulation:
The CAS9 solution contains 10mM Tris-HCl, 300mM NaCl, 0.1mM EDTA, 50% glycerol and 1mM DTT pH 7.5.

Description

Recombinant Cas9-NLS produced in E.coli, comprises the entire Cas9 protein sequence (1368 amino acids) joined to a proprietary nuclear localization sequence (NLS) and a 6xHis tag at the C-terminus, having a total of 1414 amino acids. The protein has an apparent molecular weight of 163kDa.

Stability & Storage

Storage/Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.